In Vitro Assessment of Cell and Tissue Compatibility

Wolf, Michael; Coleman, Kelly P.; Rankin, Edward A.; Lewerenz, Gregory M.

doi:10.1016/b978-0-12-816137-1.00057-x

Cited by 3 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the ability of viruses to multiply and infect can be measured by using suitable cell lines in artificial systems called in vitro antiviral assessments. It is an effective and safe assay as it gives important information about the tissues and the effect of the tested sample on it as a prior step to preclinical and clinical assessments [26]. In traditional medicine, many hundreds of plants, herbs, and marine herbs were used to treat viral infections.…”

Section: Lc-esi-ms/ms-mrm Analysesmentioning

confidence: 99%

Phytochemical Profiling and Antiviral Activity of Green Sustainable Nanoparticles Derived from Maesa indica (Roxb.) Sweet against Human Coronavirus 229E

Abdelgawad,

El-Hawary,

Abd El-Kader

et al. 2023

Plants

View full text Add to dashboard Cite

Plant secondary metabolites are key components for new, safe and effective drugs. Ethanolic extract of Maesa indica Roxb. Sweet (ME) aerial parts were used for biosynthesis of sustainable green zinc oxide nanoparticles (ZnO NPs) with an average particle size 6.80 ± 1.47 nm and zeta potential −19.7 mV. Both transmission electron microscopy and X-ray diffraction assay confirmed the hexagonal shape of ZnO NPs. Phenolic ingredients in ME were identified using LC-ESI-MS/MS-MRM revealing the identification of chlorogenic acid, gallic acid, caffeic acid, rutin, coumaric acid, vanillin, naringenin, quercetin, ellagic acid, 3.4-dihydroxybenzoic acid, methyl gallate, kaempferol, ferulic acid, syringic acid, and luteolin. The major compound was chlorogenic acid at concentration of 1803.84 μg/g. The antiviral activity of ME, ZnO NPs, and combination of ME with ZnO NPs against coronavirus 229E were investigated. ZnO NPs had superior antiviral effect against coronavirus 229E than ME while their combination showed the highest anti-coronavirus 229E effect, with 50% inhibition concentration (IC50) of 5.23 ± 0.18 µg/mL and 50% cytotoxic concentration (CC50) of 138.49 ± 0.26 µg/mL while the selectivity index (SI) was 26.47. The current study highlighted the possible novel anti-coronavirus 229E activity of green ZnO NPs synthesized from Maesa indica. More studies are needed to further investigate this antiviral activity to be utilized in future biomedical and environmental applications.

show abstract

Section: Lc-esi-ms/ms-mrm Analysesmentioning

confidence: 99%

Phytochemical Profiling and Antiviral Activity of Green Sustainable Nanoparticles Derived from Maesa indica (Roxb.) Sweet against Human Coronavirus 229E

Abdelgawad,

El-Hawary,

Abd El-Kader

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…While in vitro tests are based on simplified models in comparison with the complex physiological environment, they valuably contribute to providing insights into potential in vivo tissue/cellular responses before preclinical and clinical studies [87].…”

Section: Biocompatibility Of Hybrid Scaffolds For Cartilage Tementioning

confidence: 99%

Enhanced Biomechanical Properties of Polyvinyl Alcohol-Based Hybrid Scaffolds for Cartilage Tissue Engineering

et al. 2021

View full text Add to dashboard Cite

Articular cartilage damage is a primary feature of osteoarthritis and other inflammatory joint diseases (i.e., rheumatoid arthritis). Repairing articular cartilage is highly challenging due to its avascular/aneural nature and low cellularity. To induce functional neocartilage formation, the tissue substitute must have mechanical properties which can adapt well to the loading conditions of the joint. Among the various biomaterials which may function as cartilage replacements, polyvinyl alcohol (PVA) hydrogels stand out for their high biocompatibility and tunable mechanical features. This review article describes and discusses the enrichment of PVA with natural materials (i.e., collagen, hyaluronic acid, hydroxyapatite, chitosan, alginate, extracellular matrix) ± synthetic additives (i.e., polyacrylic acid, poly-lactic-co-glycolic acid, poly(ethylene glycol) diacrylate, graphene oxide, bioactive glass) to produce cartilage substitutes with enhanced mechanical performance. PVA-based hybrid scaffolds have been investigated mainly by compression, tensile, friction, stress relaxation and creep tests, demonstrating increased stiffness and friction properties, and with cartilage-like viscoelastic behavior. In vitro and in vivo biocompatibility studies revealed positive outcomes but also many gaps yet to be addressed. Thus, recommendations for future research are proposed in order to prompt further progress in the fabrication of PVA-based hybrid scaffolds which increasingly match the biological and mechanical properties of native cartilage.

show abstract

Post‐Print Processing to Minimize Cytotoxicity of 3D‐Printed Photopolymer Resins for Biomedical Applications

Brooks,

Yadavalli

2024

J of Applied Polymer Sci

View full text Add to dashboard Cite

Light‐based 3D printing techniques, including stereolithography (SLA) and digital light processing (DLP), are of great utility owing to their ability to create complex geometries with high speed, high spatial resolution, and often, optical transparency. These are useful for fabricating custom components and structures for various biomedical applications—scaffolds, implants, microfluidic devices, in vitro cellular experiments, etc. However, a drawback of light‐based methods is that uncured resin monomers and other additives have the potential for cytotoxic effects, adversely impacting cells and tissues. In this study, we investigate a facile method to process light‐based 3D printing to mitigate cytotoxicity. Three optically clear, photocurable resins—a commercially available acrylate‐based resin, BioMed Clear, and an ecoresin derived from soybean oil—are subjected to various post‐treatment protocols. These involve combinations of isopropanol (IPA) washing, UV curing, and water or IPA agitation. The in vitro cytotoxicity and effect on cell proliferation are characterized using two cell lines (myoblasts and fibroblasts). Compared to untreated controls, IPA or water wash, and cleaning with a commercial developer, the results indicate that a short postprint cure with double IPA wash effectively reduces cytotoxicity across all resins, particularly BioMed Clear and ecoresin, maintaining nearly 100% cell viability after 7 days and avoiding material cloudiness or brittleness. The study therefore highlights a low‐cost postprint treatment method applicable to existing commercial resins and 3D printers. The minimization in leachate and cytotoxicity of optically clear 3D‐printed parts can pave the way for broader adoption in resource‐limited settings and safer use in cell studies, microfluidics, and other biomedical applications.

show abstract

In Vitro Assessment of Cell and Tissue Compatibility

Cited by 3 publications

References 37 publications

Phytochemical Profiling and Antiviral Activity of Green Sustainable Nanoparticles Derived from Maesa indica (Roxb.) Sweet against Human Coronavirus 229E

Phytochemical Profiling and Antiviral Activity of Green Sustainable Nanoparticles Derived from Maesa indica (Roxb.) Sweet against Human Coronavirus 229E

Enhanced Biomechanical Properties of Polyvinyl Alcohol-Based Hybrid Scaffolds for Cartilage Tissue Engineering

Post‐Print Processing to Minimize Cytotoxicity of 3D‐Printed Photopolymer Resins for Biomedical Applications

Contact Info

Product

Resources

About