In vitro approach to study the synergistic effects of tobramycin and clarithromycin against Pseudomonas aeruginosa biofilms using prokaryotic or eukaryotic culture media

Thellin, Olivier; Zorzi, Willy; Jolois, Olivier; Elmoualij, Benaïssa; Duysens, Guérin; Cahay, Bernard; Streel, Bruno; Charif, Mounia; Bastin, R; Heinen, Ernst; Quatresooz, Pascale

doi:10.1016/j.ijantimicag.2015.02.010

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…Colvin et al [10] and Thellin et al [38] suggested that there was significant strain-to-strain variability in the contribution of Pel and Psl to mature biofilm structure. A similar interpretation suggested that Pel and Psl can serve a redundant function as a structural scaffold in mature biofilms.…”

Section: Se =Standard Errormentioning

confidence: 99%

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

AL-Sheikhly

Musleh

Al-Mathkhury

2020

eijs

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Pseudomonas aeruginosa produces an extracellular biofilm matrix that consists of nucleic acids, exopolysaccharides, lipid vesicles, and proteins. Alginate, Psl and Pel are three exopolysaccharides that constitute the main components in biofilm matrix, with many biological functions attributed to them, especially concerning the protection of the bacterial cell from antimicrobial agents and immune responses. A total of 25 gentamicin-resistant P. aeruginosa selected isolates were enrolled in this study. Biofilm development was observed in 96% of the isolates. In addition, the present results clarified the presence of pelA and pslA in all the studied isolates. The expression of these genes was very low. Even though all biofilms were affected by gentamicin, the results of fold change showed a wide variation. In conclusion, all P. aeruginosa isolates carried psl and pel regardless of the intensity of the biofilm. A strongly positive correlation with gentamicin minimum inhibitory concentration was noticed. ‫ل‬ ‫الجيني‬ ‫التعبير‬pelA ‫و‬ pslA ‫في‬ ‫الزنجارية‬ ‫ائف‬ ‫الزو‬ ‫الجنتامايسين‬ ‫اجهاد‬ ‫عند‬ ‫حسن‬ ‫الزساق‬ ‫عبد‬ ‫محمود‬ 1 , 2 ‫مصلح‬ ‫نجيب‬ ‫,ليث‬ 2 ‫المذخوري‬ ‫فهد‬ ‫جبار‬ ‫حارث‬ , 3 1 ‫ات،‬ ‫السختبر‬ ‫قدػ‬ ‫ال‬ ‫األمؽر‬ ‫ة‬ ‫دائر‬ ‫اق‬ ‫العر‬ ‫بغداد،‬ ‫الرحة،‬ ‫ة‬ ‫ار‬ ‫وز‬ ‫فشية،‬ 2 ‫اق‬ ‫العر‬ ‫االنبار،‬ ‫االنبار،‬ ‫جامعة‬ ‫العلؽم،‬ ‫كلية‬ ‫الحياة،‬ ‫علؽم‬ ‫قدػ‬ 3 ‫اق‬ ‫العر‬ ‫بغداد،‬ ‫بغداد،‬ ‫جامعة‬ ‫العلؽم،‬ ‫كلية‬ ‫الحياة،‬ ‫علؽم‬ ‫قدػ‬ ‫الخالصه‬ ‫و‬ ‫نؽوية‬ ‫احساض‬ ‫مؼ‬ ‫يتألف‬ ‫خلؽي‬ ‫خارج‬ ‫حياتي‬ ‫غذاء‬ ‫قالب‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫تشتج‬ ‫يد‬ ‫الدكر‬ ‫عديد‬ ‫الخارجي‬ ‫و‬ ‫االلجيشات‬ ‫تعد‬ ‫بروتيشات.‬ ‫و‬ ‫دىشية‬ ‫وحؽيرالت‬ psl ‫و‬ pel ‫في‬ ‫األىػ‬ ‫يد‬ ‫الدكر‬ ‫عديد‬ ‫مؼ‬ ‫ثالثة‬ ‫و‬ ‫الحياتي‬ ‫الغذاء‬ ‫تركيب‬ ‫يزطلع‬ ‫الذي‬ ‫السزادات‬ ‫مؼ‬ ‫ية‬ ‫البكتير‬ ‫الخلية‬ ‫حساية‬ ‫وخرؽصا‬ ‫عديدة‬ ‫حياتية‬ ‫بؽظائف‬ ‫مجسؽعو‬ ‫ما‬ ‫اسة‬ ‫الدر‬ ‫ليذه‬ ‫اختير‬ ‫السشاعي.‬ ‫الجياز‬ ‫و‬ ‫الحياتية‬ 22 ‫السقاومة‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫مؼ‬ ‫لة‬ ‫عز‬ ‫في‬ ‫الحياتي‬ ‫الغذاء‬ ‫تكؽن‬ ‫لؽحظ‬ ‫للجشتامايديؼ.‬ 69 ‫مؼ‬ ‫كل‬ ‫وجؽد‬ ‫الحالية‬ ‫الشتائج‬ ‫أوضحت‬ ‫كسا‬ ‫الت.‬ ‫العز‬ ‫مؼ‬ % pslA ‫و‬ pelA ‫جدا.‬ ‫مشخفزا‬ ‫الجيشيؼ‬ ‫ليذيؼ‬ ‫الجيشي‬ ‫التعبير‬ ‫كان‬ ‫ذلغ‬ ‫على‬ ‫عالوة‬ ‫اسة.‬ ‫الدر‬ ‫الت‬ ‫عز‬ ‫جسيع‬ ‫في‬ ‫قد‬ ‫الجشتامايديؼ‬ ‫مؼ‬ ‫الرغػ‬ ‫على‬ ‫كان‬ ‫الجيشي‬ ‫التعبير‬ ‫في‬ ‫ه‬ ‫تاثير‬ ‫ان‬ ‫اال‬ ‫الت،‬ ‫العز‬ ‫لكل‬ ‫الحياتي‬ ‫الغذاء‬ ‫في‬ ‫اثر‬ ‫كل‬ ‫تستلغ‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫الت‬ ‫عز‬ ‫جسيع‬ ‫ان‬ ‫اسة‬ ‫الدر‬ ‫ىذه‬ ‫مؼ‬ ‫ندتشتج‬ ‫ان‬ ‫السسكؼ‬ ‫مؼ‬ ‫اسعة.‬ ‫و‬ ‫بدرجة‬ ‫متغاير‬ 296 ‫مؼ‬ pslA ‫و‬ pelA ‫التر‬ ‫بيؼ‬ ‫ضعيف‬ ‫تباط‬ ‫ار‬ ‫وجؽد‬ ‫لؽحظ‬ ‫كسا‬ ‫الحياتي.‬ ‫الغذاء‬ ‫قؽة‬ ‫و‬ ‫شدة‬ ‫عؼ‬ ‫الشعر‬ ‫بغض‬ ‫كيز‬ ‫مؼ‬ ‫لكل‬ ‫الجيشي‬ ‫التعبير‬ ‫و‬ ‫للجشتامايديؼ‬ ‫األدنى‬ ‫السثبط‬ pslA ‫و‬ pelA .

