In vitro culture of feline infectious peritonitis virus

The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells

“…This has been described in detail elsewhere (Hitchcock et al, 1981). In brief, confluent monolayers of FEL cells (O'Reilly & Whitaker, 1969) were inoculated with 1 ml of infected peritoneal fluid and incubated at 37 °C for 8 days.…”

“…FIP virus is fastidious in its choice of host cells and has until recently (Evermann et al, 1981;Hitchcock et al, 1981;Black, 1980;O'Reilly et al, 1979) defied attempts to grow it in tissue culture. For this reason FIP virus has been extensively studied only in animals and in organ cultures (e.g.…”

“…Pedersen & Boyle, 1980;Ward, 1970;Hoshino & Scott, 1978;Osterhaus et al, 1978). O'Reilly et al (1979) succeeded in culturing FIP virus in monolayer cultures of feline embryonic lung (FEL) cells, and a complete account of the in vitro culture method has been reported (Hitchcock et al, 1981). This paper describes in detail, for the first time, the ultrastructure of FIP virus in cultured FEL cells.…”

Expression of Feline Infectious Peritonitis Coronavirus Antigens on the Surface of Feline Macrophage-like Cells

“…Fcwf ceils and a strain of FIPV adapted to this line of macrophage-like cells were studied since they constitute a reproducible in vitro system. FIPV has been propagated also in other cell lines, however, which have not been qualified as macrophage-like (O'Reilly et al, 1979;Black, 1980;Hitchcock et al, 1981 ;Evermann et al, 1981), and some not even of feline origin (H. E. L. Jacobse-Geels & M. C. Horzinek, unpublished observations). Pedersen (1976) was the first to report growth of FIPV in explanted autochthonous peritoneal cells and Weiss & Scott (1981) demonstrated viral antigen in cultivated buffy coat cells of experimentally infected cats.…”

“…Until recently, in vitro cultivation of FIPV could only be achieved in organ or peritoneal cell cultures (Pedersen, 1976;Hoshino & Scott, 1978). Meanwhile, however, several authors have also described growth of FIPV in continuous cell lines of feline origin (Black, 1980;Hitchcock et al, 1981 ;Evermann et al, 1981 ;Woods, 1982). In this study, we present evidence that the cells of the fcwf line (Pedersen et al, 1981) used for propagating FIPV possess properties of macrophages; in addition, exposure of viral antigen on the surface of infected cells is demonstrated, which is considered relevant for the mechanism of FIP immune pathogenesis.…”