In vitro antidiabetic activity of polar and nonpolar solvent extracts from Leucas Aspera (Willd.) link leaves

Abstract

Background:Diabetes mellitus is a chronic illness, and the management of diabetes is a global problem. Successful treatment is required to prevent complications and organ damages. Herbal medicines are having minimal adverse effects when compared to the available synthetic drugs to treat such chronic diseases and disorders.Objective:The present study was aimed to evaluate the antidiabetic and antioxidant activity of polar and nonpolar solvent extracts of Leucas aspera (Willd.) link leaves under in vitro models.Materials and Methods:The in vitro antidiabetic activity of petroleum ether (nonpolar) and ethanol (polar) extracts were evaluated in C2C12 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (cell viability method) and glucose uptake assay. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method used for the evaluation of in vitro antioxidant activity.Results:Both the polar and nonpolar solvent extracts of L. aspera had shown better antioxidant activity compared to standard (IC50 = 18.96 and 19.90 μg/mL, respectively). Petroleum ether extract exhibited better cytotoxic activity in C2C12 cell line compared to ethanol extract (concentration of test drug needed to inhibit cell growth by 50% 110.75 ± 5.5 vs. 415.25 ± 8.0 μg/mL) whereas ethanol extract showed enhanced glucose uptake activity than petroleum ether extract in C2C12 cell line at same concentrations.Conclusion:From our study results, we concluded that L. aspera (Willd.) link leaves had shown better antidiabetic activity and antioxidant activity under in vitro models. Nonpolar solvent extract produced slightly better activity than polar solvent extract. This study warrants further research and experiments on animal models.SUMMARY Petroleum ether extract of Leucas aspera (PELA) exhibited slightly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity compared to ethanol extract of L. aspera (EELA)PELA exhibited better cytotoxic activity in C2C12 cell line compared to EELAEELA had shown enhanced glucose uptake activity than PELA in C2C12 cell line at same concentrationsL. aspera leaf extracts have potential scavenging of DPPH radicals similar to that of ascorbic acidOverall, PELA (nonpolar) produced slightly better antidiabetic activity and antioxidant activity than EELA (polar). Abbreviations Used: DM: Diabetes mellitus, EELA: Ethanol extract of Leucas aspera, L. aspera: Leucas aspera, PELA: Petroleum ether extract of Leucas aspera.

In-vitro and In-silico Inhibitory Action of Methanol Extract of Rhynchosia beddomei Whole Plant on α-Glucosidase and α-Amylase

“…The most prevalent tissue in the body, skeletal muscle, is primarily responsible for the postprandial consumption of glucose [16].…”

Phytochemical analysis, cytotoxic, antioxidant and anti-diabetic activities of the aerial parts of Sorghum halepense

“…The anti-diabetic activities of Achillea santolina (Grover et al, 2002), Adiantum capillus-veneris (Al-Snafi, 2013), Agrimony eupatoria (Al-Snafi, 2015), Aristolochia longa (El Omari et al, 2019), Atractylis gummifera (Bouabid et al, 2019), Eruca sativa (Kishore et al, 2017), Gaultheria trichophylla (Alam and Saqib, 2017), Leucas aspera (Annapandian and Sundaram, 2017), Myrica gale, Rhodiola rosea, Rumex acetosa, Taraxacum officinale (Sekhon-Loodu and Rupasinghe, 2019), Trixis angustifolia (Salazar-Gómez et al, 2019) and Urtica dioica (Ahangarpour et al, 2012) have been reported. um phosphate dibasic, DPPH (2, 2-diphenyl-1-picrylhydrazyl), ABTS•+ (2,2-azinobis (3-ethyl-benzothiazoline)-6-sulfonic acid disodium salt), hydrogen peroxide, amylase, DNS (dinitrosalicylic) acid, sea salts, glucophage, potassium persulfate were of analytical grade.…”