In vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot (Scophthalmus maximus L.) farming

Muñoz-Atienza, Estefanía; Araújo, Carlos; Magadán, Susana; Hernández, Pablo E.; Herranz, Carmen; Santos, Ysabel; Cintas, Luis M.

doi:10.1016/j.fsi.2014.10.007

Cited by 72 publications

(33 citation statements)

References 64 publications

(91 reference statements)

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“…In previous studies, complement activity was undetected in the skin mucus of seabream [20,50], but it was reported in . At gene level, a c3 up-regulation was found after yeast dietary administration in gilthead specimens [51] whilst bathing of turbot with acid lactic probiotics also up-regulated its transcription in several tissues [52]. Taking this into account, our results at protein level of C3 over-expression in skin mucus of seabream after Pdp11 probiotic diet may indicate beneficial effects in the key component C3 in skin mucosal…”

Section: Effects Of Pdp11 And/or Overcrowding Stress On Immune-relatementioning

confidence: 56%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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Section: Effects Of Pdp11 And/or Overcrowding Stress On Immune-relatementioning

confidence: 56%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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“…In the case of fish, the probiotic effect of strains of L. lactis have reported for farmed fish such olive flounder and Siberian sturgeon including interference with pathogens [78] and immunomodulatory effects [78,79], such as the increased phagocytic activity of the mucosal leukocytes [3]. Different strains of Lactobacillus and L. lactis showed the ability to inhibit the adhesion to intestinal mucus and activity against Vibrio species [80,81]. These observations could explain the reduction the DGGE bands related to Vibrio detected in our study from the DGGE patterns of fish receiving the probiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Cordero

Guardiola

Tapia‐Paniagua

et al. 2015

Fish & Shellfish Immunology

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“…The protein concentration of the gut mucus was adjusted to 0.5 mg/ml as determined by the Protein Assay kit (BioRad). Mucus adhesion assays [25] and bacterium (vegetative cell and spore) labeling [26] were conducted according to previous reports with some modifications. Firstly, bacterial pellets (vegetative cells and spores), obtained as described above, were resuspended to 10 8 cfu/ml in 0.1 M Tris (pH 8.0) and incubated with 100 mM 5(6)-carboxyfluorescein diacetate (cFDA, Sigma-Aldrich) at 37 C for 40 min for labeling.…”

Section: Adhesions To Caco-2 Cells and Gut Mucus Of Grass Carpmentioning

confidence: 99%

Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed

Guo

Chen

Kong

et al. 2016

Fish & Shellfish Immunology

117

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In vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot (Scophthalmus maximus L.) farming

Cited by 72 publications

References 64 publications

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed

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