In vitro and in vivo genotoxicity tests on fullerene C60 nanoparticles

Shinohara, Naohide; Matsumoto, Kyomu; Endoh, Shigehisa; Maru, Junko; Nakanishi, Junko

doi:10.1016/j.toxlet.2009.09.012

Cited by 107 publications

(62 citation statements)

References 35 publications

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“…According to this, we considered that our results, shown here using peripheral blood of mice, re‰ected genotoxicity of C60 on bone marrows. Our data are consistent with theˆnding that C 60 administered by gavage to ICR mice is negative in the in vivo bone marrow micronucleus test (4). These reports and our result suggest that intraperitoneal injection and gavage of C60 are negative for genotoxicity on bone marrow cells including erythroid precursors and HSCs.…”

Section: Discussionsupporting

confidence: 93%

“…It was reported that intratracheal instillation of C60 increased both mutation frequency detected by gpt-assay and DNA damage detected by comet assay in lung (3). Nevertheless another group showed that treatment with C60 by gavage has no genotoxical eŠect in ICR mice, using in vivo micronucleus test in bone marrow cells (4). These discrepancies could have been caused by diŠer-ences in administration route, test method, or target organ.…”

Section: Introductionmentioning

confidence: 99%

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Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Horibata

Ukai

Naoki

et al. 2011

Genes and Environment

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Carbon nanoparticles, such as carbon nanotubes and fullerene (C 60 ), are potential candidates as leading substances in nanotechnologicalˆelds, but little is known about their safety. Here we examined in vivo genotoxicity of C 60 , by performing the Pig-A gene mutation assay in the peripheral blood of male C57BL/6Cr mice. Mice were given single intraperitoneal injection of 3 mg of C 60 particles in 0.5 mL suspension containing 0.1%-Tween80-saline. As a positive control for the Pig-A gene mutation assay, mice were given a single oral administration of N-nitroso-Nethylurea. At 2 and 8 weeks after treatments, we analyzed CD24-negative and -positive red blood cells in peripheral blood and calculated Pig-A mutant frequencies. As a result, we detected no signiˆcant diŠerences in the mutant frequencies between C 60 treated and non-treated mice, indicating that C 60 is negative for genotoxicity in vivo in the limited target tissues assessed in this study. For the full assessment, we need comprehensive whole body survey on the genotoxicity of C 60 .

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Horibata

Ukai

Naoki

et al. 2011

Genes and Environment

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“…We also confirmed that 5,000 µg/plate of HPFs dissolved in squalane (200 mg/l C 60 ) and 1,000 µg/ plate of HPFs enwrapped in hydrogenated lecithin, glycine soja (soybean) sterols (6 mg/l C 60 ) were not mutagenic to test strains (Kato et al, 2009a(Kato et al, , 2009b. In addition, stable nano-sized C 60 suspensions in 0.1% CMC-Na aqueous solution did not induce significant positive mutagenic responses in any test strain in the bacterial reverse mutation test, when used at concentrations of up to 1,000 µg/plate (Shinohara et al, 2009). These results indicate that regardless of the solution or dispersion used, fullerenes are not mutagenic.…”

Section: Ames Testmentioning

confidence: 97%

“…Mori et al (2006) indicated that HPFs at dose of 5,000 µg/ml were considered not to have the ability in induce chromosomal aberration. In addition, Shinohara et al (2009) reported that stable nano-sized C 60 suspensions in 0.1% CMC-Na aqueous solutions (100 µg/ml and 200 µg/ml as the highest dose in the test without irradiation and with irradiation) were judged to be negative in the presence and absence of S9 mix with and without irradiation. These results suggest that regardless of the solution or dispersion used, fullerenes do not have the ability to induce chromosom- al aberration.…”

Section: Chromosomal Aberrationsmentioning

confidence: 99%

“…In recent years, toxicity studies have been conducted on fullerenes and their derivatives by using laboratory animals, aquatic organisms, and in vivo and in vitro microorganismal systems. Shinohara et al (2009) showed that C 60 in 0.1% carboxymethylcellulose sodium (CMC-Na) or 0.1% Tween-80 was not genotoxic by performing a bacterial reverse mutation assay, an in vitro chromosome aberration assay, and an in vivo micronucleus assay. Furthermore, the inhalation of the latter fullerene solution does not induce significant inflammation and tissue injury in rats (Fujita et al, 2009), and its intraperitoneal application does not result in peritoneal adhesions, fibrous thickening, or tumor formation in mice (Takagi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity

Aoshima¹,

Yamana²,

Nakamura

et al. 2010

J. Toxicol. Sci.

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-We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe 2+ or Cu 2+ . Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.

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Bioimpact of Carbon Nanomaterials

Djordjević

Injac

Mrdjanović

et al. 2013

Advanced Carbon Materials and Technology

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The unique size-dependent properties of carbon nanomaterials (CNMs)-graphene, nanotubes (CNTs) and fullerenes-make them very attractive for diagnostic and therapeutic application. This chapter presents possible application of CNMs. Graphene with extraordinary chemical and physical properties has already revealed a great number of potential applications such as environmental toxic material removal, drug delivery, tissue engineering, and fl uorescence-based biomolecular sensing. The CNT derivatives have many interesting properties which make them potentially useful in a living system as biosensors, bioelectronic devices based on enzyme-nanotube or antibody-nanotube conjugates, chemotherapeutic agents, hyperthermia therapy and immunotherapy agents, agent in treatment of central nervous system disorders, and tissue engineering agent. Fullerene C 60 derivatives have been used as: drug and gene delivery vectors, magnetic resonance imaging agents, radio protectors, antioxidants, HIV-1 protease inhibitors, antigenotoxic agents, and phototherapy agents. Fullerenols are polyhydroxylated derivatives of fullerene (C 60 (OH)n) with remarkable antioxidant, xenobiotic-protective, radioprotective, nanodrug and endohedral gadolinium carrier properties.

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In vitro and in vivo genotoxicity tests on fullerene C60 nanoparticles

Cited by 107 publications

References 35 publications

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity

Bioimpact of Carbon Nanomaterials

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