In vitro cytokine stimulation assay for glycolipid biosurfactant from Rhodococcus ruber: role of monocyte adhesion

Abstract

Glycolipid biosurfactant (GLB) from Rhodococcus ruber IEGM 231 was found to stimulate tumor necrosis factor-a (TNF-a), interleukin (IL)

Modulation of Cytokine Secretion and Oxidative Metabolism of Innate Immune Effectors by Rhodococcus Biosurfactant

“…They belong to a family of Toll-like receptors, are mainly expressed on myelomonocytic cells [3], and induce a cascade of infl ammatory reactions. Studying the pro-infl ammatory activity of GLB showed that this agent has a stimulatory effect on the secretion of IL-1β and TNF-α in the monocyte fraction [1,2]. These effects were not observed in the mononuclear cell culture.…”

“…The additional stimulation of cells by LPS has an important role under these conditions. In the absence of costimulatory signals from lymphocytes, GLB stimulates the secretion of IL-1β, TNF-α [1,2], and IL-12 by monocytes. On the one hand, the course and outcome of various infectious diseases depend on the ability of a causative agent, its components, and products to induce the synthesis of IL-12, which potentiates the production of IFN-γ and promotes Th1 polarization of the immune response.…”

“…Our previous studies revealed that the biosurfactant product from R. ruber has a modulatory effect on the production of pro-infl ammatory cytokines IL-1β, IL-6, and TNF-α [1,2].…”

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

“…Also, as suggested by Kuyukina et al ., the biosurfactant glycolipid complex synthesized by R. ruber actinobacteria is nontoxic, causes no stimulation or inhibition of the experimental animal behavioral activity, demonstrates no deaths or body weight loss over a 14‐day observation period, and exhibits no appreciable effect on the proliferative activity of peripheral blood leukocytes. Additionally, in a study conducted by Gein et al ., glycolipid biosurfactant from R. ruber displayed no cytotoxicity against human lymphocytes and therefore could be proposed as a potential immune‐modulating and antitumor agent.…”

Glycolipid biosurfactants: Potential related biomedical and biotechnological applications

“…[12][13][14] Owing their natural origins, they are recognized as low or non-toxic. [15][16][17][18][19][20] Also, they well known by a good biodegradability [21][22][23] encouraging their use as alternatives to synthetic surfactants. So, they have the potential to be used in environmental field as enhancing of hydrocarbons solubility, mobility and biodegradation.…”