In situ cannulation, microgrid follow-up and low-density plating provide first passage endothelial cell masscultures for in vitro lining

Vascular Grafts and the Endothelium

“…For example, in two-stage procedures, the use of low endothelial cell plating densities (1.5 × 10 3 cells/cm 2 ) results in shorter cycles than plating cells at higher densities (1 × 10 4 ) and allows sufficient cell numbers to plate a long vascular prosthesis after about 26 days from harvest from a human saphenous vein [118]. Interestingly, cultured cells have been shown to be superior to freshly acquired endothelial cells in seeding grafts and coating grafts with fibronectin, collagen, or plasma also improved seeding efficiency [110,[119][120][121].…”

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

“…Its impact was felt in early vascular tissue engineering. The “purity” of cultured endothelial cells ( 79 ) for instance, was seen as key to the clinical success of vascular graft endothelialisation as “contaminating” smooth muscle cells were assumed to be the nucleus of occlusive intimal hyperplasia. It took long-term clinical explants to disprove this paradigm.…”

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

“…When ECs are seeded, they result in a low surface density (HERRING et al, 1984;ZILLA et al, 1987). Higher-density seeding, known as sodding, results in improved clinical results but requires cell culture, limiting this source to two-stage seeding regimens ZILLA et al, 1990).…”