In vitro cytotoxicity of Alstonia scholaris (R.Br) bark on Vero and HeLa cell lines

Abstract

Alstonia scholaris R.Br is one of forest plants that have medicinal properties used to treat various diseases. This study aimed to determine the activity of in vitro cytotoxicity of Alstonia scholaris bark against HeLa and Vero cells. Alstonia scholaris bark was macerated with 70% ethanol and fractionated using n-hexane and chloroform to obtain n-hexane, chloroform, and ethanol fraction. All three fractions were tested using MTT assay against HeLa (cervix cancer) and Vero (normal) cells. The results showed that chloroform fraction was the most toxic to HeLa cells with IC50 (125.06 μg/mL) and followed by ethanol fraction (200.07 μg/mL), and n-hexane fractions (238.47 μg mL) respectively. On the other hand, ethanol fraction was the least toxic to Vero cell growth with IC50 (579.93 μg/mL), followed by n-hexane fraction (459.47 μg/mL), and chloroform fraction was the most toxic (396.24 μg/mL). It was suggested that ethanol fraction was the best fraction because it had the least toxicity to a normal cell, but still had toxicity to HeLa cell.

Soursop leaves (Annona muricata L.) endophytic fungi anticancer activity against HeLa cells

“…Different materials and solvents may result in different extracted chemical compounds, affecting cytotoxicity. However, the cytotoxicity against HeLa cancer cells obtained in our study was better than Dewangga, 2015 , Zuraida and Mariya, 2019 . Furthermore, the research from Herlina et al, 2019 , Mardiyaningsih and Ismiyati, 2014 produced a better IC 50 value compared to our study.…”

Determination of Sun Protection Factor Lotion of Pulai Stem Extract (Alstonia scholaris (L.) R.Br)

“…This is due to the amount of solvent used. In this study a solvent ratio of 1:3.5 was used, while in the study (Zuraida dan Mariya, 2019) [5] the extraction of the bark of the pulai used the amount of solvent in a ratio of 1:10. And other factors that affect the yield are plant varieties, plant age, plant maintenance processes, environmental factors where plants grow, harvest processes, and plant processing processes [9].…”

“…Plants that are commonly used are leaves, stems, and bark. The bark of the pulai extract contains flavonoids, alkaloids, saponins, tannins, and terpenoids [3], [5], [6]. The bark of the pulai contains flavonoid compounds that are effective antioxidants.…”