In-vitro Anticancer Activity of Various Plant Extracts

Abstract

Herbal medicinal plants have been explored for a variety of pharmacological properties but still a large number of phytoconstituents are still unexplored. The manuscript aims to assess the anticancer property of Leucas cephalotes (Roth) Spreng, Acalypha indica L. and Lantana camara L. extracts on MDA-MB-231, A-549, PC-3 and Hep-G2 cell lines. The ethanol extract of the selected plants was explored for anticancer activity by SRB assay. Since L. camara exhibited promising activity, this plant’s successive extraction in diff erent extracts (n-hexane, chloroform, ethanol and hydroalcoholic) was further studied. All the extracts were screened for anticancer activity by SRB assay on MDA-MB-231 and A-549 cell lines. Additionally, the apoptotic assay was carried out on n-hexane and chloroform extracts on A-549 cell lines. GCMS also analyzed both extracts for characterization of the phytoconstituents. Obtained data indicate that n-Hexane and chloroform extracts of L. camara showed promising anticancer activity on MDA-MB-231 and A-549 cell lines. Both extracts also induced apoptosis in A-549 cell line. GC-MS data of L. camara extracts revealed the presence of 25 and 19 compounds in n-hexane and chloroform extract, respectively. The present study shows that L. camara has strong anticancer properties against lung cancer cell lines compared to breast, prostate, and hepatoma cancer cell lines

Effect ofLantana camaraethanolic leaf extract on survival and migration of MDA-MB-231 triple negative breast cancer cell line

“…Ramkumar et al reported cytotoxic properties of L. camara root extract-derived gold nanoparticles on MDA-MB-231 cells (Ramkumar et al, 2017) and in a recent study, El-Din et al showed that methanolic extract of L. camara leaves could exert cytotoxic effect on several cancer cell lines including MCF-7 and MDA-MB-231 breast cancer cells; however, the mechanism of cytotoxicity in breast cancer cell lines was not investigated (El-Din et al, 2022). More recently, Mishra et al reported that extracts of the whole Lantana camara plant harbours cytotoxic effect against triple negative MDA-MB-231 cells although the mechanism of cytotoxicity was unexplored for this cell line (Mishra et al, 2023). It is therefore important to explore the mode of cytotoxic action of Lantana camara leaf extract on breast cancer cell lines, particularly on the triple negative breast cancer cells.…”