In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles

Alvarenga, Ivan Caldeira Almeida; Pacheco, Fernanda Ventorim; Silva, Sâmia Torres; Bertolucci, Suzan Kelly Vilela; Pinto, José Eduardo Brasil Pereira

doi:10.1007/s11240-015-0766-7

Cited by 105 publications

(72 citation statements)

References 39 publications

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“…MS basal media (1962) is the most commonly used plant culture media. As presented in Table 4, it has a very high salt content, nevertheless, it is the preferred medium for the growth of several species (Alvarenga et al 2015;Grzegorczyk-Karolak et al 2015;Rahman et al 2015). It has been demonstrated that different MS salt concentrations influence growth in several species (Assis et al 2012;Martins et al 2015;Shekhawat et al 2015;Singh et al 2015).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

432

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

432

206

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“…1). Examples include the decrease in yarrow Achillea millefolium and cucumber seedling Cucumis sativus biomass in re sponse to blue light, as well as reduced rooting in yarrow and Phyllanthus tenellus (Su et al 2014, Alvarenga et al 2015. Often, there is variability among responses to light quality across the plant scale: for example, maximal photochemical efficiency and quantum yield of PSII of leaves growing under blue light was significantly higher than under control light, whilst biomass under blue light was significantly lower than under control light (Su et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Seagrass Halophila ovalis is affected by light quality across different life history stages

Strydom¹,

McMahon²,

Kendrick³

et al. 2017

Mar. Ecol. Prog. Ser.

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Seagrass meadows provide crucial ecosystem services to the coastal zone but are threatened globally. Seagrass loss to date has mainly been attributed to anthropogenic activities that reduce light quantity (amount of photosynthetic photon flux density), such as dredging, flooding and eutrophication. However, light quality (wavelengths of light within the visible spectrum) is also altered by these anthropogenic stressors. This study addressed the effect of light quality changes on seagrasses. Aquarium-based experiments were conducted to determine whether the seagrass Halophila ovalis (R.Br.) Hook f. responds to different light quality treatments. Separate experiments were performed in which adults, seeds or seedlings were subjected to monochromatic light treatments in the blue (peak λ = 451 nm), green (peak λ = 522 nm), yellow (peak λ = 596 nm) and red (peak λ = 673 nm) wavelengths with a control of full-spectrum light (λ = 400 − 700 nm, at 200 µmol photons m −2 s −1). This study is unique in that it measured seagrass responses to light across several plant scales (physiology, productivity, morphology and biomass) as well as across life-history stages (seeds, seedlings, adults and flowering). Adult plants responded differently to seeds and seedlings but were generally consistent with terrestrial angiosperms: blue light decreased below-ground productivity; green light influenced morphology (through increased rhizome internode length); red light enhanced seed germination and survival. The findings indicate that both natural and human-induced changes in light quality could significantly affect seagrass growth and reproduction. As a range of anthropogenic activities are currently contributing to the global losses of seagrasses, this research provides timely information on how light quality influences different seagrass life history stages.

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“…Finally, other substrates such as soil (inside the bottles) could be used as an alternative model of plant growth, leading to new possibilities for investigation. The most recent literature describes in vitro studies of VOCs using SPME techniques leading to difficult quantitative interpretations or using destructive methods such as incubation of the harvested tissue at a non‐biological temperature followed by headspace analysis . The glass chamber set‐up was based on preliminary tests focusing on the size of the cuvette system, the development‐age pattern of the plantlets (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…Conversely, in vitro headspace analysis in connection with abiotic stress studies is usually performed under laboratory conditions. A number of studies described in recent literature have used methods involving plant biological sample destruction such as volatile extraction under air‐dried conditions at 40°C for 72 h, or hydrodistillation performed from cut plant materials . We therefore decided to develop a glass collection chamber that would not harm the plant during its in vitro physiological development or during the VOC sampling procedure.…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies described in recent literature have used methods involving plant biological sample destruction such as volatile extraction under air-dried conditions at 40°C for 72 h, or hydrodistillation performed from cut plant materials. 5,6 We therefore decided to develop a glass collection chamber that would not harm the plant during its in vitro physiological development or during the VOC sampling procedure. Another goal for this innovative in vitro system was to use the plant's clonal regeneration ability, leading to genetically stable propagation.…”

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confidence: 99%

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A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

Durenne

Blondel

Druart

et al. 2018

Phytochemical Analysis

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IntroductionThe dynamic headspace sampling technique using thermal desorption, gas chromatography‐mass spectrometry (TD‐GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs), and experiments performed in sterile and controlled conditions can be useful for VOC metabolism investigations.ObjectiveThe main purpose of this study was to set up a laboratory high‐throughput glass chamber for whole plant volatiles analysis. Brassica napus L. plantlets were tested with the developed system to better understand the relationship between low emission of induced terpene and cadmium (Cd)‐related abiotic stress.MethodologyVOCs emitted by 28‐day‐old Brassica napus L. plantlets cultivated in vitro were trapped with our device using adsorbent cartridges that were desorbed with a thermal desorption unit before cryofocusing with a cooled injection system and programmable temperature vaporising inlet into an HP‐5 ms GC column. Terpene detection and quantitation from chromatogram profiles were acquired using selected ion monitoring (SIM) mode during full scan analysis and mass spectra were obtained with a quadrupole‐type mass spectrometer.ResultsThe new trapping method produced reliable qualitative profiles of oilseed rape VOCs. Typical emissions of monoterpenes (myrcene, limonene) and sesquiterpenes (β‐elemene, (E,E)‐α‐farnesene) were found for the different concentrations tested. One‐way analysis of variance for quantitative results of (E,E)‐α‐farnesene emission rates showed a Cd concentration effect.ConclusionThis inexpensive glass chamber has potential for wide application in laboratory sterile approach and replicated research. Moreover, the non‐invasive dynamic sampling technique could also be used to analyse volatiles under both abiotic and biotic stresses.

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In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles

Cited by 105 publications

References 39 publications

In vitro plant tissue culture: means for production of biological active compounds

In vitro plant tissue culture: means for production of biological active compounds

Seagrass Halophila ovalis is affected by light quality across different life history stages

A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

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