In-vitro affinity of fluoride to proteins, dextans, bacteria and salivary components

Birkeland, Jan Magne; Rölla, Gunnar

doi:10.1016/0003-9969(72)90061-1

Cited by 34 publications

(13 citation statements)

References 11 publications

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“…The initial fluoride retention with 2-min rinses corresponds to pre vious observations on adults with 7.0-ml volumes [Birkeland, 1972], The present findings confirm the suggestion that the increased retention with 10-vs. 7-ml volumes depends mainly upon the initial difference in swallowed fluid [Birkeland, 1972].…”

Section: Discussionsupporting

confidence: 81%

“…(1) The possibility and the degree of errors in the determination of fluoride retention have been discussed previously [Birkeland, 1972]. With the present experimental procedure, only the accuracy of the fluo ride and 51Cr-EDTA measurements may influence deviation in the re covered quantities of these substances.…”

Section: Discussionmentioning

confidence: 99%

“…In the present experiments 7 ml was preferred because: (1) lower fluoride retention was found with 7-than with 10-ml rinses [Birkeland, 1972]; (2) the amount of swallowed fluid was likely to be less with 7 in stead of 10 ml. On the other hand, the 'effective' retention, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…with a fluoride ion activity combination electrode (Orion 96-09) in conjunction with a digital pH/mV meter (Orion 801). From this measurement (in duplicate) and the weight of the expectorate, the amount of fluoride in the fluid was calculated [Birkeland, 1972].…”

Section: Methodsmentioning

confidence: 99%

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The Pharmacokinetics of Fluoride in Mouth Rinses as Indicated by a Reference Substance (<sup>51</sup>Cr-EDTA)

Birkeland

Løkken

1972

Caries Res

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Mouth rinses with 7 ml 0.05% NaF containing ⁵¹Cr-EDTA (2 μCi) were performed by 11 dental staff members. The water-soluble non-absorbable ⁵¹Cr-EDTA was added to enable estimations of the amount of fluid swallowed and the quantity of fluoride retained and/or absorbed in the oral cavity. Experiment 1. 2-min rinses with 7 ml test solution were followed by three 1-min rinses with 10 ml water. The recoveries of ⁵¹Cr-EDTA and fluoride in the first expectorate were 86.3 ± 4.0% and 83.9 ± 4.1% (n = 11), respectively. After the succeeding water rinses, a difference of 1.8 ± 0.9% remained, which represents the fraction of fluoride being absorbed from and/or retained more firmly in the oral cavity. The amount of ⁵¹Cr-EDTA not recovered from the expectorates after this rinsing procedure (5.3 ± 3.0%) indicates the fraction of the test solution swallowed. Experiment 2. This was similar to experiment 1, except that the rinsing with test solution lasted only 30 sec. No significant difference in the total recoveries was observed (0.0 ± 1.0%), and only 1.7 ± 1.6% was estimated to have been swallowed. The findings indicate that (1) negligible parts of the retained fluoride is absorbed from the oral cavity, (2) 2-min vs. 30-sec rinsing periods increased the fraction of fluid swallowed, (3) after 2-min rinses, in contrast to 30-sec rinses, the oral cavity retained a significant fraction of fluoride not removable by the water rinses.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Pharmacokinetics of Fluoride in Mouth Rinses as Indicated by a Reference Substance (<sup>51</sup>Cr-EDTA)

Birkeland

Løkken

1972

Caries Res

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“…Fluoride was recovered from precipi tates formed in ultrafiltrated saliva [Birkeland and Rolla, 1972]. Sedi ments formed in saliva (initially 10~5m fluoride) during 2 days of incubation, were centrifuged at 800 g and re-suspended in saline in the presence or abscence o f 5 % sucrose.…”

Section: Salivamentioning

confidence: 99%

The Effect of pH on the Interaction of Fluoride and Salivary Ions

Birkeland¹

1973

Caries Res

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The interaction between fluoride and some plaque components has indicated that fluoride binds to norganic salivary substances which are ultrafiltrable. The ffect of pH on the affinity of fluoride to these salivary sub stances was investigated in whole saliva, ultrafiltrated saliva, and ‘synthetic saliva’. When the pH was maintained above 7, an initial fluoride ion concentration of 10^-5m (0.2 ppm) decreased to 10^-6M (0.02 ppm). Addition of sucrose to incubated whole saliva or of hydrochloric acid to the inorganic solution prevented the decrease in fluoride ion concentration. In the absence of substances which decreased pH, the fluoride ion concentration decreased from the initial 10^-5M to 10^-6M. This bound fluoride was released when the pH was decreased by means of sucrose or acid. Upon storage of the ‘synthetic saliva’, the calcium concentration decreased from 2 × 10^-3M to 2 × 10^-4M. Fluoride per se seemed to have no measurable effect upon this decrease in calcium concentration. The decrease in fluoride and calcium concentrations was inhibited by citrate. These observations indicate that fluoride in plaque may be bound to a calcium phosphate precipitate, and that the binding is affected by the pH.

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