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Section: Se =Standard Errormentioning

confidence: 99%

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

AL-Sheikhly

Musleh

Al-Mathkhury

2020

eijs

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“…In daily practice, a combination of intravenous antibiotics is often used to treat exacerbations. Synergistic effect and reducing the risk of antimicrobial resistance are potential factors favouring combination therapy [37][38][39]. On the other hand, more antibiotics are accompanied by a higher risk for toxicity and higher costs.…”

Section: Intravenous Antibioticsmentioning

confidence: 99%

Prevention of drug-related complications in cystic fibrosis

Meer

Touw

Heijerman

2019

Current Opinion in Pulmonary Medicine

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Purpose of reviewDue to continuous development of new drugs and better treatment strategies, survival of patients with cystic fibrosis has changed dramatically. Recently, targeted therapy of cystic fibrosis transmembrane conductance regulator (CFTR) modulators have become available. Despite these promising developments, treatment of this complex multiorgan disease constitutes a high and variable amount of other drugs. Complications of pharmacotherapeutic treatment are, therefore, expected to become more prevalent. This gives cause to review drug-related side effects in this new era in cystic fibrosis treatment. Recent findingsWe will discuss cystic fibrosis-related pharmacotherapies with a focus on indication of treatment, side effects and their complications, drug-drug interactions, and options to monitor and prevent drug-induced toxicity. Many recent publications about pharmacotherapy in cystic fibrosis, focus on antifungal therapy and CFTR modulators. We will give an overview of the most important studies.

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“…However, some traditional and novel approaches used to control biofilms have been proposed in the past decades [ 53 ]. Combinations of tobramycin and clarithromycin have shown reasonable success in clearing P. aeruginosa pulmonary biofilm infections [ 54 ]. Meanwhile, combinations of peptide 1018 and some antibiotics such as ciprofloxacin decreased the concentration of antibiotic required to decrease initial biofilms formation and trigger cell death in mature biofilms.…”

Section: The First Line Of Defense: Bacterial Biofilmsmentioning

confidence: 99%

The Three Bacterial Lines of Defense against Antimicrobial Agents

Zhou

Shi

Huang

et al. 2015

IJMS

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Antimicrobial agents target a range of extra- and/or intracellular loci from cytoplasmic wall to membrane, intracellular enzymes and genetic materials. Meanwhile, many resistance mechanisms employed by bacteria to counter antimicrobial agents have been found and reported in the past decades. Based on their spatially distinct sites of action and distribution of location, antimicrobial resistance mechanisms of bacteria were categorized into three groups, coined the three lines of bacterial defense in this review. The first line of defense is biofilms, which can be formed by most bacteria to overcome the action of antimicrobial agents. In addition, some other bacteria employ the second line of defense, the cell wall, cell membrane, and encased efflux pumps. When antimicrobial agents permeate the first two lines of defense and finally reach the cytoplasm, many bacteria will make use of the third line of defense, including alterations of intracellular materials and gene regulation to protect themselves from harm by bactericides. The presented three lines of defense theory will help us to understand the bacterial resistance mechanisms against antimicrobial agents and design efficient strategies to overcome these resistances.

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In vitro approach to study the synergistic effects of tobramycin and clarithromycin against Pseudomonas aeruginosa biofilms using prokaryotic or eukaryotic culture media

Cited by 10 publications

References 17 publications

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

Prevention of drug-related complications in cystic fibrosis

The Three Bacterial Lines of Defense against Antimicrobial Agents

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