In vitro and in vivo characterization of an impervious polyester arterial prosthesis: the Gelseal Triaxial® graft

Guidoin, Robert; Marceau, Daniel; Rao, Tian Jian; King, Martin W.; Merhi, Yahye; Roy, Paul‐Emile; Martin, Louisette; Duval, Marie

doi:10.1016/0142-9612(87)90079-2

Cited by 73 publications

(34 citation statements)

References 11 publications

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“…As stated above, NaCl exerts a salting out effect on nonionic surfactants like Triton X405 and, thus, causes an increase of σ. NaCl was dissolved in tha aqueous phase of the emulsion at a concentration of 0.01 M. As expected, very large voids ( Figure 3C) appeared in the scaffold porous structure and the void distribution ( Figure 4C1) presented a maximum shifted slightly toward the high diameter side with respect to that referring to φ0.90 D0. 6 . Comparing the behavior of the cumulative void volumes (Figure 5b1) of the scaffolds obtained with the use of additives, it is evident that φ0.90 D0.6 has the largest percentage of the scaffold volume attributable to voids with D g 40 mµ (77%) or g60 mµ (44%), while for φ0.90 D0.3,S , these percentages are 73 and 32%, respectively.…”

Section: Resultsmentioning

confidence: 95%

Emulsion Templated Scaffolds that Include Gelatin and Glycosaminoglycans

et al. 2008

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Gelatin is one of the most commonly used biopolymer for creating cellular scaffolds due to its innocuous nature. To create stable gelatin scaffolds at physiological temperature (37 degrees C), chemical cross-linking is a necessary step. In a previous paper (Biomacromolecules 2006, 7, 3059-3068), cross-linking was carried out by either radical polymerization of the methacrylated derivative of gelatin (GMA) or through the formation of isopeptide bonds catalyzed by transglutaminase. The method of scaffold production was based on emulsion templating in which an organic phase is dispersed in the form of discrete droplets into a continuous aqueous solution of the biopolymer. Both kinds of scaffolds were tested as culture medium for hepatocytes. It turned out that the enzymatic cross-linked scaffold performed superiorily in this respect, even though it was mechanically less stable than the GMA scaffold. In the present paper, in an attempt to improve the biocompatibility of the GMA-based scaffold, biopolymers present in the extracellular matrix (ECM) were included in scaffold formulation, namely, chondroitin sulfate and hyaluronic acid. These biopolymers were derivatized with methacrylic moieties to undergo radical polymerization together with GMA. The morphology of the scaffolds was tuned to some extent by varying the volume fraction of the internal phase and to a larger extent by inducing a controlled destabilization of the precursor emulsion through the use of additives. In this way, scaffolds with 44% of the void volume attributable to voids with a diameter exceeding 60 microm and with 79% of the interconnect area attributable to interconnects with a diameter exceeding 20 microm in diameter could be successfully synthesized. To test whether the inclusion of ECM components into scaffold formulation resolves in an improvement of their biocompatibility with respect to GMA scaffolds, hepatocytes were seeded on both kinds of scaffolds and cell viability and function assays were carried out and compared.

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Section: Resultsmentioning

confidence: 95%

Emulsion Templated Scaffolds that Include Gelatin and Glycosaminoglycans

et al. 2008

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“…15 Second, the hydrophilic nature of gelatin has been recognized as being an important contributor to the healing process by retaining cellular elements capable of stimulating tissue ingrowth. 16 On the other hand, the slow bioresorption rate and inflamed implantation site observed with albumin-impregnated grafts may reside in the xenogenic and immunogenic nature of albumin and the cross-linking agent used. 4 In fact, glutaraldehyde has been shown to produce strong cross-linking bonds which may delay bioresorption.…”

Section: Discussionmentioning

confidence: 99%

In VivoBiocompatibility and Degradation Studies of Polyhydroxyoctanoate in the Rat: A New Sealant for the Polyester Arterial Prosthesis

et al. 1999

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The present study examined the biocompatibility and degradation properties of poly (beta-hydroxy octanoate) (PHO) as an impregnation substrate on arterial prostheses. PHO-impregnated polyester grafts sterilized by ethylene oxide (EO) or gamma (gamma) radiation, and polyester Dacron(R) prostheses impregnated with fluoropolymer, gelatin, or albumin were implanted subcutaneously in rats for periods ranging from 2 to 180 days. The biocompatibility was assessed by quantifying the alkaline and acid phosphatase secretion while performing histological studies at the tissue/prosthesis interface. The degradation was determined by chemical analysis of the EO and gamma-sterilized PHO after implantation using differential scanning calorimetry (DSC), wide angle x-ray diffraction (WAXD), and size exclusion chromatography (SEC). Alkaline phosphatase activity by the sterilized PHO and by the gelatin and albumin grafts was significantly elevated early after implantation in contrast to that of the Dacron and fluoropolymer grafts that occurred later, at 7 and 5 days, respectively The peak of acid phosphatase activity for all of the grafts occurred between 5 and 10 days postimplantation, with the gamma-sterilized PHO grafts recording the greatest activity. Histological study revealed that the tissue incorporation into the graft wall was earlier and more complete for the Dacron and fluoropolymer grafts after 6 months than for the gelatin and albumin grafts, because the latter induced important inflammatory reactions during the resorption of the cross-linked protein substrates. The EO and gamma-sterilized PHO grafts exhibited a similar healing sequence characterized by the development of a collagenous tissue surrounding the prostheses. However, no infiltration of tissue into the graft wall was observed after 6 months, mainly because of the presence of the PHO. Degradation of the EO and gamma-sterilized PHO occurred preferentially by a hydrolytic mechanism as shown by a 30% molecular weight decrease after 6 months. In conclusion, PHO showed good biocompatibility in terms of enzyme activity and tissue reaction. Degradation was a slow, in vivo process controlled primarily by a random hydrolytic reaction and by a local enzymatic attack by macrophages and giant cells.

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“…It is widely used in the pharmaceutical and medical fields [23]. Although collagen has many advantages [24], the reconstructed collagen-based scaffolds usually suffer from poor physicochemical properties which limit their application [25].…”

Section: Introductionmentioning

confidence: 99%