In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair

Zhu, Lingxin; Yang, Jingwen; Zhang, Jie; Lei, Dongqi; Xiao, Lan; Cheng, Xue; Lin, Ying; Peng, Bin

doi:10.1016/j.actbio.2014.08.014

Cited by 44 publications

(50 citation statements)

References 41 publications

(69 reference statements)

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“…The enhancement of iRoot BP Plus was also significantly higher than that of MTA (18). Another study indicated that iRoot BP Plus exhibited excellent apatite-forming capability in vitro, and the extracts of iRoot BP Plus promoted the adhesion, migration, and attachment of DPSCs; these processes are the key phenomena during the initial stage of dental pulp repair (31). The stronger apatite-forming capability of iRoot BP Plus than MTA may be attributed to the differences in their components, particle sizes, and bioactivity (31).…”

Section: Discussionmentioning

confidence: 93%

Evaluation of a Bioceramic as a Pulp Capping Agent In Vitro and In Vivo

Liu

Wang

Dong

2015

Journal of Endodontics

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Section: Discussionmentioning

confidence: 93%

Evaluation of a Bioceramic as a Pulp Capping Agent In Vitro and In Vivo

Liu

Wang

Dong

2015

Journal of Endodontics

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“…This trend is consistent with that described in previous study, which revealed that MTA and iRoot BP Plus can promote dental pulp cell viability (Zhu et al . ,b). By contrast, the cell viability of osteoblast‐like cells has been reported to be suppressed by iRoot BP Plus to varying degrees (Modareszadeh et al .…”

Section: Discussionmentioning

confidence: 99%

“…, Zhu et al . ,b). Compared with MTA, zirconium oxide and bismuth oxide have been used as radiopacifiers to replace tantalum oxide (Levine et al .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the in vitro biocompatibility of a new fast‐setting ready‐to‐use root filling and repair material

Zhu

Zhang

et al. 2016

Int Endodontic J

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“…In the present study, we also verified that the reparative dentin bridge formed upon application of iRoot BP Plus was slightly thicker than that induced by MTA during the observation period of 28 d. There was a trend toward induction of thicker reparative dentin bridge by iRoot BP Plus compared to MTA. This may be attributed in part to the superior apatite-forming ability of iRoot BP Plus (Zhu, Yang, Zhang, Lei, et al 2014). However, this result needs to be further examined under longer capping periods.…”

Section: Discussionmentioning

confidence: 99%

Promotion of Dental Pulp Cell Migration and Pulp Repair by a Bioceramic Putty Involving FGFR-mediated Signaling Pathways

et al. 2015

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Mineral trioxide aggregate is the currently recommended material of choice for clinical pulp repair despite several disadvantages, including handling inconvenience. Little is known about the signaling mechanisms involved in bioceramic-mediated dental pulp repair-particularly, dental pulp cell (DPC) migration. This study evaluated the effects of iRoot BP Plus, a novel ready-to-use nanoparticulate bioceramic putty, on DPC migration in vitro and pulp repair in vivo, focusing on possible involvement of fibroblast growth factor receptor (FGFR)-related signaling, including mitogen-activated protein kinase and Akt pathways. Treatment with iRoot BP Plus extracts enhanced horizontal and vertical migration of DPCs, which was comparable with the effects induced by mineral trioxide aggregate extracts. The DPCs exposed to iRoot BP Plus extracts demonstrated no evident apoptosis. Importantly, treatment with iRoot BP Plus extracts resulted in rapid activation of FGFR, p38 mitogen-activated protein kinase, extracellular signal-regulated kinase (ERK) 1/2, c-Jun-N-terminal kinase (JNK), and Akt signaling in DPCs. Confocal immunofluorescence staining revealed that iRoot BP Plus stimulated focal adhesion formation and stress fiber assembly in DPCs, in addition to upregulating the expression of focal adhesion molecules, including p-focal adhesion kinase, p-paxillin, and vinculin. Moreover, activation of FGFR, ERK, JNK, and Akt were found to mediate the upregulated expression of focal adhesion molecules, stress fiber assembly, and enhanced DPC migration induced by iRoot BP Plus. Consistent with the in vitro results, we observed induction of homogeneous dentin bridge formation and expression of p-focal adhesion kinase, p-FGFR, p-ERK 1/2, p-JNK, and p-Akt near injury sites by iRoot BP Plus in an in vivo pulp repair model. These data demonstrate that iRoot BP Plus can promote DPC migration and pulp repair involving the FGFR-mediated ERK 1/2, JNK, and Akt pathways. These findings provide valuable insights into the signaling mechanisms underlying nanoparticulate bioceramic-mediated pulp repair.

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In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair

Cited by 44 publications

References 41 publications

Evaluation of a Bioceramic as a Pulp Capping Agent In Vitro and In Vivo

Evaluation of a Bioceramic as a Pulp Capping Agent In Vitro and In Vivo

Evaluation of the in vitro biocompatibility of a new fast‐setting ready‐to‐use root filling and repair material

Promotion of Dental Pulp Cell Migration and Pulp Repair by a Bioceramic Putty Involving FGFR-mediated Signaling Pathways

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