In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis

Abstract

The aim of this study was to evaluate the in vitro antimicrobial activity of Brazilian brown propolis as an intracanal medication against Enterococcus faecalis. Thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with E. faecalis for 21 days. The specimens were distributed into six groups according to the medicament used as follows: G1-calcium hydroxide paste; G2-Carbowax 400 (control group); G3-20% brown propolis paste; G4-40% brown propolis paste; G5-20% brown propolis paste + calcium hydroxide paste; and G6-40% brown propolis paste + calcium hydroxide paste. The experimental pastes were placed into the canal lumen and left for 14 days. After each period, irrigation was performed with sterile saline to remove the medicament, and the canals were dried with sterile paper points. The dentin chips were removed from the canals with sequential sterile round burs at low speed and were immediately collected in separate test tubes containing BHI broth. The tubes were incubated at 37°C, and microbial growth was analyzed by spectrophotometry after 15 days. All the experimental medications significantly reduced the number of viable bacteria. The G4 and G5 pastes were more effective than the G1 paste, with 35.8%, 41%, and 21.3% antibacterial activity, respectively. Brazilian brown propolis shows antibacterial capacity against E. faecalis.

Beekeeping in Brazil: A Bibliographic Review

“…It should be stressed that red propolis has been suggested to be the only propolis type derived from a plant from the Leguminosae family (D. ecastaphyllum), rich in isoflavones such as genistein and formononetin. Although flavonoids exhibit pleiotropic activity affecting several different targets, and synergistic effects cannot be ruled out, our results suggest that the isoflavone formononetin is responsible at least partially for the antimicrobial activity of red propolis 20 Bittencourt et al [30] Twenty-nine metabolites were identified along with 34 other metabolites that were classified into the following classes: triterpenoids (12), acetyltriterpenoids (3), sesquiterpenes (6), steroids (4), and hydrocarbons (9). The antioxidant capacity (IC50) ranged from 21.50 to 78.77 μg/mL, whereas the content of total phenolic compounds ranged from 31.88 to 204.30 mg GAE/g of dry weight.…”

“…Conclusions 09 Siqueira et al [19] The red propolis, collected in the region of Brejo Grande-SE, presents characteristics of identity and quality compatible with the parameters established by the Ministry of Agriculture and has proven effective against E. faecalis, being able, with more studies, to become a valuable endodontic treatment 10 Pimenta et al [20] The present study demonstrated that, although medications based on brown propolis with or without calcium hydroxide have limitations inherent to an in vitro study, they are effective against E. faecalis 11 Siqueira et al [21] The results of this study show that, similar to chlorhexidine, red propolis alcoholic extract has good fungistatic and fungicidal activity against most samples of Candida species. This antifungal activity may hold a promise for future applications as an alternative treatment for infections caused by these fungi.…”

Potential Effect of Propolis as an Intracanal Medicament on Root Canal Disinfection and Dentin Microhardness: (An in Vitro Study).

“…aromatic aldehydes and alcohols, fatty acids, stilbenes, and β-steroids. The use of propolis as a root canal medicament have been suggested (1,4,7,8,9,10,11,12,13). The aim of the present study is to test the antibacterial effect of propolis and to compare it with bench mark materials as Ca(OH)2 and CHX.…”

“…Propolis is mainly effective against grampositive bacteria (4). So, its use in endodontic treatment is of great importance especially against Enterococcus faecalis (1,3,12,13).…”

Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances

“…(Dianat et al, 2015) and the intra-canal administration of calcium hydroxide, camphorated paramonochlorophenol-(CMCP), formocresol, tricresol formalin and/or a combination of corticosteroid and antibiotics. Given these limitations and based on the traditional use and relative ease of the isolation of active components, plants may provide potential sources of new drugs for the safe and effective treatment of diseases (Santos et al, 2008;Pimenta et al, 2015).…”