In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Li, Yabei; Ye, Zhaoyang; Zhang, Junjie; Zhao, Yifeng; Zhu, Tong; Song, Jingjing; Xu, Feng; Li, Fei

doi:10.1021/acs.analchem.2c01919

Cited by 11 publications

(9 citation statements)

References 53 publications

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“…Scanning electrochemical microscopy (SECM) uses an electrochemical scanning probe microscope with a μm/nm-sized electrode as its probe to record various charge transfer processes around cells. − Due to the advantages of high temporal and spatial resolutions and sample contactless scanning properties, SECM has been used to in situ monitor a variety of parameters of cancer cells at a single-cell level and in a noninvasive manner. − For example, the membrane permeability, respiratory activity, and ROS level of cancer cells were quantitatively measured by SECM using [Ru(NH 3 ) 6 ]Cl 3 , oxygen, and H 2 O 2 as the redox mediators in the SECM system. ,,− However, in previous studies, , when applying the constant potentials of oxygen reduction and H 2 O 2 oxidation/reduction at the SECM probe, the continuous depletion of oxygen and H 2 O 2 around cells occurred, which would perturb the normal cellular physiological microenvironment and thus influence cell function. Recently, He’s group developed a programmable pulse potential mode of SECM, , which can avoid continuous oxygen and other redox mediator consumption by the SECM probe, thus realizing the characterization of multiple targets during a single process.…”

Section: Introductionmentioning

confidence: 99%

In Situ and Quantitatively Monitoring the Dynamic Process of Ferroptosis in Single Cancer Cells by Scanning Electrochemical Microscopy

Zhao

Kuermanbayi

et al. 2023

Anal. Chem.

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Ferroptosis, as a promising therapeutic strategy for cancers, has aroused great interest. Quantifying the quick dynamic changes in key parameters during the early course of ferroptosis can provide insights for understanding the underlying mechanisms of ferroptosis and help the development of therapies targeting ferroptosis. However, in situ and quantitatively monitoring the quick responses of living cancer cells to ferroptosis at the single-cell level remains technically challenging. In this work, we selected HuH7 cells (hepatocellular carcinoma (HCC) cells) as a cell model and Erastin as a typical ferroptosis inducer. We utilized scanning electrochemical microscopy (SECM) to quantitatively and in situ monitor the early course of ferroptosis in HuH7 cells by characterizing the three key parameters of cell ferroptosis (i.e., cell membrane permeability, respiratory activity, and the redox state). The SECM results show that the membrane permeability of ferroptotic HuH7 cells continuously increased from 0 to 8.1 × 10 −5 m s −1 , the cellular oxygen consumption was continuously reduced by half, and H 2 O 2 released from the cells exhibited periodic bursts during the early course of ferroptosis, indicating the gradually destroyed cell membrane structure and intensified oxidative stress. Our work realizes, for the first time, the in situ and quantitative monitoring of the cell membrane permeability, respiratory activity, and H 2 O 2 level of the early ferroptosis process of a single living cancer cell with SECM, which can contribute to the understanding of the physiological process and underlying mechanisms of ferroptosis.

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Section: Introductionmentioning

confidence: 99%

In Situ and Quantitatively Monitoring the Dynamic Process of Ferroptosis in Single Cancer Cells by Scanning Electrochemical Microscopy

Zhao

Kuermanbayi

et al. 2023

Anal. Chem.

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“…Scanning electrochemical microscopy (SECM) is an electrochemical scanning probe microscopy technique utilizing a micro-/nanoelectrode as its probe to scan the sample surface by recording the Faradic current of a redox mediator around the sample in an electrolyte solution . Owing to its in situ, noninvasive, and label-free advantages, SECM has been employed for detecting the oxygen consumption, membrane permeability, and contraction frequency of various living cells and tissues. , For instance, Komatsu et al used SECM to in situ monitor the dynamic changes in contraction frequency and oxygen concentration of cardiomyocytes under different temperatures and drug treatments . In our recent work, we employed SECM to quantitatively measure the metabolic and electrophysiological properties of living cardiac tissues in situ .…”

Section: Introductionmentioning

confidence: 99%

Effect of Exogenous Electric Stimulation on the Cardiac Tissue Function In Situ Monitored by Scanning Electrochemical Microscopy

Zhao

et al. 2023

Anal. Chem.

