In vitro activities of natural products against oral Candida isolates from denture wearers

Abstract

BackgroundCandida-associated denture stomatitis is a frequent infectious disease. Treatment of this oral condition is difficult because failures and recurrences are common. The aim of this study was to test the in vitro antifungal activity of pure constituents of essentials oils.MethodsEight terpenic derivatives (carvacrol, farnesol, geraniol, linalool, menthol, menthone, terpinen-4-ol, and α-terpineol), a phenylpropanoid (eugenol), a phenethyl alcohol (tyrosol) and fluconazole were evaluated against 38 Candida isolated from denture-wearers and 10 collection Candida strains by the CLSI M27-A3 broth microdilution method.ResultsAlmost all the tested compounds showed antifungal activity with MIC ranges of 0.03-0.25% for eugenol and linalool, 0.03-0.12% for geraniol, 0.06-0.5% for menthol, α-terpineol and terpinen-4-ol, 0.03-0.5% for carvacrol, and 0.06-4% for menthone. These compounds, with the exception of farnesol, menthone and tyrosol, showed important in vitro activities against the fluconazole-resistant and susceptible-dose dependent Candida isolates.ConclusionsCarvacrol, eugenol, geraniol, linalool and terpinen-4-ol were very active in vitro against oral Candida isolates. Their fungistatic and fungicidal activities might convert them into promising alternatives for the topic treatment of oral candidiasis and denture stomatitis.

Lamiaceae Peppermint Oil with Surfactant Showing Equal Antifungal Activity against <i>Candida albicans</i> to Rosemary Chemotype CINEOL

“…Menthone and menthol are known as main components of peppermint [14] [15]. These two components are reported to act against C. albicans based on a study on their MICs and MFCs using broth microdilution method [16]. Linalool and linalyl acetate are the main components of lavender and clary sage [17]- [19].…”

Tratamento da estomatite protética: revisão de literatura

“…New therapies are being proposed for the treatment of denture stomatitis, through the use of natural products, 7,8 nanomaterials 9 and photodynamic therapy. 10 However, the treatment of choice for denture stomatitis consists, mostly of hygiene instructions of the prosthesis, of the application of a topical antifungal and verification of the need to make a new prosthesis.…”

Avaliação dos possíveis mecanismos de ação dos probióticos contra Candida spp. isoladas de usuários de próteses totais

“…Esta lesão eritematosa, observada geralmente no palato duro sob uma prótese total, tem causa multifatorial [10,11,40]. Seu aparecimento pode estar associado à utilização de próteses antigas mal adaptadas [41,42], ou a presença de um biofilme inespecífico [17].…”

“…Os diferentes graus de inflamação palatal, que podem ocorrer como resultado do uso de próteses, são normalmente associados a presença de um grande número de micro-organismos do gênero Candida no espaço entre a mucosa que recobre o palato e a superfície da prótese [11,36,40], sendo assim as próteses funcionam como um reservatório onde estes micro-organismos se proliferam causando a infecção [43,44].…”

“…A remoção diária do biofilme presente nas próteses totais é de suma importância para minimizar a estomatite sob prótese, além de contribuir para o estado de saúde geral do paciente [57]. Para reduzir as frequentes recidivas [40,44], a troca periódica das próteses também é recomendada.…”