In vitro bulblet production of Lachenalia

Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae

“…At a sucrose concentration of 12%, however, not only was induction frequency reduced, but growth of the corm was significantly inhibited. Although increasing sucrose concentration from 3% to 6% in Lachenalia cultures did not improve bulblet formation, it did increase bulblet size (Slabbert and Niederwieser 1999). Speculation exists as to how elevated carbohydrate levels impact storage organ formation and growth.…”

“…There appear to be three broad categories of plant response to photoperiod: (1) induction of storage organs inhibited by continuous dark, for example, Nerine (Jacobs et al 1992), Lilium (Lian et al 2003) and Fritillaria (Paek and Murthy 2002); (2) induction of storage organs promoted by darkness, for example, Narcissus (Steinitz and Yahel 1982) and Lilium (Niimi and Onozawa 1979;Marinangeli et al 1998); and (3) induction of storage organs at similar frequency in both light (16-h photoperiod) or continuous darkness, for example, Hyacinthus (Kim et al 1981) andLachenalia (Slabbert andNiederwieser 1999).…”

“…Major variables associated with this treatment are the extent of reduction and the duration of exposure . For example, temperatures are effective from 4°C for Hyacinthus (Bach et al 1992), 10°C for Lachenalia (Slabbert and Niederwieser 1999), 15°C for Sparaxis (Hauser and Horn 1991) and 20°C for Gladiolus (Nhut et al 2004). In some species, there is a definitive temperature above which storage organ formation is completely inhibited.…”

Effects of light and cytokinin on in vitro micropropagation and microrhizome production in turmeric (Curcuma longa L.)

“…In order to determine the exact factor(s) of microrhizome induction, the effects of light were examined, as the day length has been reported to play important role in in vitro induction of other storage organs such as tubers (Gopal et al 1998;Grewal et al 1996;Vreugdehil et al 1998) and bulbs (Slabbert and Niederwieser 1999). The tissues with two clonally propagated plantlets about 2 cm height were transferred to NK medium and cultivated under different light conditions described above, DFL condition, continuous dark, continuous light, short day condition and long day condition.…”

Effect of temperature on the growth ofLachenaliacv. Ronina during the bulb preparation phase

“…In contrast, day length has little effect. For Lachenalia the effect of temperature has been studied on long term in vitro storage ofvegetative material , in vivo bulb storage for successful flower forcing (Louw, 1991) and in vitro bulblet formation (Slabbert & Niederwieser, 1999). The effects of temperature on the growth and flowering of Lachenalia during the growing season have not been reported previously (De Hertogh & Le Nard, 1993;Roodbol & Niederwieser, 1998).…”