In-situ monitoring of breast cancer cell (MCF-7) growth and quantification of the cytotoxicity of anticancer drugs fluorouracil and cisplatin

Xiao, Xilin; Guo, Minzhe; Li, Qiangxiang; Cai, Qingyun; Yao, Shouzhuo; Grimes, Craig A.

doi:10.1016/j.bios.2008.03.041

Cited by 37 publications

(19 citation statements)

References 20 publications

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“…Although molecular biologic end-point assays on 3D cultures are in general more laborious and/or can be easily falsified when the readout (e.g., absorbance or fluorescence) is performed directly on the 3D culture, label-free bioelectronic methods have recent advantages. Especially, impedance spectroscopy is a suitable technique for characterization of single tumor cells (14) as well as cellular degradation in 2D cultures (15,16) and, more strikingly, for quantitative monitoring of chemotherapeutic efficacy in 3D cultures (17,18). Using our unique microcavity array (MCA) technology, we could detect the sensitivity or even an aggressive response to certain chemotherapeutics within 3D in vitro tumor models (19).…”

Section: Introductionmentioning

confidence: 99%

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

Jahnke¹,

Poenick²,

Maschke

et al. 2014

Cancer Research

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Stage III/IV melanoma remains incurable in most cases due to chemotherapeutic resistance. Thus, predicting and monitoring chemotherapeutic responses in this setting offer great interest. To overcome limitations of existing assays in evaluating the chemosensitivity of dissociated tumor cells, we developed a label-free monitoring system to directly analyze the chemosensitivity of undissociated tumor tissue. Using a preparation of tumor micro-fragments (TMF) established from melanoma biopsies, we characterized the tissue organization and biomarker expression by immunocytochemistry. Robust generation of TMF was established successfully and demonstrated on a broad range of primary melanoma tumors and tumor metastases. Organization and biomarker expression within the TMF were highly comparable with tumor tissue, in contrast to dissociated, cultivated tumor cells. Using isolated TMF, sensitivity to six clinically relevant chemotherapeutic drugs (dacarbazine, doxorubicin, paclitaxel, cisplatin, gemcitabine, and treosulfan) was determined by impedance spectroscopy in combination with a unique microcavity array technology we developed. In parallel, comparative analyses were performed on monolayer tumor cell cultures. Lastly, we determined the efficacy of chemotherapeutic agents on TMF by impedance spectroscopy to obtain individual chemosensitivity patterns. Our results demonstrated nonpredictable differences in the reaction of tumor cells to chemotherapy in TMF by comparison with dissociated, cultivated tumor cells. Our direct impedimetric analysis of melanoma biopsies offers a direct ex vivo system to more reliably predict patient-specific chemosensitivity patterns and to monitor antitumor efficacy. Cancer Res; 74(22); 6408-18. Ó2014 AACR.

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Section: Introductionmentioning

confidence: 99%

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

Jahnke¹,

Poenick²,

Maschke

et al. 2014

Cancer Research

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“…This polymer has been used by other authors in the literature [33][34][35][36][37][38] as a protective layer of the magnetoelastic ribbons due to its excellent binding properties to the metallic glass surfaces and its long-term stability in aqueous environments where most of the polymers fail. We discovered that BAYHYDROL-110 shows a very positive response to several VOCs, with various sensitivities.…”

Section: Methodsmentioning

confidence: 98%

“…The advantages of using magnetoelastic sensors are (a) the remote sensing by means of pick-up coils, (b) the sensor's low price which can be used on a disposable basis, and (c) the low power and cost of the equipment. Further information concerning the principles of operation, as well as the design and applications of magnetoelastic sensors [34][35][36][37], can be found in the review of Grimes at al. [21] and in the references mentioned therein.…”

Section: Introductionmentioning

confidence: 98%

A polymer-Metglas sensor used to detect volatile organic compounds

Baimpos

Boutikos

Nikolakis

et al. 2010

Sensors and Actuators A: Physical

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“…They are mostly referred to as an inductor/capacitor (LC) sensor, which integrates a series-connected interdigital capacitor (IDC) with a spiral inductor printed on a glass substrate or silicon substrate [8][9][10][11][12]. A wireless magnetic sensing device with LC resonant-circuit was developed for the in-situ monitoring of the growth of human breast cancer cells [13], detection of E. coli O157:H7 [14], or monitoring the bacterial contamination of milk [15]. All these methods are mostly based on the impedance measurement.…”

Section: Introductionmentioning

confidence: 99%

Inductance-based sensing technique for wireless, remote-query measurement in liquid media

Nie

Huang

et al. 2010

Sci. China Chem.

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A novel inductance-based sensing technique is presented for remote query measurement in different liquid media including organic solvents and inorganic solutions. The inorganic solutions tested included salt solutions of different concentrations, and the organic solvents detected included 1,4-dioxane and tetrahydrofuran. To extend the application of the sensor, bacterial culture media were also detected, and the growth of Escherichia coli (E. coli) was controlled. The influential factors which may affect the inductance responses were studied in detail. It was found that quantitative relationships exist between the sensor's inductance response and the physico-chemical parameters of the liquid media. The sensor's inductance response (L) decreases with the increase of salt concentration (C) and its ionic valence (e) according to a semi-logarithmic equation LgL = aeC + b, where a and b are constants, which is in accordance with the theoretically deduced equation. The inductance variation rate (K) increases directly with the temperature (T): K = a′ T + b′. As for organic solutions, the sensor's inductance was found to increase with the increasing permittivity of the organic solution. The wireless sensor we designed is simple and easy to manipulate. It has the potential for remote determination of not only chemical substances but also microbiological species such as bacteria. Using the newly developed inductance-based sensor, the pathogenic E. coli was monitored with a limit of detection of 10 cells/mL and a linear semi-logarithmic range of 1.0 × 10 1 to 2.5 × 10 9 cells/mL. inductance sensor, remote-query, liquid medium, wireless, bacterial detection, E. coli.

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In-situ monitoring of breast cancer cell (MCF-7) growth and quantification of the cytotoxicity of anticancer drugs fluorouracil and cisplatin

Cited by 37 publications

References 20 publications

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

A polymer-Metglas sensor used to detect volatile organic compounds

Inductance-based sensing technique for wireless, remote-query measurement in liquid media

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