In Situ Antibody Detection and Charge Discrimination Using Aqueous Stable Pentacene Transistor Biosensors

Abstract

Pentacene-based organic thin-film transistors were used to create highly sensitive, real-time electronic sensors for selective antibody detection. Bovine serum albumin was covalently attached to a modified pentacene surface to selectively detect the label free monoclonal antiBSA. These sensors displayed a high affinity constant (K(A)) of (1.1 ± 3) × 10(7) M(-1) at pH 7, which is 1 order of magnitude higher than those obtained with a highly sensitive surface plasmon resonance spectroscopy detection system. Furthermore, a high degree of discrimination in the hybrid antiBSA charges was achieved at different pH values. This demonstration of fast, label-free, real-time detection of nanoscale biomolecules in aqueous buffer solutions using the organic transistor sensing platform will have a significant impact on high-performance microarrays in addition to discriminating the presence of ionizable groups.

Effect of passivation on the sensitivity and stability of pentacene transistor sensors in aqueous media

“…Details of the device fabrication, functionalization, and experimental conditions used here are described in detail elsewhere and can be found in SI section (Khan et al, 2010(Khan et al, , 2011. Briefly, we fabricated a bottom-contact OTFT with 30 nm of pentacene as the active layer on a polymer dielectric with a width (W) of 500 m and length (L) of 50 m (Fig.…”

“…1a. Additional details pertaining to the fabrication procedure are given in supporting information (SI) section (Khan et al, 2011). The polymerization of perfluor-1-3-dimethylcyclohexam and maleic anhydride was carried out in a custom-built PE-CVD reactor following a well-established procedure in our laboratory (Khan et al, 2010(Khan et al, , 2011.…”

“…Additional details pertaining to the fabrication procedure are given in supporting information (SI) section (Khan et al, 2011). The polymerization of perfluor-1-3-dimethylcyclohexam and maleic anhydride was carried out in a custom-built PE-CVD reactor following a well-established procedure in our laboratory (Khan et al, 2010(Khan et al, , 2011. Three different thicknesses were used on top of the transistor devices: H1 consisting of 50 nm PE-CVD of PFDMCH polymer, H2 consisting of 300 nm of spin-coated CYTOP polymer, and H3 consisting of 600 nm of spin-coated CYTOP polymer.…”

A Review on Security Issues in Wireless Sensor Network

“…Both approaches continue to offer solutions for fast, multiplexed, simple and cheap detection of oligonucleotides, PCR amplicons, genomic DNA (fragments), etc. [1]. Most likely, the competition will never see a single winner that meets all needs because the different practical formats and boundary conditions for applications, as well as, market requirements may ask for specific and unique solutions that could be better achieved in one case by optics and in another situation by electronics.…”

Effects of piezoresistivity of pentacene channel in organic thin film transistors under mechanical bending

“…[5][6][7][8][9][10][11] For example, pentacene thin film transistors (TFTs) have been investigated in flexible backplane display devices that require great endurance, [1,2] as well as in mechanical strain, pressure, thermal and bio sensing applications. [12][13][14][15][16][17][18][19][20][21] Although pentacene TFTs with the capability of enduring mechanical deformation have been demonstrated, some related phenomena such as changes in output current, leakage current and field-effect channel mobility during bending have also been observed, [22,23] although without thorough investigations. Therefore, an evaluation of strain effect on pentacene thin film transistors (TFTs) is essential.…”