In vitro digestion of sinigrin and glucotropaeolin by single strains of Bifidobacterium and identification of the digestive products

Cheng, Dong‐Bing; Hashimoto, Kei; Uda, Yasushi

doi:10.1016/j.fct.2003.09.008

Cited by 105 publications

(100 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When gnotobiotic rats inoculated with a human digestive strain of Bacteroides thetaiotaomicron were dosed with sinigrin , partial hydrolysis of sinigrin to AITC was observed in the large intestine, in which the concentration of B. thetaiotaomicron was similar to that in the human colon. Cheng et al (2004) have shown that sinigrin and glucotropaeolin are hydrolysed in vitro by three species of bifidobacteria of human origin. However, the hydrolysis generates mostly nitriles because of the acidification of the medium by bifidobacteria (Cheng et al 2004).…”

Section: Involvement Of the Colonic Microflora In Glucosinolate Hydromentioning

confidence: 99%

See 1 more Smart Citation

Effect of cooking brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates

Rungapamestry

Duncan²,

Fuller³

et al. 2007

Proc. Nutr. Soc.

137

102

View full text Add to dashboard Cite

The protective effects of brassica vegetables against cancer may be partly related to their glucosinolate content. Glucosinolates are hydrolysed by plant myrosinase following damage of plant tissue. Isothiocyanates are one of the main groups of metabolites of glucosinolates and are implicated in the preventive effect against cancer. During cooking of brassica the glucosinolate–myrosinase system may be modified as a result of inactivation of plant myrosinase, loss of enzymic cofactors such as epithiospecifier protein, thermal breakdown and/or leaching of glucosinolates and their metabolites or volatilisation of metabolites. Cooking brassica affects the site of release of breakdown products of glucosinolates, which is the upper gastrointestinal tract following consumption of raw brassica containing active plant myrosinase. After consumption of cooked brassica devoid of plant myrosinase glucosinolates are hydrolysed in the colon under the action of the resident microflora. Feeding trials with human subjects have shown that hydrolysis of glucosinolates and absorption of isothiocyanates are greater following ingestion of raw brassica with active plant myrosinase than after consumption of the cooked plant with denatured myrosinase. The digestive fate of glucosinolates may be further influenced by the extent of cell rupture during ingestion, gastrointestinal transit time, meal composition, individual genotype and differences in colonic microflora. These sources of variation may partly explain the weak epidemiological evidence relating consumption of brassica to prevention against cancer. An understanding of the biochemical changes occurring during cooking and ingestion of brassica may help in the design of more robust epidemiological studies to better evaluate the protective effects of brassica against cancer.

show abstract

Section: Involvement Of the Colonic Microflora In Glucosinolate Hydromentioning

confidence: 99%

“…Cheng et al (2004) have shown that sinigrin and glucotropaeolin are hydrolysed in vitro by three species of bifidobacteria of human origin. However, the hydrolysis generates mostly nitriles because of the acidification of the medium by bifidobacteria (Cheng et al 2004).…”

Section: Involvement Of the Colonic Microflora In Glucosinolate Hydromentioning

confidence: 99%

Effect of cooking brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates

Rungapamestry

Duncan²,

Fuller³

et al. 2007

Proc. Nutr. Soc.

137

102

View full text Add to dashboard Cite

show abstract

“…SW620 cells (1x10 6 ) were preseeded into T75 tissue culture flasks for 24 h. The cells were then subjected to various aiTc treatments. The treated cells were trypsinized, washed with PBS and fixed with ice-cold 70% ethanol at -20˚C overnight.…”

Section: Determination Of Cell Cycle Distribution By Flow Cytometrymentioning

confidence: 99%

“…When plant tissue is physically damaged, the glucosidic bond is cleaved by endogenous myrosinase producing various products, including isothiocyanates (iTcs), thiocyanates and nitriles (5). upon human ingestion, the remaining glucosinolates may also be broken down by colonial bacteria (6)(7)(8). among these breakdown products, iTcs draw most of the attention due to their antitumor activities.…”

