In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

Abstract

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers Abstract: The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

“…Nevertheless, the current results agree with others that show biocompatibility of GuttaFlow original formulation (17,18). This outcome was expected since GuttaFlow 2 presents basically the same composition, and the modified silver microparticles present a smaller number of atoms in their surface compared to nanoparticles (11,12). A previous investigation had confirmed that these features reduce citotoxicity (25).…”

“…Recently, the sealer formulation was modified and GuttaFlow 2 was introduced. Its composition is basically the same of the original product, but nanosilver particles were replaced by micro-silver particles (11)(12). The evaluation of GuttaFlow new formulation is necessary since the biological effects of silver particles depends on its size and surface that are in contact with tissues (13)(14).…”

Biological and antibacterial properties of a new silver fiber post: In vitro evaluation

“…Although no study has yet assessed the antibacterial activity of a silver fiber post; similarly to our results, many Authors have reported the antimicrobial effects of AgNPs incorporated in different dental materials. Some researchers have introduced nanosilver-gutta-percha, as an attempt to improve the antibacterial effect of gutta-percha (36). Other studies demonstrated the bactericidal activity of composite resins containing silver nanoparticles and the positive effects of AgNPs on MTA antimicrobial activities (32,37).…”

Eficacia antibacteriana de tres selladores endodónticos frente al Enterococcus faecalis

“…Wainsteim et al, en el año 2016, evaluaron la actividad antimicrobiana, frente al Enterococcus faecalis, in vitro, de dos selladores convencionales, entre estos AH Plus determinando la medida del halo inhibitorio de este sellador con un valor de 9,47 mm (14). Los resultados de los estudios de Wainsteim, descritos anteriormente, mostraron halos de inhibición provocados por AH Plus mayores al encontrado por el sellador Topseal, debido a que en dichos estudios se crearon pozos en la superfi cie del agar, de esta manera se obtuvo un contacto directo de los selladores y el microorganismo, mientras que en la presente investigación los selladores estaban contenidos en discos de papel cartulina que se pusieron en contacto con el agar sobre el cual se sembró el Enterococcus faecalis.…”

“…El presente estudio demostró la efi cacia antibacteriana del sellador endodóntico a base de óxido de Zinc y Eugenol (Grossfar) cuyo promedio del halo inhibitorio fue de 8,4 mm; resultados similares a esta investigación se encontraron en otros estudios. Wainstein et al, en el año 2016, demostraron la efi cacia antibacteriana del sellador a base de óxido de zinc y eugenol (Endofi ll) frente al Enterococcus faecalis el cual generó un halo inhibitorio de 8,46 mm, similar al halo inhibitorio formado por Grossfar (sellador a base de OZE) de 8,4 mm, utilizado en esta investigación (14).…”