In-silico study of single-strand DNA aptamers against cortisol as a potential biomarker receptor in therapeutics and diagnostics

Sabri, M.; Hamid, Azzmer Azzar Abdul; Hitam, Sharifah Mariam Sayed; Rahim, Mohd. Zulkhairi Abdul

doi:10.1016/j.matpr.2020.12.1080

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…The aptasensor showed higher sensitivity to cortisol at pH 5, so the construction of the analytical curve, applying the aptasensor in artificial saliva, and studying storage time were carried out at pH 5. This behavior showed that the composite material used as supporting material probably affects the aptamer's recognition process, which it can be compared with the aptamer free in solution [13].…”

Section: Resultsmentioning

confidence: 99%

“…These bond distances are classified as strong, covalently formed bonds. In addition to hydrogen bonds, the distance of hydrophobic interactions influences the stability of the complexes formed [13]. After immobilization of Ap on the platform, an electrostatic repulsion may occur between Ap and the redox probe [Fe(CN) 6 ] 3−/4− , both negatively charged [32].…”

Section: Resultsmentioning

confidence: 99%

“…The negative charge of the phosphate groups is greatly influenced by the medium's ionic strength, as observed in previous studies. Still, the stability of the conformational structure of the aptamer ligand can also change with the pH of the solution [13]. Figure 4 illustrates the results of the measurements performed in 5 mmol L −1 Na 4 Fe(CN) 6 solution in Tris/HCl 50 mmol L −1 , MgCl 2 10 mmol L −1 , and 10 mmol L −1 NaCl support electrolyte, varying the pH.…”

Section: Resultsmentioning

confidence: 99%

“…For this reason, there is a great interest in developing biosensors using aptamers (Ap). Ap are single‐stranded DNA or RNA oligonucleotides (ssDNA or RNA) ranging from 15 to 100 nucleotides in length with the ability to bind specifically to the target molecules, such as cortisol [13]. These bioreceptors show very high affinity for their targets, with dissociation constants typically in 10 −6 to 10 −12 [14, 15].…”

Section: Introductionmentioning

confidence: 99%

“…These bioreceptors show very high affinity for their targets, with dissociation constants typically in 10 −6 to 10 −12 [14, 15]. After interacting with the target molecule, the aptamer changes its conformation, altering the charge transfer resistance values of the system [13, 16].…”

Section: Introductionmentioning

confidence: 99%

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A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

2023

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Cortisol is a steroid hormone naturally produced by the adrenal glands. It participates in and controls several processes in the body and is considered an important physiological biomarker. Due to its very low concentrations in body fluids, its detection requires high sensitivity and specificity. Here, we present a simple electrochemical biosensor based on reduced graphene oxide (rGO) modified with gold nanoparticles (AuNPs) for the immobilization of the cortisol‐specific aptamer (Ap) (MCH‐Ap‐AurGO/GCE). Important analytical parameters for identifying the target analyte were optimized, such as conditions and amount of immobilized Ap, the influence of the concentration and nature of the supporting electrolyte, pH of the medium, and incubation time. The optimized conditions for the aptasensor were: concentration of Ap 1.0 × 10−6 mol L−1, support electrolyte Tris/HCl 50 mmol L−1, MgCl2 10 mmol L−1, and NaCl 10 mmol L−1, at pH 5.0 and incubation time of 15 min. A linear response range was obtained from 1 × 10−18 up to 1 × 10−11 mol L−1 of cortisol with a detection limit (LOD) of 1.0 × 10−18 mol L−1. A curve adjusted for operational purposes in the saliva sample was fitted for the concentration range between 0.5 × 10−14 and 1 × 10−11 mol L−1, with a linear regression equation ΔRtc/Rtc1 = 2.70 + 0.17 × log([Cortisol]). The aptasensor demonstrated a great potential for detecting cortisol in a simple, fast, and highly sensitive way, opening its path for application in real samples, which present levels below the concentration in which cortisol is commonly found in body fluids.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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