2021
DOI: 10.1016/j.foodchem.2020.127671
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In vitro bioaccessibility and bioavailability of iron from fenugreek, baobab and moringa

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Cited by 34 publications
(21 citation statements)
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“…At the end of digestion, the digestive enzymes were inactivated by boiling and clarified by centrifugation. The supernatant was diluted 1:1 with MEM, and fed to the differentiated Caco‐2 cells grown in six well plates as described previously (Khoja et al., 2020). The ferritin content of Caco‐2 cell lysates was estimated using ELISA kit (Calbiotech, Cat# FR248T, El Cajon, CA, USA) and adjusted to cell protein content.…”
Section: Methodsmentioning
confidence: 99%
“…At the end of digestion, the digestive enzymes were inactivated by boiling and clarified by centrifugation. The supernatant was diluted 1:1 with MEM, and fed to the differentiated Caco‐2 cells grown in six well plates as described previously (Khoja et al., 2020). The ferritin content of Caco‐2 cell lysates was estimated using ELISA kit (Calbiotech, Cat# FR248T, El Cajon, CA, USA) and adjusted to cell protein content.…”
Section: Methodsmentioning
confidence: 99%
“…In order to determine the effect of fructose on iron uptake in Caco-2 cell, fructose 1,6-biphosphate (F16BP) was added to iron and this led to an increase in ferritin formation in Caco-2 cells (Rodriguez-Ramiro et al, 2019). Khoja et al (2021) explored the iron bioavailability in fenugreek, boaba and moringa which have been used for medicinal purposes which could be potential sources of iron. The study used in-vitro digestion followed by iron uptake in Caco-2 cells to evaluate the iron bioavailability from these plant sources.…”
Section: Cell Culturementioning
confidence: 99%
“…Daily supplementation of 5-10 g of Moringa leaf powdered leaf could increase the appetite of children aged <5 y and the body weight by 0.53 kg [9]. Moringa leaf extract contains 9.88% bioaccessible iron, 70.1% insoluble protein, 3.5% glutelin, 2.2% prolamin, and 0.3% globulin [10,11]. Previous studies have reported the biochemical substances of Moringa leaf powder to have low absorption of amino acids because of its anti-nutrient substances (i.e., phytic acid, tannins, and saponins).…”
Section: Introductionmentioning
confidence: 99%