In vitro and in vivo toxicity evaluation of cationic PDMAEMA-PCL-PDMAEMA micelles as a carrier of curcumin

Tzankova, Virginia; Gorinova, Cvetelina; Kondeva-Burdina, Magdalena; Simeonova, Rumyana; Philipov, Stanislav; Konstantinov, Spiro; Petrov, Petar; Galabov, Dimitar; Yoncheva, Krassimira

doi:10.1016/j.fct.2016.08.026

Cited by 42 publications

(19 citation statements)

References 33 publications

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“…The release of cytosolic enzymes into the blood is an indicator of tissue lesion; a muscle, liver, heart, kidney or skeleton lesion could signicantly increase the content of these enzymes. 53 However, our studies did not register differences in any of the enzymatic parameters between control and treated groups. In Fig.…”

Section: In Vivo Toxicity Evaluationcontrasting

confidence: 60%

Unique catanionic vesicles as a potential “Nano-Taxi” for drug delivery systems. In vitro and in vivo biocompatibility evaluation

et al. 2017

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Section: In Vivo Toxicity Evaluationcontrasting

confidence: 60%

Unique catanionic vesicles as a potential “Nano-Taxi” for drug delivery systems. In vitro and in vivo biocompatibility evaluation

et al. 2017

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“…This finding has been specifically shown in multiple DMAEMA-containing drug delivery systems. 67,68 Here, MSCs, regardless of species, were significantly more resilient to NP treatments than mTenocytes. This trend was consistent when measuring viability via metabolic activity and DNA content, and suggests that differentiation state is an important parameter to consider when designing siRNA delivery systems and optimizing doses.…”

Section: Discussionmentioning

confidence: 85%

Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

Malcolm¹,

Freeberg²,

Wang³

et al. 2017

Biomacromolecules

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pH-responsive diblock copolymers provide tailorable nanoparticle (NP) architecture and chemistry critical for siRNA delivery. Here, diblock polymers varying in first (corona) and second (core) block molecular weight (Mn), corona/core ratio, and core hydrophobicity (%BMA) were synthesized to determine their effect on siRNA delivery in murine tenocytes (mTenocyte) and murine and human mesenchymal stem cells (mMSC and hMSCs, respectively). NP-mediated siRNA uptake, gene silencing, and cytocompatibility were quantified. Uptake is positively correlated with first block Mn in mTenocytes and hMSCs (p ≤ 0.0005). All NP resulted in significant gene silencing that was positively correlated with % BMA (p < 0.05) in all cell types. Cytocompatibility was reduced in mTenocytes compared to MSCs (p < 0.0001). %BMA was positively correlated with cytocompatibility in MSCs (p < 0.05), suggesting stable NP are more cytocompatible. Overall, this study shows that NP-siRNA cytocompatibility is cell type dependent, and hydrophobicity (%BMA) is the critical diblock copolymer property for efficient gene silencing in musculoskeletal cell types.

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“…2‐( N , N ‐Dimethylaminoethyl) methacrylate (DMAEMA) is a cationic pH‐responsive monomer, which is commonly incorporated into block copolymers and has been utilized in a remarkable range of applications, such as gene delivery vectors, filtration techniques, paints and coatings . Until now, the combination of poly[2‐(dimethylamino)ethyl methacrylate] (PDMAEMA) and aliphatic polyesters such as poly(lactic acid), polycaprolactone and polyhydroxybutyrate has been studied with various block sequences. However, no work has been reported about PDMAEMA‐modified PBS to the best of our knowledge, although PBS is a degradable and biocompatible polyester.…”

Section: Introductionmentioning

confidence: 99%

Preparation of poly(butylene succinate)‐poly[2‐(dimethylamino)ethyl methacrylate] copolymers and their applications as carriers for drug delivery

Zhao

Guo

et al. 2018

Polymer International

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Copolymers of poly[2‐(dimethylamino)ethyl methacrylate]–poly(butylene succinate)–poly[2‐(dimethylamino)ethyl methacrylate] (PDMAEMA–PBS–PDMAEMA, PDBD) were synthesized through a chain‐extension reaction. The thermal properties characterized using differential scanning calorimetry showed that the introduction of PDMAEMA chains slightly decreased the melting temperature of PBS. The water contact angle of PDBD copolymer films with media of various pH decreased with a decrease of pH. This should be ascribed to the conformational transition of PDMAEMA blocks from a compact coil to an expanding shape in accordance with the variation of the pH of the surroundings. The results of dynamic light scattering and scanning electron microscopy revealed that PDBD copolymers could form spherical micelles with small particle size and narrow particle size distribution. Furthermore, drug loading (loading content, ca 10%; encapsulation efficiency, ca 60%) and release experiments were conducted using doxorubicin as a hydrophobic model drug. The results of release experiments of copolymer nanomicelles showed that these micelles had pH‐responsive properties. © 2018 Society of Chemical Industry

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In vitro and in vivo toxicity evaluation of cationic PDMAEMA-PCL-PDMAEMA micelles as a carrier of curcumin

Cited by 42 publications

References 33 publications

Unique catanionic vesicles as a potential “Nano-Taxi” for drug delivery systems. In vitro and in vivo biocompatibility evaluation

Unique catanionic vesicles as a potential “Nano-Taxi” for drug delivery systems. In vitro and in vivo biocompatibility evaluation

Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

Preparation of poly(butylene succinate)‐poly[2‐(dimethylamino)ethyl methacrylate] copolymers and their applications as carriers for drug delivery

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