In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria

Boyanova, Lyudmila; Kolarov, Rossen; Gergova, Galina; Mitov, Ivan

doi:10.1016/j.anaerobe.2006.06.001

Cited by 47 publications

(35 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results are presented as MIC values (Table 1), except for e. coli since no antibacterial activity could be detected and all MIC values were above 50 mg/ml. Nina et al (2015) described limited activity of propolis samples from Argentina against e. coli, while others (Boyanova et al 2006;Kosalec et al 2007) found no inhibitory activity of Bulgarian and Brazilian propolis, respectively, against e. coli. Kosalec et al (2007) found MIC values of below 10.4 mg/ml against e. coli while testing Croatian propolis samples.…”

Section: Discussionmentioning

confidence: 99%

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

Gajger¹,

Pavlović²,

Bojić³

et al. 2017

Czech J. Food Sci.

View full text Add to dashboard Cite

Tlak Gajger I., Pavlović I., Bojić M., Kosalec I., Srečec S., Vlainić T., Vlainić J. Propolis is a popular subject of research worldwide due to its therapeutic potential. The antimicrobial activity of propolis appears to be promising but depends on many variables related to its origin such as the content of phenolics and flavonoids. To address this issue with Croatian propolis, which has two major origins (Mediterranean and continental), we exposed bacteria (escherichia coli and staphylococcus aureus) and yeasts (candida albicans and Aspergillus niger) to different propolis concentrations (two-fold microdilution method with TCC/formazan endpoint). Total phenolic and flavonoid content and chromatographic profile along with antioxidant activity were assessed. The majority of the 24 propolis samples tested exhibited potent antimicrobial activity against s. aureus bacteria and the yeast c. albicans. Most propolis samples also exhibited robust antioxidative capacity which correlated polyphenol and flavonoid content. To the best of our knowledge this is the first study in which the antimicrobial activity of Croatian propolis is correlated with its constituents.

show abstract

Section: Discussionmentioning

confidence: 99%

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

Gajger¹,

Pavlović²,

Bojić³

et al. 2017

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…As Kačániová et al (2012) reported that feeding of propolis to chickens increased enterococcus and lactobacillus in excreta, propolis residue seems to have the potency to increase the growth of some kinds of bacteria. Boyanova et al (2006) reported that Bulgarian propolis was highly active against the anaerobic bacteria including Prevotellaceae. As shown in Tables 1 and 2, the antibacterial effect against the family Prevotellaceae was also found in the propolis residue collected from Iwate prefecture in Japan.…”

Section: Discussionmentioning

confidence: 99%

Influence of Propolis Residue on the Bacterial Flora in the Cecum of Nanbu Kashiwa

et al. 2014

View full text Add to dashboard Cite

Since the growth promoter effect of antibiotics was found, many kinds of antibiotics have been used as feed additives for the improvement of growth performance. Since January 1, 2006, however, European Parliament prohibited the use of antibiotics as feed additives for promoting animal growth because of biosecurity threats arising from the increasing resistance of pathogens to antibiotics. Thereafter, various natural substrates having the growth promoter effect have been attempted to use as feed additives instead of antibiotics. Honeybees collect propolis from the cracks in the bark of trees, and it has the versatile pharmacological activities including antibacterial effect. Therefore, the present study aimed to examine the influence of propolis residue, which was the residue after ethanol extraction, on the cecal bacterial flora in the meat-type chicken, Nanbu Kashiwa. Twenty-eight-d-old female Nanbu Kashiwa were given an ordinary diet for 90 days. During last 10 days, an experimental diet including 2% of propolis residue was provided. At the end of experimental period, ceca were removed and DNA was isolated from cecal content. The V3 region of the bacterial 16S rRNA gene was amplified by PCR using the 357F-GC and 518R primers. To indentify the bacterial diversity, denaturing gradient gel electrophoresis (DGGE) was applied, and the optical intensity of DGGE bands was determined. The major bands were excised, and DNA was reamplified by PCR. The sequencing data were analyzed by the Basic Local Alignment Search Tool (BLAST) of DNA Data Bank of Japan. The bacteria identified by PCR-DGGE and BLAST were Lactobacillus aviaries, Lactobacillus, Olsenella, Coriobacterium, Paraprevotella, Prevotellaceae, Clostridiaceae and Bacteroidaceae. The DGGE band of which the optical intensity of the propolis residue group was lower than the control group was the family Prevotellaceae. The successful use of propolis residue to reduce the family Prevotellaceae showed the possibility to use propolis residue as alternative feed additives.

show abstract

“…Dobrowolsky et al (1991) however, observed inhibition zones of 16.0 mm for both S. aureus and for Streptococcus pyogenes. Boyanova et al 2006 used the same methodology to evaluate antimicrobial activity of Bulgarian propolis against anaerobic Gram-positive and Gram-negative bacteria, and recorded inhibition zones greater than or equal to 15 mm for the majority of Gram-positive bacteria, at concentrations of 30 µL of propolis per well.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of propolis-based toothpastes for endodontic treatment

Victorino

Bramante

Watanabe

et al. 2009

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

This study evaluated the antibacterial activity of propolis-based toothpastes used as intracanal medication in endodontic treatment. The propolis-based toothpastes were prepared using an extract established in previous studies (identified as A70D and D70D). Calcium hydroxide paste was used as a control. The diffusion-well method on double-layer agar was used in a culture medium of Tryptic Soy Agar. The plates were kept at room temperature for two hours to allow the diffusion of pastes in the culture medium, and then incubated at 35º C for twenty-four hours in aerobiosis and in microaerophilia (S. mutans). After this period, the total diameter of the inhibition halo was measured. The results were analyzed by ANOVA analysis of variance, followed by the Tukey test at p<0.05. The propolis-based toothpastes presented antibacterial activity against 83.3% of the analyzed bacteria. For 66.7% of these bacteria, the propolis-based toothpastes exhibited greater antibacterial activity than calcium hydroxide. The present results allow us to conclude that the experimental pastes A70D and D70D showed good activity against aerobic bacteria, proving more effective than calcium hydroxide.Uniterms: Propolis/antibacterial activity. Tooth paste/with propolis/antibacterial activity. Endodontics/ treatment.O objetivo deste estudo foi avaliar a atividade antibacteriana de formas farmacêuticas a base de própolis para uso no tratamento endodôntico como medicação intracanal. As formulações de própolis, em forma de pastas, foram preparadas a partir de um extrato pré-estabelecido em estudos anteriores e identificadas como A70D e D70D. Como controle, foi utilizado pasta de hidróxido de cálcio. (26.5 -coagulase negativa) e Staphylococcus epidermidis (6epi). Foi utilizado o método poço difusão em camada dupla de ágar, em meio de cultura Tryptic Soy Agar. As placas foram mantidas à temperatura ambiente por 2 h para permitir a difusão das pastas no meio de cultura, e então incubadas a 35 ºC por 24 h em aerobiose e em microaerofilia (S. mutans). Após este período, foi medido o diâmetro total do halo de inibição. Os resultados foram submetidos ao teste de análise de variância ANOVA seguido do teste de Tukey com p<0,05. As pastas a base de própolis apresentaram atividade antibacteriana contra 83,3% das bactérias analisadas. Para 66,7% das bactérias, as pastas de própolis apresentaram maior atividade

show abstract

In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria

Cited by 47 publications

References 13 publications

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

Influence of Propolis Residue on the Bacterial Flora in the Cecum of Nanbu Kashiwa

Antibacterial activity of propolis-based toothpastes for endodontic treatment

Contact Info

Product

Resources

About