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Cardiac tissue is sensitive to and can be easily damaged by exogenous electric stimulation. However, due to the thermal−electric coeffect and the limitation of in situ and quantitative information on the cardiac tissue function under electric stimulation, the detailed effect and the underlying mechanism of exogenous electric stimulation on the cardiac tissue remain elusive. To address this, in this work, we first constructed an in vitro cardiac tissue model and established a thermal−electric coupled theoretical model for simulating the electric field and temperature distributions around the cardiac tissue, from which we selected the electric field strengths (1.19, 2.37, and 3.39 kV cm −1 ) and electrical energies (0.001, 0.005, and 0.011 J) for electric stimulations without inducing a thermal effect. Then, we applied electric field stimulations on the cardiac tissue using these parameters and scanning electrochemical microscopy (SECM) to in situ and quantitatively monitor the dynamic changes in the key parameters of the cardiac tissue function, including respiratory activity, membrane permeability, and contraction frequency, after electric field stimulations. The SECM results showed that the oxygen consumption, cell membrane permeability coefficient, and contraction frequency of the cardiac tissue were strongly dependent on electrical energy, especially when the electrical energy was higher than 0.001 J. Our work, for the first time, achieves the in situ and quantitative monitoring of the cardiac tissue function under electric stimulation using SECM, which would provide important references for designing an electric stimulation regime for cardiac tissue engineering and clinical application of electrotherapy.

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“…Electrochemical mapping is typically carried out in DC amperometric mode, and redox species are added to the electrolyte, which produces current at the interface of a mobile micro-electrode. In close proximity to the surface of interest, fluctuation in the current provides valuable information regarding surface topography and its chemical composition ( Lin et al, 2018 ; Li et al, 2022 ). Xia et al (2019) used a similar technique where electrochemical noise from current or potential fluctuations can produce specific signatures related to the rupture of organic coatings.…”

Section: Introductionmentioning

confidence: 99%

“…This high-resolution AC-SECM impedance mapping can be performed over a range of frequencies, and AC current flow during the measurement is induced by the superimposition of a sinusoidal waveform over a constant DC potential ( Diakowski and Ding, 2007 ). An added advantage of this technique is that it does not require the addition of a redox mediator into the electrolyte solution ( Li et al, 2022 ). The major advantage of this technique lies in the ability to identify microscopic domains with differential electrochemical activity which can be exploited to investigate biological surfaces ( Polcari et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy

Vyas

Kotla²,

Rochev³

et al. 2023

Front. Bioeng. Biotechnol.

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Alternating current scanning electrochemical microscopy (AC-SECM) is a powerful tool for characterizing the electrochemical reactivity of surfaces. Here, perturbation in the sample is induced by the alternating current and altered local potential is measured by the SECM probe. This technique has been used to investigate many exotic a range of biological interfaces including live cells and tissues, as well as the corrosive degradation of various metallic surfaces, etc. In principle, AC-SECM imaging is derived from electrochemical impedance spectroscopy (EIS) which has been used for a century to describe interfacial and diffusive behaviour of molecules in solution or on a surface. Increasingly bioimpedance centric medical devices have become an important tool to detect evolution of tissue biochemistry. Predictive implications of measuring electrochemical changes within a tissue is one of the core concepts in developing minimally invasive and smart medical devices. In this study, cross sections of mice colon tissue were used for AC-SECM imaging. A 10 micron sized platinum probe was used for two-dimensional (2D) tan δ mapping of histological sections at a frequency of 10 kHz, Thereafter, multifrequency scans were performed at 100 Hz, 10 kHz, 300 kHz, and 900 kHz. Loss tangent (tan δ) mapping of mice colon revealed microscale regions within a tissue possessing a discrete tan δ signature. This tan δ map may be an immediate measure of physiological conditions in biological tissues. Multifrequency scans highlight subtle changes in protein or lipid composition as a function of frequency which was recorded as loss tangent maps. Impedance profile at different frequencies could also be used to identify optimal contrast for imaging and extracting the electrochemical signature specific for a tissue and its electrolyte.

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In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Cited by 11 publications

References 53 publications

In Situ and Quantitatively Monitoring the Dynamic Process of Ferroptosis in Single Cancer Cells by Scanning Electrochemical Microscopy

In Situ and Quantitatively Monitoring the Dynamic Process of Ferroptosis in Single Cancer Cells by Scanning Electrochemical Microscopy

Effect of Exogenous Electric Stimulation on the Cardiac Tissue Function In Situ Monitored by Scanning Electrochemical Microscopy

Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy

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