In vitro development of embryoids from punched leaf discs ofCoffea canephora

Pierson, Elisabeth S.; Lammeren, A.A.M. van; Schel, J.H.N.; Staritsky, G.

doi:10.1007/bf01279811

Cited by 44 publications

(19 citation statements)

References 12 publications

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“…Diverse pathways can be observed: in Quercus (EI Maataoui et ai., 1990),. coffee (Pierson et al, 1983) and many other plants (Haccius, 1978; epidermal and sub-epidermal cell clusters. This type of ontogenesis from immature zygotic embryos or somatic embryo structures is an example of direct multicellular embryogenesis and is commonly termed budding or adventive embryogenesis.…”

Section: -Multicellular Originmentioning

confidence: 99%

Histology of Somatic Embryogenesis

Michaux-Ferrière

Schwendiman

1992

Reproductive Biology and Plant Breeding

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Section: -Multicellular Originmentioning

confidence: 99%

Histology of Somatic Embryogenesis

Michaux-Ferrière

Schwendiman

1992

Reproductive Biology and Plant Breeding

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“…En C. arabica, la presencia de alguna auxina es siempre necesaria para la obtención de callo embriogénico (Van Boxtel y Bertouly, 1996;Pierson et al, 1983). Por otro lado, el ambiente gaseoso juega un papel muy importante en el cultivo in vitro.…”

Section: Introductionunclassified

“…In C. arabica, the presence of some auxin is always necessary for obtaining embryogenic callus (Pierson et al, 1983;Van Boxtel and Bertouly, 1996). On the other hand, the gaseous environment plays a very important role in the culture in vitro.…”

Section: Introductionmentioning

confidence: 99%

Medio de cultivo e inhibidores de etileno en la embriogénesis somática de café

López-Gómez

Iracheta-Donjuan

Ojeda-Zacarías

et al. 2017

Remexca

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La combinación de sales, hormonas y el ambiente gaseoso in vitro podrían afectar la expresión de la embriogénesis somática. Se estudió el efecto de la combinación de dos medios de cultivo e inhibidores de etileno sobre la inducción de callo embriogénico de Coffea canephora P., y su capacidad para proliferar y formar embriones. Se establecieron explantes foliares de cinco genotipos de C. canephora P., en dos medios diferentes, el 23A8 [sales de Yasuda con BAP (1.12 mg L-1)]; y la secuencia T1B/T2B consistente en un primer cultivo en T1B [50% de sales de MS con 2iP (2 mg L-1), 2, 4-D (0.5 mg L-1) y AIB (1 mg L-1)]; seguido del T2B [50 % de sales de MS con BAP (4 mg L-1) y 2, 4-D (1 mg L-1)]. Estos medios se suplementaronconAgNO3 (6.8mgL-1)yNa2S2O3(6.3mgL-1). El uso de T1B/T2B propició mayor proliferación de callos (hasta 248 mg), pero bajos porcentajes de callo embriogénico (menores a 29 %) y menor grado de diferenciación que los obtenidos en el medio 23A8 (con 160 mg). En la fase de multiplicación se obtuvieron callos de 1 a 2.5 mg y una tasa de multiplicación de 10 a 24 veces la biomasa inicial. En la de diferenciación, se observó un potencial embriogénico de 25 mil a 137 mil embriones por gramo de callo inoculado. En cada etapa el efecto de los inhibidores de etileno dependió del genotipo en interacción con el medio de cultivo.

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“…The genus Coffee includes at least 64 species grouped into four sections (Carvalho and Monaco, 1969). Commercially, only two out of more than 100 coffee species are cultivated, the C. arabica (Arabica) and C. canephora (Robusta) (Pierson et al, 1983;Juma et al, 1994;Carneiro, 1997). The most important species is C. arabica.…”

Section: Introductionmentioning

confidence: 99%

“…To date, micropropagation techniques are employed to produce a large number of new and true-to-type plants in a relatively short period. It has already proved to be successful for several members of the Rubiacea family (Pierson et al, 1983;Carneiro and Ribeiro, 1989;Haidar, 1993;Ismail et al, 2003;Ebrahim et al, 2007;Almeasary, 2008). However, genotypic difference in shoot forming capacity in relation to the cytokinin level in the medium is not well documented.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Four Coffee Cultivars (Coffea arabica L.) from Yemen through Shoot Tip Culture

Habib

Hussein

et al. 2015

HJSC

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The aim of this study is to optimize in vitro multiplication of Coffea arabica cvs 'Benan',' Burai',' Odayni' and 'Odayni-Bayat'. Seeds germinated on modified quarter MS medium with 1.0 g l −1 activated charcoal gave the highest germination percentage of the four cultivars under study. BA at 8.0 mgl -1 was the most effective concentration with an average of 14 shoots per explant, resulting in better and morphologically superior microshoots in 'Benan' cv. However 'Burai' ,'Odayni' and 'Odayni-Bayat' cultivars, showed the highest number of shoots when they were grown on MS medium supplemented with BA at 4.0, 2.0 and 6.0 mgl -1 respectively. Root formation was 100% in response to the application of IAA, IBA and /or NAA to the culture media for the four cultivars. Half MS supplemented with NAA at 2.0, 1.0 and 3.0 mgl -1 was found to be a suitable medium for root induction in excised micro-shoots, of 'Odayni', 'Burai' and 'Odayni-Bayat' cultivars, respectively. The plantlets were successfully acclimatized in the greenhouse; consequently the survived plants reached 100%.

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In vitro development of embryoids from punched leaf discs ofCoffea canephora

Cited by 44 publications

References 12 publications

Histology of Somatic Embryogenesis

Histology of Somatic Embryogenesis

Medio de cultivo e inhibidores de etileno en la embriogénesis somática de café

Micropropagation of Four Coffee Cultivars (Coffea arabica L.) from Yemen through Shoot Tip Culture

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