In vitro Conservation of Campanula sclerophylla Kolak ? Endemic Endangered Species of Western Caucasus

Kolomiets, T. M.; Malyarovskaya, VI; Самарина, Л. С.

doi:10.3329/ptcb.v26i2.30564

Cited by 4 publications

(1 citation statement)

References 3 publications

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“…В течение последних пяти лет в коллекции in vitro сохраняются виды: синеголовник приморский (Eryngium maritimum L.), подснежник Воронова (Galanthus woronowii Losinsk.) и колокольчик твердолистный (Campanula sclero phylla Kolak) (Маляровская и др., 2013; Коломиец и др., 2014а, б; Kolomiets et al, 2016). Однако подбор молеку лярных маркеров, эффективных для генотипирования этих видов, до сих пор не осуществлен.…”

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IRAP-analysis for evaluating the genetic stability of endemic and endangered species of the Western Caucasus flora in the collection in vitro

et al. 2019

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The characterization of genetic diversity is one of the main components of the genetic resources collection and management. Molecular markers are the most effective tool for characterizing and assessing genetic diversity in plant collections. IRAP (inter-retrotransposons amplified polymorphism) markers have proven to be some of the most effective for characterizing and evaluating germplasm, confirming the genetic fidelity of in vitro preserved cultivars and species. In this regard, the aim of this work is to test several IRAP primers to identify genetic polymorphism and study the genetic fidelity of three rare and endemic flora species of the Western Caucasus during in vitro conservation. Approbation of 16 IRAP-primers on the investigated species was carried out for Eryngium maritimum L., Galanthus woronowii Losinsk. and Campanula sclerophylla Kolak. The results made it possible to select the most efficient of them for genetic fidelity analysis of micropropagated plants. Out of 16 IRAP primers 8 amplified PCR products in Eryngium maritimum. In Galanthus woronowii as well 8 of 16 IRAP primers resulted in the amplification with the number of DNA fragments ranging from 2 to 12. In Campanula sclerophylla 9 of 16 IRAP primers amplified 1 to 11 fragments, depending on the marker. The results of the genotyping of regenerants were compared with data on stock in situ plants, which were the source of explants for in vitro conservation. In total, 60 regenerants for each species of the natural flora of the Western Caucasus were involved in the study. The results obtained demonstrated no genetic changes of the regenerants in all the studied species. These results were confirmed using ISSR analysis of an extended sample set of microplants for each species. The results obtained can serve as evidence of a low probability of genetic disorders during in vitro propagation and conservation of the species Eryngium maritimum L., Galanthus woronowii Losinsk. and Campanula sclerophylla Kolak.

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Section: Introductionunclassified

IRAP-analysis for evaluating the genetic stability of endemic and endangered species of the Western Caucasus flora in the collection in vitro

et al. 2019

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In vitro regeneration protocol for endemic Campanula leblebicii Yıldırım

Kartal,

Sevindik,

Pirhan

2024

In Vitro Cell.Dev.Biol.-Plant

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Campanula leblebicii, discovered by Yıldırım, is found only in the village of Ovacık in the Kemalpaşa district of İzmir province, and it is one of the valuable endemic species for Turkey flora. Petiole and leaf explants were cultured on full-strength Murashige and Skoog (MS) medium containing 30.0 g L−1 sucrose; 4.0 g L−1 Gelrite; 0.3 mg L−1 1-naphthaleneacetic acid (NAA); 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 zeatin (ZEA); and 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 6-benzyladenine (BA) for callus initiation. To obtain shoot formation, callus was transferred to full-strength MS medium containing 0.3 mg L−1 NAA; 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 gibberellic acid (GA3); and 0, 0.5, 1.0, 2.0, 3.0 mg L−1 BA. Efficient callus (93.33%) and shoot formation (73.33) were observed from petiole explants cultured on MS medium containing 0.3 mg L−1 NAA and 1.0 mg L−1 BA. Rooting was obtained spontaneously in all shoot initiation medium, but efficient rooting occurred on MS medium supplemented with 0.3 mg L−1 NAA and 1.0 mg L−1 BA. Plants were acclimatized to the pots including sterile peat and perlite (1:1, v/v), and, in total, 12 plants were acclimatized from one genotype.

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Bioactive compound and bioactivity fidelitous micropropagation method of Blumea lacera (Burm. f.) DC.: A large scale production potential

Swaraz¹,

Sumi²,

Sultana³

et al. 2020

Industrial Crops and Products

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In vitro Conservation of Campanula sclerophylla Kolak ? Endemic Endangered Species of Western Caucasus

Cited by 4 publications

References 3 publications

IRAP-analysis for evaluating the genetic stability of endemic and endangered species of the Western Caucasus flora in the collection in vitro

IRAP-analysis for evaluating the genetic stability of endemic and endangered species of the Western Caucasus flora in the collection in vitro

In vitro regeneration protocol for endemic Campanula leblebicii Yıldırım

Bioactive compound and bioactivity fidelitous micropropagation method of Blumea lacera (Burm. f.) DC.: A large scale production potential

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