In vitro biocompatibility of poly(vinylidene fluoride–trifluoroethylene)/barium titanate composite using cultures of human periodontal ligament fibroblasts and keratinocytes

Teixeira, Lucas Novaes; Crippa, Grasiele Edilaine; Trabuco, Amanda Cristina; Gimenes, Rossano; Zaghete, M. A.; Palioto, Daniela Bazan; Oliveira, Paulo Tambasco de; Rosa, Adalberto Luiz; Beloti, Márcio Mateus

doi:10.1016/j.actbio.2009.08.024

Cited by 27 publications

(21 citation statements)

References 52 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, we selected the PVDF-TrFE/BT membrane due to its good in vitro biocompatibility and the capacity to induce bone formation in both normal and osteoporotic rats and decrease bone resorption by inhibiting osteoclastogenesis. 8,29,30,31,32,33 In addition, in a previous study, the PVDF-TrFE/BT membrane was successfully combined with osteoblast injections to increase the amount of bone formation in the calvarial defects of normal rats. 11 For bone regeneration based on the use of cells, several tissues have been investigated as cell source; however, the bone marrow still remains the most common source, at least in part, due to its ease of accessibility and multilineage differentiation potential.…”

Section: Discussionmentioning

confidence: 99%

Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

et al. 2019

View full text Add to dashboard Cite

Cell therapy associated with guided bone regeneration (GBR) can be used to treat bone defects under challenging conditions such as osteoporosis. This study aimed to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a poly(vinylidenetrifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on bone repair in osteoporotic rats. Osteoporosis was induced in female rats by bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the osteoporotic condition was characterized after 5 months by microtomographic and morphometric analyses. Calvarial defects were created in osteoporotic rats that immediately received the PVDF-TrFE/BT membrane. After 2 weeks, bone marrow-derived MSCs from healthy rats, characterized by the expression of surface markers using flow cytometry, or phosphate-buffered saline (PBS) (Control) were injected into the defects and bone formation was evaluated 4 weeks post-injection by microtomographic, morphometric, and histological analyses. A reduction in the amount of bone tissue in the femurs of OVX compared with SHAM rats confirmed the osteoporotic condition of the experimental model. More bone formation was observed when the defects were injected with MSCs compared to that with PBS. The modification that we are proposing in this study for the classical GBR approach where cells are locally injected after a membrane implantation may be a promising therapeutic strategy to increase bone formation under osteoporotic condition.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In the same way, Gimenes et al showed that the piezoelectricity produced by membranes made of P(VDF‐TrFE) associated with barium titanate induce bone regeneration in rabbit tibiae, demonstrating the relationship between biological mechanisms and electrical phenomena in the osteogenesis. The in vitro biocompatibility and the biological mechanisms associated with bone formation were also investigated using osteoblastic cells from human alveolar bone and using human periodontal ligament fibroblasts . NR is a biocompatible material and some studies showed its potential in angiogenesis, thus the association with piezoelectric materials might be interesting as a biomaterial for bone defect treatment.…”

Section: Discussionmentioning

confidence: 99%

Subcutaneous tissue reaction and cytotoxicity of polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene blends associated with natural polymers

Marques

Holgado

Simões

et al. 2013

J. Biomed. Mater. Res

View full text Add to dashboard Cite

Cytotoxicity and subcutaneous tissue reaction of innovative blends composed by polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene associated with natural polymers (natural rubber and native starch) forming membranes were evaluated, aiming its applications associated with bone regeneration. Cytotoxicity was evaluated in mouse fibroblasts culture cells (NIH3T3) using trypan blue staining. Tissue response was in vivo evaluated by subcutaneous implantation of materials in rats, taking into account the presence of necrosis and connective tissue capsule around implanted materials after 7, 14, 21, 28, 35, 60, and 100 days of surgery. The pattern of inflammation was evaluated by histomorphometry of the inflammatory cells. Chemical and morphological changes of implanted materials after 60 and 100 days were evaluated by Fourier transform infrared (FTIR) absorption spectroscopy and scanning electron microscopy (SEM) images. Cytotoxicity tests indicated a good tolerance of the cells to the biomaterial. The in vivo tissue response of all studied materials showed normal inflammatory pattern, characterized by a reduction of polymorphonuclear leukocytes and an increase in mononuclear leukocytes over the time (p < 0.05 Kruskal-Wallis). On day 60, microscopic analysis showed regression of the chronic inflammatory process around all materials. FTIR showed no changes in chemical composition of materials due to implantation, whereas SEM demonstrated the delivery of starch in the medium. Therefore, the results of the tests performed in vitro and in vivo show that the innovative blends can further be used as biomaterials.

show abstract

“…10 In the absence of a GTR barrier, epithelial cells rapidly migrate into the wound site along the root surface to form a long junctional epithelium that prevents the formation of a new connective tissue attachment, thus compromising the ultimate success of the regenerative therapy. [11][12][13] Numerous GTR membranes have been introduced, beginning with nonresorbable materials that were associated with a high rate of postoperative complications and also required a second surgical procedure for their removal. 14 Hence, further research is needed to develop biomaterials that have the appropriate properties of biocompatibility and remodeling characteristics to predictably achieve periodontal regeneration.…”

Section: Introductionmentioning

confidence: 99%

Decellularization method influences early remodeling of an allogenic tissue scaffold

Goktas

Matuska

Pierre

et al. 2013

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Extracellular matrix-based biomaterials are currently pursued as an alternative to autologous transplants for the treatment of gingival recession and periodontal disease. These grafts offer improved tissue regeneration without the need for a second operative procedure used in current treatments to remove nonresorbable synthetic biomaterials. However, while decellularization is necessary to minimize the potential immunological impact, it can significantly modify the materials architectural and biochemical properties. By understanding cellular responses, it is possible to more specifically target varying clinical situations. These investigations assess a novel allogenic scaffold derived from the human umbilical vein and determine the effects of two decellularization approaches (osmotic lysis and the surfactant Triton X-100) on the biological and mechanical properties during early remodeling events. Results show Triton X-100 to be significantly more effective at extracting lipids, while the extraction of the scaffolds bulk protein, GAG and DNA similar between the two treatments. Once seeded, scaffolds prepared with osmotic lysis displayed increased cellular proliferation and reduced metabolic activity compared to scaffolds treated with surfactant. Biomechanical properties were largely preserved and similar between the two treatments. These results suggest that by optimizing scaffold processing conditions, biological events associated with remodeling can be modulated to tailor scaffold function for specific clinical applications.

show abstract

In vitro biocompatibility of poly(vinylidene fluoride–trifluoroethylene)/barium titanate composite using cultures of human periodontal ligament fibroblasts and keratinocytes

Cited by 27 publications

References 52 publications

Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

Subcutaneous tissue reaction and cytotoxicity of polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene blends associated with natural polymers

Decellularization method influences early remodeling of an allogenic tissue scaffold

Contact Info

Product

Resources

About