In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells

Abstract

The neovascularization of ischemic tissue is a crucial initial step for the functional rehabilitation and wound healing. However, the short of seed cell candidate for the foundation of vascular network is still a big issue. Human adipose tissue derived mesenchymal stem cells (hADSCs), which possess multilineage potential, are capable of adipogenic, osteogenic, and chondrogenic differentiation. We examined whether this kind of stem cells could differentiate into endothelial-like cells and participate in blood vessel formation, and whether they could be used as an ideal cell source for therapeutic angiogenesis in ischemic diseases or vascularization of tissue constructs. The results showed that hADSCs, grown under appropriately induced conditions, displayed characteristics similar to those of vessel endothelium. The differentiated cells expressed endothelial cell markers CD34 and vWF, and had high metabolism of acetylated low-density lipoprotein and prostacyclin. In addition, the induced cells were able to form tube-like structures when cultured on matrigel. Our data indicated that induced hADSCs could exhibit characteristics of endothelial cells. Therefore, these cells, as a source of human endothelial cells, may find many applications in such realms as engineering blood vessels, endothelial cell transplantation for myocardial regeneration, and induction of angiogenesis for treatment of regional ischemia.

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

“…Because the formation of a capillary-like network is the specialized function of endothelial cells [27,30,31] , the RCE cell line established here is indeed an endothelial cell line. This conclusion is coincident with that of human vascular endothelial cells differentiated from human adipose tissue derived mesenchymal stem cells reported by Guan et al [43] . Morphology recovery with 5% BCS-containing MEM medium showed that the morphology of the RCE cells recovered from fibroblastic to polygonal on day 7.…”

Differentiation of canine adipose tissue–derived mesenchymal stem cells towards endothelial progenitor cells

“…6 In the present study, the differentiated ASC-EPCs had increased expression of CD34 and VEGFR2 as well as functional vascular characteristics. Some previous studies 25,26 revealed that human ASCs had a low level of expression of VEGFR2 and could gradually differentiate into endothelial-like cells with increased expression of CD34 and VEGFR2. Therefore, we termed endothelial-differentiated ASCs as ASC-EPCs.…”

Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

“…As hAF-MSCs can give rise to osteogenic, chondrogenic, adipogenic, myogenic, endothelial, hepatic or neurogenic lineages (3) they have been shown to be effective in the treatment of many diseases (4). Due to the endothelial differentiation potential of MSCs, they have received a significant amount of attention as an autologous source for cell-based therapy in restoring endothelial functions and promoting vascularization (5,6).…”