Section: Introductionmentioning

confidence: 99%

Allyl isothiocyanate induces G2/M arrest in human colorectal adenocarcinoma SW620 cells through down-regulation of Cdc25B and Cdc25C

2010

View full text Add to dashboard Cite

Abstract. allyl isothiocyanate (aiTc) is a constituent of cruciferous vegetables that exhibits antitumor activity. in this study, aiTc was shown to inhibit the proliferation of human metastatic colorectal adenocarcinoma SW620 cells in vitro by inducing cell cycle arrest at the G 2 /M phase. The signaling pathway of aiTc action involved the down-regulation of the pivotal cdc25B and cdc25c protein phosphatases in the treated cells. Quantitative real-time Pcr of aiTc-treated SW620 cells revealed a time-dependent down-regulation of cdc25B and cdc25c mrna levels, which resulted in a decrease in the expression levels of these two proteins. upon prolonged exposure, aiTc induced caspase-mediated apoptosis in SW620 cells. The apoptotic process was evidenced by the activation of initiator caspases (-8 and -9) and effector caspases (-3 and -7), and the cleavage of poly(adP-ribose) polymerase (ParP). The antitumor activity of aiTc was further demonstrated in a SW620 xenograft in vivo. Taken together, the results suggest that aiTc is a potential candidate for future research in chemoprevention and chemotherapy.

show abstract

“…Besides plant-derived myrosinase hydrolysis, bacterial hydrolysis resulting from intestinal microbiota has also been linked to glucosinolate degradation during digestion (22,23,24). For example, the hydrolysis of sinigrin, an aliphatic glucosinolate, can be mediated by the human intestinal bacterium Bacteroides thetaiotaomicron in gnotobiotic mice (25).…”

mentioning

confidence: 99%

Thiouracil-Forming Bacteria Identified and Characterized upon Porcine In Vitro Digestion of Brassicaceae Feed

Kiebooms

Wauters

Bussche

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

In recent years, the frequent detection of the banned thyreostat thiouracil (TU) in livestock urine has been related to endogenous TU formation following digestion of glucosinolate-rich Brassicaceae crops. Recently, it was demonstrated that, upon in vitro digestion of Brassicaceae, fecal bacteria induce TU detection in livestock (porcine livestock > bovines). Therefore, the present study was intended to isolate and identify bacteria involved in this intestinal TU formation upon Brassicaceae digestion and to gain more insight into the underlying mechanism in porcine livestock. Twenty porcine fecal inocula (gilts and multiparous sows) were assessed through static in vitro colonic-digestion simulations with rapeseed. After derivatization and extraction of the fecal suspensions, TU was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS 2 ). On average, lower TU concentrations were observed in fecal colonic simulations in gilts (8.35 ng g ؊1 rapeseed ؎ 3.42 [mean ؎ standard deviation]) than in multiparous sows (52.63 ng g ؊1 ؎ 16.17), which correlates with maturation of the gut microbial population with age. Further exploration of the mechanism showed cell-dependent activity of the microbial conversion and sustained TU-forming activity after subjection of the fecal inoculum to moderate heat over a time span of up to 30 min. Finally, nine TU-producing bacterial species were successfully isolated and identified by a combination of biochemical and molecular techniques as Escherichia coli (n ‫؍‬ 5), Lactobacillus reuteri (n ‫؍‬ 2), Enterococcus faecium (n ‫؍‬ 1), and Salmonella enterica subsp. arizonae (n ‫؍‬ 1). This report demonstrates that endogenous formation of TU is Brassicaceae induced and occurs under colonic conditions most likely through myrosinase-like enzyme activity expressed by different common intestinal bacterial species.

show abstract

In vitro digestion of sinigrin and glucotropaeolin by single strains of Bifidobacterium and identification of the digestive products

Cited by 105 publications

References 21 publications

Effect of cooking brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates

Effect of cooking brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates

Allyl isothiocyanate induces G2/M arrest in human colorectal adenocarcinoma SW620 cells through down-regulation of Cdc25B and Cdc25C

Thiouracil-Forming Bacteria Identified and Characterized upon Porcine In Vitro Digestion of Brassicaceae Feed

Contact Info

Product

Resources

About