In vitro differentiation potential of a new human osteosarcoma cell line (HOS 58)

Siggelkow, Heide; Niedhart, Christopher; Kurre, Wiebke; Ihbe, Angela; Schulz, Andreas; Atkinson, Michael J.; Hüfner, M.

doi:10.1046/j.1432-0436.1998.6320081.x

Cited by 40 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In U 2 OS and SAOS-2 cells expression of c-MYC, c-JUN, and c-FOS at the protein level was analyzed by FACS, and at the molecular level was analyzed by RT-PCR. High levels for c-MYC were demonstrated also on HOS 58 cells [Siggelkow et al, 1998]. The de®nition of osteosarcoma requires the production of osteoid, which is incompletely mineralized when produced by tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Cellular and molecular properties associated with osteosarcoma cells

Benayahu

Shur

Marom

et al. 2001

J of Cellular Biochemistry

View full text Add to dashboard Cite

Osteosarcoma cells are recognized by abnormal function that causes a primary bone tumor. Osteosarcoma cells U(2)OS and SAOS-2 were analyzed for the expression of cell surface markers. High expression was quantified for hyaloronidase receptor (CD-44) > moderate for integrins (CD-51 and -61), > and lower for selectins (CD-62). High mitotic capacity were demonstrated by gene expression (measured by RT-PCR) and the protein level (measured by FACS) for cFOS, cMYC, and cJUN. The basic definition of osteosarcoma is excessive production of pathological osteoid. Expression of mRNA for matrix genes osteocalcin, osteonectin, and biglycan was studied. Osteocalcin and osteonectin were detected in RNA from primary cultured marrow stromal, trabecular bone cells, and osteosarcoma cell lines (U(2)OS, SAOS-2). mRNA for biglycan was detected only in primary cells and MG-63 cell line and was undetectable in RNA from U(2)OS, SAOS-2 osteosarcoma cell lines and by RNA extracted from bone biopsies of osteosarcoma patients. The absence of biglycan message observed in osteosarcoma samples provides evidence for the alterations in the extra cellular matrix which result with non-mineralized osteoid produced by the osteosarcoma cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular and molecular properties associated with osteosarcoma cells

Benayahu

Shur

Marom

et al. 2001

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Human osteosarcoma cells (HOS 58), provided by Professor Dr A. Schultz (Department of Pathology, University of Giessen, Germany), were used for comparison. 16,17 HOS 58 cells were plated at a density of ‫01ן1‬ 5 cells/ml and cultivated in ISCOVE's medium (Biochrom) supplemented with 10% FCS, 1% glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml) either in the presence or absence of metal particles. Human monocytes were obtained from freshly heparinised blood of healthy donors provided by the blood bank by standard methods, as described elsewhere, using density-gradient centrifugation with Ficoll Hypaque (Biochrom).…”

Section: Methodsmentioning

confidence: 99%

Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro

Heinemann

Lohmann²,

Siggelkow³

et al. 2000

The Journal of Bone and Joint Surgery. British volume

View full text Add to dashboard Cite

P eriprosthetic osteolysis is a major cause of aseptic loosening in artificial joint replacement. It is assumed to occur in conjunction with the activation of macrophages. We have shown in vitro that human osteoblast-like cells, isolated from bone specimens obtained from patients undergoing hip replacement, phagocytose fine particles of titanium alloy (TiAlV).The human osteoblast-like cells were identified immunocytochemically by the presence of bone-specific alkaline phosphatase (BAP). With increasing duration of culture, a variable number of the osteoblastic cells became positive for the macrophage marker CD68, independent of the phagocytosis of particles, with a fine granular cytoplasmic staining which was coexpressed with BAP as revealed by immunodoublestaining. The metal particles were not toxic to the osteoblastic cells since even in culture for up to four weeks massively laden cells were vital and had a characteristic morphology. Cells of the human osteosarcoma cell line (HOS 58) were also able to phagocytose metal particles but had only a low expression of the CD68 antigen. Fluorescence-activated cell scanning confirmed our immunocytochemical results. Additionally, the cells were found to be negative for the major histocompatibility complex-II (MHC-II) which is a marker for macrophages and other antigen-presenting cells. Negative results of histochemical tests for tartrate-resistant acid phosphatase excluded the contamination by osteoclasts or macrophages in culture.Our observations suggest that the osteoblast can either change to a phagocytosing cell or that the phagocytosis is an underestimated property of the osteoblast. The detection of the CD68 antigen is insufficient to prove the monocytic lineage. In order to discriminate between macrophages and osteoblasts additional markers should be used. To our knowledge, this is the first demonstration of cells of an osteoblastic origin which have acquired a mixed phenotype of both osteoblasts and macrophages. Received 17 December 1998; Accepted after revision 21 May 1999Resorption of the surrounding bone is commonly associated with aseptic loosening of artificial joints.1-4 Particles of titanium, a common material used for endoprostheses, are released from the surfaces into the surrounding tissue by corrosion or wear. 3,4 The release of particulate wear debris is generally accepted to be responsible for the recruitment of monocytes/macrophages and the formation of foreign-body granulomas. Phagocytosis of wear particles by macrophages stimulates these cells to produce and secrete inflammatory mediators 5,6 which have been implicated in the stimulation of bone resorption. 7,8The role of osteoblasts in contact with wear particles is poorly understood. We have investigated the behaviour of cultured human osteoblast-like cells when exposed to metal particles in vitro. To exclude the presence of cells of myeloid origin, we also studied markers such as CD68, tartrate-resistant acid phosphatase (TRAP) and major histocompatibility complex II (MHC II). CD68 is used a...

