In vitro culture of bovine preantral follicles

Saha, Sukumar; Shimizu, Manabu; Geshi, Masaya; Izaike, Yoshiaki

doi:10.1016/s0378-4320(00)00162-7

Cited by 68 publications

(50 citation statements)

References 40 publications

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“…Here, the oocytes from isolated primary follicles were shown to be able to reassemble functional 'follicle units' in vitro and to support the sustained growth of these reorganised follicles to secondary stages over more than 42 days in vitro. After 7, 11 and 30 days of culture increases in follicle diameter of 7 mm (Nuttinck et al 1993), 19 mm (Itoh & Hoshi 2000) and 35 mm (Saha et al 2000) respectively, have been reported for small bovine follicles. In this cohort of studies, follicle survival and growth depended on the use of feeder layers of somatic cells.…”

Section: Primordial and Primary Follicle Culturementioning

confidence: 96%

The in vitro growth and maturation of follicles

Picton

Harris²,

Muruvi³

et al. 2008

Reproduction

242

190

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The development of technologies to grow oocytes from the most abundant primordial follicles to maturity in vitro holds many attractions for clinical practice, animal production technology and research. The production of fertile oocytes and live offspring has been achieved in mice following the long-term culture of oocytes in primordial follicles from both fresh and cryopreserved ovarian tissue. In contrast, in non-rodent species advances in follicle culture are centred on the growth of isolated preantral follicles. As a functional unit, mammalian preantral follicles are well-suited to culture but primordial and primary follicles do not grow well after isolation from the ovarian stroma. The current challenges for follicle culture are numerous and include: optimisation of culture media and the tailoring of culture environments to match the physiological needs of the cell in vivo; the maintenance of cell-cell communication and signalling during culture; and the evaluation of the epigenetic status, genetic health and fertility of in vitro derived mature oocytes. In large animals and humans, the complete in vitro growth and maturation of oocytes is only likely to be achieved following the development of a multistage strategy that closely mimics the ovary in vivo. In this approach, primordial follicle growth will be initiated in situ by the culture of ovarian cortex. Isolated preantral follicles will then be grown to antral stages before steroidogenic function is induced in the somatic cells. Finally, cytoplasmic and nuclear maturation will be induced in the in vitro derived oocytes with the production of fertile metaphase II gametes.

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Section: Primordial and Primary Follicle Culturementioning

confidence: 96%

The in vitro growth and maturation of follicles

Picton

Harris²,

Muruvi³

et al. 2008

Reproduction

242

190

View full text Add to dashboard Cite

show abstract

“…FSH is known to stimulate follicular growth and maintain the integrity of granulosa cells in sheep (CECCONI et al, 1999) and cattle (SAHA, et al, 2000). FSH treatment was shown to stimulate oocyte growth in bovine pre-antral follicles in-vitro (ITOH et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro

et al. 2012

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“…Other attempts to culture bovine oocyte-granulosa cell complexes originating from early antral follicles on the flat substratum in medium supplemented with PVP allowed to improved efficiency of development to blastocyst stage to 12% (HIRAO et al, 2004). Small preantral follicles (diameter 35 to 100 µm) survived in culture for up to 6 or 7 d, however the quality of oocyte ultrastructure appeared to be unsatisfactory not only after culture but even immediately after follicle isolation (WANDJI et al, 1996;SAHA et al, 2000). We have also found that late preantral and early antral follicles isolated from bovine ovaries can grow and survive in vitro (KĄTSKA and RYŃSKA, 1998;KĄTSKA et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of culture methods on cumulus and oocyte morphology and meiotic competence of bovine oocytes from early antral follicles

Kątska-Książkiewicz

Alm

2005

Arch. Anim. Breed.

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Effect of culture methods on cumulus and oocyte morphology and meiotic competence of bovine oocytes from early antral follicles* AbstractThe objective of the present study was to establish a culture system that would maintain the three-dimensional structure of bovine early antral follicles (EAF) or isolated cumulus-oocyte-granulosa complexes (COCGs) and increase the resulting portion of COCs with normal morphology for subsequent IVM. The morphological quality and meiotic competence of oocytes originating from early antral bovine ovarian follicles (0.2 to 0.7 mm and 0.4 to 0.7 mm diameter) were evaluated following culture in vitro for 14 and 7 d, respectively, and subsequent in vitro maturation. Growth culture modifications included culture in the well of the well (WOW) system; a microdroplet of collagen gel (2 x 5 µl vs. 2 x 400 µl; standard system) and culture of EAF in hanging drops of medium (inverted system). Significantly higher (P<0.01) proportions of COCs with normal morphology (60.4%) were obtained from COCGs compared to EAF (4.8%) grown in the WOW system. Embedding of COCGs in microdrops of collagen gel significantly increased proportion of COCs with normal morphology (63.2%) compared to those embedded in standard volume gels (35.3%). Recovery rate of COCs with normal morphology from cultured EAF was improved both by using microdrops of gel (44%) and by culture in the inverted system (39.3%) over that found for the standard system (8.5%).

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In vitro culture of bovine preantral follicles

Cited by 68 publications

References 40 publications

The in vitro growth and maturation of follicles

The in vitro growth and maturation of follicles

Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro

Effect of culture methods on cumulus and oocyte morphology and meiotic competence of bovine oocytes from early antral follicles

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