In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

Abstract

BackgroundIn phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.MethodsAntioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2−) and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow’s feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.ResultsIn the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox). The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.ConclusionThe combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

Polyphenols extracted from red grape pomace by a surfactant based method show enhanced collagenase and elastase inhibitory activity

“…Moreover the efficiency of the CGA fractions of both raw extracts was six times higher than that of gallic acid which suggests that the inhibition of these enzymes could be the result of synergistic activity of different polyphenols. This has been observed in a formulation of four combined super fruit extracts ( Ginkgo biloba , Punica granatum , Ficus carica , and Morus alba ) against collagenase …”

In-vitro Evaluation of Antioxidant Activity and Anti-collagenase Activity of Thalassia hempricii as a Potent Ingredients for Anti-Wrinkle Cosmetics

“…Additionally, Antioxidant activities of plants extracts were correlated with their total phenolic content. 29 The characteristics of polyphenols may differ, depend on the solvent used during the extraction of the two solvents. 23 There's can be different mechanism of anti-aging or anti-wrinkle properties on plants extract.…”

Identification of a Collagenase-Inhibiting Flavonoid from Alchemilla vulgaris Using NMR-Based Metabolomics

“…When searching for natural collagenase inhibitors, the first screening generally consists of submitting the potentially active plant extracts to an in vitro collagenase inhibitory assay [23][24][25]. In this study, the 1 H NMR spectrum of each extract was performed simultaneously and the data obtained were combined using multivariate data analysis (orthogonal partial least squares, OPLS) to correlate the 1 H NMR metabolomic profiles of the extracts to their respective percentage of collagenase inhibition.…”