In situ bioprinting of the skin for burns

Binder, Kyle W.; Zhao, Weixin; Aboushwareb, Tamer; Dice, Dennis D.; Atala, Anthony; Yoo, James J.

doi:10.1016/j.jamcollsurg.2010.06.198

Cited by 112 publications

(78 citation statements)

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“…Binder et al demonstrated their in situ inkjet-based 3D skin printer with which they directly repaired the skin defects on rats [6,7] . The results indicated that multiple skin cells could be directly delivered onto a wound with an acceptable cell survival rate through the in situ printing procedure.…”

Section: Current Status Of In Vivo Bioprintingmentioning

confidence: 99%

The trend towards in vivo bioprinting

Wang¹,

He²,

Liu³

et al. 2024

IJB

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Bioprinting is one of several newly emerged tissue engineering strategies that hold great promise in alleviating of organ shortage crisis. To date, a range of living biological constructs have already been fabricated in vitro using this technology. However, an in vitro approach may have several intrinsic limitations regarding its clinical applicability in some cases. A possible solution is in vivo bioprinting, in which the de novo tissues/organs are to be directly fabricated and positioned at the damaged site in the living body. This strategy would be particularly effective in the treatment of tissues/organs that can be safely arrested and immobilized during bioprinting. Proof-of-concept studies on in vivo bioprinting have been reported recently, on the basis of which this paper reviews the current state-of-the-art bioprinting technologies with a particular focus on their advantages and challenges for the in vivo application.

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Section: Current Status Of In Vivo Bioprintingmentioning

confidence: 99%

The trend towards in vivo bioprinting

Wang¹,

He²,

Liu³

et al. 2024

IJB

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“…[82] Researchers at the Wake Forest Institute for Regenerative Medicine have developed modified ink-jet technology to build av ariety of tissue and organ prototypes by arranging multiple cell types and other tissue components in predetermined locations with high precision. [83] Thee ffect of the printing conditions,i ncluding substrate stiffness, [84] surfactant concentration, and agitation, on printed cells has also been investigated. [85] It has been shown that the substrate onto which the cell droplet is deposited should be soft enough to absorb the kinetic energy of droplets so that the impact force on the cells is reduced substantially.…”

Section: Inkjet-based Bioprintingmentioning

confidence: 99%

3D Bioprinting of Tissue/Organ Models

2016

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In vitro tissue/organ models are useful platforms that can facilitate systematic, repetitive, and quantitative investigations of drugs/chemicals. The primary objective when developing tissue/organ models is to reproduce physiologically relevant functions that typically require complex culture systems. Bioprinting offers exciting prospects for constructing 3D tissue/organ models, as it enables the reproducible, automated production of complex living tissues. Bioprinted tissues/organs may prove useful for screening novel compounds or predicting toxicity, as the spatial and chemical complexity inherent to native tissues/organs can be recreated. In this Review, we highlight the importance of developing 3D in vitro tissue/organ models by 3D bioprinting techniques, characterization of these models for evaluating their resemblance to native tissue, and their application in the prioritization of lead candidates, toxicity testing, and as disease/tumor models.

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“…In situ skin printing is challenging as it requires the development of dedicated bioprinting devices, the integration with imaging systems to obtain data from the wound site, and the design of bioinks capable of instructing printed cells to perform Cell liver spheroids Extrusion Liver [185] their native functions. Binder et al [18,184] developed a device for the in situ skin printing composed of a cartridge delivery system containing a series of inkjet nozzles and a laser scanning system, both mounted on a portable XYZ plotting system. The data obtained from the laser was used to reconstruct a 3D model of the wound, which was subsequently employed to determine the skin area that was missing from the wound.…”

Section: Printed Skin Constructs For Wound Healingmentioning

confidence: 99%

“…Afterwards, the printheads filled the wound site with a bioink containing skin cells in a layer-by-layer manner. The potential of the system to induce the skin regeneration was firstly evaluated through the printing of human keratinocytes and fibroblasts suspended in a fibrinogen-collagen precursor solution into a full thickness skin lesions (3.0 9 2.5 cm 2 ) created on nu/ nu mice [18]. After printing each layer, a thrombin solution was sprayed on top of the deposited layer to induce hydrogel formation.…”

Section: Printed Skin Constructs For Wound Healingmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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In situ bioprinting of the skin for burns

Cited by 112 publications

References 0 publications

The trend towards in vivo bioprinting

The trend towards in vivo bioprinting

3D Bioprinting of Tissue/Organ Models

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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