show abstract

“…Moreover, it has been shown that PA can stimulate c-myc, c-fos, and c-jun oncogene expression during cell transformation [Tabib and Bachrach, 1994]. c-myc and c-fos oncogenes display several important cellular functions, and their expression has been related to both cell growth and differentiation in osteoblast-like cells [Kirstein and Baglioni, 1988;Siggelkow et al, 1998;Liang et al, 2003]. To our knowledge, no data have been previously reported concerning the PEMF effects on PA synthesis and oncogene expression in osteblast-like cells; however, some studies show that electromagnetic fields can stimulate both PA metabolism [Byus et al, 1987;Kumlin et al, 1998] and c-myc and c-fos transcription [Wei et al, 1990;Campbell-Beachler et al, 1998] in different cell types.…”

Section: Introductionmentioning

confidence: 99%

Changes in polyamines, c‐myc and c‐fos gene expression in osteoblast‐like cells exposed to pulsed electromagnetic fields

Mattei

Gagliano

Moscheni

et al. 2005

Bioelectromagnetics

View full text Add to dashboard Cite

Pulsed electromagnetic field (PEMF) stimulation promotes the healing of fractures in humans, though its effect is little known. The processes of tissue repair include protein synthesis and cell differentiation. The polyamines (PA) are compounds playing a relevant role in both protein synthesis processes and cell differentiation through c-myc and c-fos gene activation. Since several studies have demonstrated that PEMF acts on embryonic bone cells, human osteoblast-like cells and osteosarcoma TE-85 cell line, in this study we analyzed the effect on cell PAs, proliferation, and c-myc and c-fos gene expression of MG-63 human osteoblast-like cell cultures exposed to a clinically useful PEMF. The cells were grown in medium with 0.5 or 10% fetal calf serum (FCS). c-myc and c-fos gene expressions were determined by RT-PCR. Putrescine (PUT), spermidine (SPD), or spermine (SPM) levels were evaluated by HPLC. [(3)H]-thymidine was added to cultures for DNA analysis. The PEMF increased [(3)H]-thymidine incorporation (P < or = .01), while PUT decreased after treatment (P < or = .01); SPM and SPD were not significantly affected. c-myc was activated after 1 h and downregulated thereafter, while c-fos mRNA levels increased after 0.5 h and then decreased. PUT, SPD, SPM trends, and [(3)H]-thymidine incorporation were significantly related to PEMF treatment. These results indicate that exposure to PEMF exerts biological effects on the intracellular PUT of MG-63 cells and DNA synthesis, influencing the genes encoding c-myc and c-fos gene expression. These observations provide evidence that in vitro PEMF affects the mechanisms involved in cell proliferation and differentiation.

show abstract

In vitro differentiation potential of a new human osteosarcoma cell line (HOS 58)

Cited by 40 publications

References 51 publications

Cellular and molecular properties associated with osteosarcoma cells

Cellular and molecular properties associated with osteosarcoma cells

Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro

Changes in polyamines, c‐myc and c‐fos gene expression in osteoblast‐like cells exposed to pulsed electromagnetic fields

Contact Info

Product

Resources

About