In Vitro Antibacterial, Antimutagenic and Anti-Influenza Virus Activity of Caffeic Acid Phenethyl Esters

Abstract

We assayed in vitro the biological activities of caffeic acid phenethyl esters that had been enzymatically synthesized from 5-chlorogenic acid and phenethyl alcohol. Caffeic acid phenethyl esters showed antioxidant and hyaluronidase-inhibitory activities similar to those of chlorogenic acid and caffeic acid, and markedly higher antibacterial, antimutagenic, and antiviral activities than chlorogenic acid. The antimicrobial activities of caffeic acid phenylethyl esters against Staphylococcus aureus, Bacillus subtiiis, and Pseudomonas aeruginosa (MIC; 0.22-0.44, 0.44-0.88, and 0.44-0.88 mM, respectively) were higher than those of 5-chlorogenic acid and caffeic acid. 2-Caffeic acid phenylethyl ester showed potent antimutagenic activity against quinoline compounds, aromatic amines, and heterocyclic compounds. In addition, 2-caffeic acid phenylethyl ester at concentrations of less than 35 uM promoted the proliferation of normal human pulmonary fibroblasts (WI-38 cells) in culture, but inhibited the proliferation of virus-transformed fibroblasts (WI-38 VA cells; IC50 of 60 uM). 1and 2-Caffeic acid phenylethyl esters at a concentration of 8.8 uM inhibited the growth of type A influenza virus by 95% and 96%, respectively, and that of type B influenza virus by 92% and 94%, respectively. These results indicate that caffeic acid phenylethyl esters have higher antimutagenic and anti-influenza virus activities than chlorogenic acid and caffeic acid.

Transcriptome Analysis Reveals the Mechanism Underlying the Production of a High Quantity of Chlorogenic Acid in Young Leaves of Lonicera macranthoides Hand.-Mazz

“…These components predominately account for the multiple medicinal effects of L . macranthoides , which include antioxidant [ 3 , 4 , 5 ], detoxification [ 6 ], anti-inflammatory [ 7 ], anti-cancer [ 8 , 9 , 10 , 11 ], anti-cardiovascular disease and pain relief properties [ 12 ]. Moreover, L .…”

Evaluation of the biological activity of sunflower hull extracts

“…It is worth mentioning here that sunflower seeds contain phenolic compounds that are mainly chlorogenic, caffeic and quinic acids with few additional compounds (Spirad and Rao, 1987). Both chlorogenic and caffeic acids are reported to exhibit inhibition of enterobacteria, staphylococcus aureus, Bacillus subtilis and Pseudomnoa aeruginosa and other food borne pathogens (Singer, 2005;Kishimoto et al, 2005;Almeida et al, 2006).…”

Can green synthesized propolis loaded silver nanoparticulate gel enhance wound healing caused by burns?

“…In case of PLSN, the reduction in MIC when compared to AgNP could be attributed to the penetration of propolis loaded silver nanoparticles into the microbial cell which showed combined antibacterial activity associated with AgNPs and propolis.Propolis constituents, principally CAPE have been reported to inhibit bacterial growth by preventing cell division. In addition, propolis has been reported to disorganize the cytoplasm, the cytoplasmic membrane and the cell wall, causing a partial bacteriolysis and inhibition of protein synthesis[38,39]. However, PMSN did not show synergistic activity because physical addition of propolis to AgNP dispersion did not involve loading of propolis onto the AgNP failing its penetration into microbial cell.…”