In Vitro Cultures and Volatile Organic Compound Production in Chiliadenus montanus (Vhal.) Brullo

Abstract

Callus and microshoot cultures were established for Chiliadenus montanus (Vhal.) Brullo. (Asteraceae), a medicinal plant known for producing volatile organic compounds (VOCs). Callus induction was achieved successfully by culturing leaf explants on full-strength Murashige and Skoog medium (MS) supplemented with 2.2 µM 2, 4-dichlorophenoxy acetic acid (2,4-D) and 6.9 µM kinetin (Kin). Successful direct shoot regeneration was achieved using nodal explants cultured onto half-strength MS media supplemented with 1.4 μM Gibberellic Acid (GA3) and 4.4 μM 6-Benzylaminopurine (BAP). Indirect microshoots were successfully regenerated using callus cultured on MS media supplemented with 8.8 μM BAP, 2.2 μM Zeatin, and 1.4 μM GA3 followed by culturing on MS media supplemented with 8.8 μM BAP and 0.5 μM naphthalene acetic acid (NAA). Using wild plant aerial parts, callus and microshoots samples, VOCs were extracted successfully using Headspace Solid-Phase Micro-Extraction (HS-SPME) and analyzed by gas chromatography–mass spectrometry (GC-MS). In wild plant extracts, sesquiterpene hydrocarbons were found to be predominant with the following principal components: Alloaromadendrene (11.92%), trans-Cadina-1(6),4-diene (7.54%), and α-caryophyllene (6.77%). The analysis of in vitro microshoots revealed high levels of oxygenated monoterpenes with cis-Myrtanol (16.62%), and β-Cyclocitral (14.3%) as the main components. Callus extract was dominated by monoterpene hydrocarbons and the main compounds identified were (Z)-β-Ocimene (22.27%), p-Cymene (15.13%), and α-pinene (13.78%). In conclusion, an efficient in vitro production system of VOCs in C. montanus was established that can be used in the future for boosting their production without endangering wild plants.

Secondary Metabolites Profiling, Antimicrobial and Cytotoxic Properties of Commiphora gileadensis L. Leaves, Seeds, Callus, and Cell Suspension Extracts

“…However, further quantitative targeted analyses are required to quantify the precise concentration of each secondary metabolite. Finally, C. gileadensis callus and cell suspension cultures established in this study proved to be useful for the production of secondary metabolites, and such systems can be used to boost their production as observed in other plant species [40,41].…”

An integrated approach utilizing raman spectroscopy and chemometrics for authentication and detection of adulteration of agarwood essential oils

“…The 26 components identified are numbered in the figure, which matches the serial numbers of each component in Table 2. (Jerković et al, 2011;Su et al, 2012;Chen et al, 2017;Han et al, 2019;Heidary Jamebozorgi et al, 2019;Ben Hassine et al, 2021;Camele et al, 2021;Geng, 2021;Li et al, 2021;Abu-Darwish et al, 2022;Chen et al, 2022;Costa et al, 2022;Jaradat et al, 2022;Yao et al, 2022).…”

“… RI a , calculated from R T s, in relation to those of a series C 7 -C 40 of n-alkanes. RI b , from the literatures ( Jerković et al, 2011 ; Su et al, 2012 ; Chen et al, 2017 ; Han et al, 2019 ; Heidary Jamebozorgi et al, 2019 ; Ben Hassine et al, 2021 ; Camele et al, 2021 ; Geng, 2021 ; Li et al, 2021 ; Abu-Darwish et al, 2022 ; Chen et al, 2022 ; Costa et al, 2022 ; Jaradat et al, 2022 ; Yao et al, 2022 ). …”