In Vitro Evaluation of Biological Effects on Human Keratinocytes Exposed to 900 MHz Electromagnetic Field

Duranti, Guglielmo; Rossi, Antonello; Rosato, Nicola; Fazio, Giuseppe; Sacerdoti, G.; Rossi, Patrizia A.; Falsaperla, Rosaria; Cannelli, V.; Supino, Rosanna

doi:10.1007/s10669-005-4273-y

Cited by 5 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar negative results have been obtained on rat skin with up to 5 W/kg SAR values [Sanchez et al, 2008]. However, lower SAR EMF emitted by a transverse electromagnetic cell (4-8 mW/kg for 18 h, 900 MHz) significantly reduced the growth of exposed human skin cells and caused an increase in the expression of keratin 1 and involucrin [Duranti et al, 2005]. In addition, a 1 h exposure to GSM radiation (1.3 W/kg, 900 MHz, halfwave dipole) generated altered protein expression in human skin in vivo [Karinen et al, 2008].…”

Section: High-throughput Screening Approaches and Resultssupporting

confidence: 77%

“…This observation can be correlated to lower power efficiency and coupling of EMF to the biological samples in comparison to plane-wave emitting devices. However, other work has shown that such low SAR (40-80 mW/kg, 900 MHz) could significantly influence the physiology of human cells [Duranti et al, 2005].…”

Section: Reverberation Chamber For Bio-electromagnetic Experimentationmentioning

confidence: 96%

See 1 more Smart Citation

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber

Roux

Girard

Paladian

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

We exposed normal human epidermal keratinocytes to short duration, high frequency, and low amplitude electromagnetic fields, similar to that used by mobile phone technologies. We paid particular attention to the control of the characteristics of the electromagnetic environment generated within a mode stirred reverberation chamber (statistical homogeneity and isotropy of the field and SAR distribution). Two non-thermal exposure conditions were tested on the epidermal cells: 10-min exposure with a field amplitude of 8 V/m, and 30 min with 41 V/m. Corresponding specific absorption rates ranged from 2.6 to 73 mW/kg (continuous wave, 900 MHz carrier frequency). We collected RNA from cells subjected to these conditions and used it for a large-scale microarray screening of over 47000 human genes. Under these conditions, exposure of keratinocytes to the electromagnetic field had little effect; only 20 genes displayed significant modulation. The expression ratios were very small (close to 1.5-fold change), and none of them were shared by the two tested conditions. Furthermore, those assayed using polymerase chain reaction did not display significant expression modulation (overall mean of the exposed samples: 1.20 ± 0.18). In conclusion, the data presented here show that cultured keratinocytes are not significantly affected by EMF exposure.

show abstract

Section: High-throughput Screening Approaches and Resultssupporting

confidence: 77%

Section: Reverberation Chamber For Bio-electromagnetic Experimentationmentioning

confidence: 96%

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber

Roux

Girard

Paladian

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…We could quote for example the anechoic chamber, and also Transverse ElectroMagnetic (TEM) cells [3,4]. TEM cells have been recently used as in vivo exposure tools in bioelectromagnetic experiments such as cerebral changes in rats [5].…”

Section: Exposure Setups For Bioelectromagnetic Experiments In High Fmentioning

confidence: 99%

“…Thus, the relation (4) was used for two distinct setups using respectively nine locations (calibration for empty MSRC) and four points (in the presence of the biological incubator). Consequently, it also appeared more convenient to refer indifferently to ↔ E max tot using quantity in (4) defined for N = 9 or N = 4.…”

Section: Definition Of Electromagnetic Validating Criteriamentioning

confidence: 99%

“…Table 5 defines the levels of the incident power P in inside the MSRC. For these three levels, the magnitude of the electric field ↔ E max c was measured according to (4) and [21]. The averaged magnitude of the total electric fieldĒ moy tot (quantity more representative for dosimetric experiments) was also computed to enable an accurate comparison with simulated data.…”

Section: Experimental Dosimetrymentioning

confidence: 99%

See 1 more Smart Citation

Mode Stirred Reverberation Chamber (Msrc): A Large and Efficient Tool to Lead High Frequency Bioelectromagnetic in Vitro Experimentation

Lalléchère¹,

Girard²,

Roux³

et al. 2010

PIER B

View full text Add to dashboard Cite

Abstract-An important aspect of the studies undertaken in bioelectromagnetism relates to the choice of exposure facility. The characteristics of a real electromagnetic environment are far more complex compared to the one plane wave irradiation set-up used in the majority of bioelectromagnetic studies. Moreover, biological requirements should represent the starting point in the design of an in vitro exposure system. Indeed it is important to avoid altering the electromagnetic properties of the exposure system in the presence of biological equipments. Related to these two essential points, this article contributes to show the advantages of a Mode Stirred Reverberation Chamber (MSRC) to guarantee a controlled electromagnetic environment around biological materials for in vitro experimentation. An example of irradiation of in vitro human skin cells cultures is considered to illustrate this paper. In order to show that the biological conditions and the physical requirements for in vitro experiments are checked, two aspects are described. Firstly we achieved the characterization of the electromagnetic field generated around the biological system (both equipments and cultures). Secondly the analysis of the Specific Absorption Rate (SAR) inside the biological medium was evaluated both numerically and experimentally. Initially, the statistical properties of fields inside the MSRC were checked with or without the biological devices in order to verify their electromagnetic transparency with respect to the reverberating properties of the electromagnetic environment (inside MSRC). We checked the good agreement of the experimental electromagnetic power distribution with the theoretical one.The computation of electromagnetic energy absorbed by biological medium (SAR) was based upon Finite Difference in Time Domain (FDTD) technique. A numerical analogy was achieved between the MSRC behavior and a free-space finite sum of random plane waves. Simulations are able to provide both an estimation of SAR distribution inside each biological culture dish and a computation of the coupling effects between dishes. Relying on the previous conclusions, temperature measurements were led to evaluate the experimental SAR levels and its time variations inside the MSRC. Two high-frequency (900 MHz) environments were considered: A 10 minutes exposure with field amplitude inside the biological incubator of 7.87 V/m and 30 minutes with 41 V/m (SAR ranged from 2.6 mW/kg to 73 mW/kg, mean values). Numerical and experimental results prove the ability of MSRC to provide a large and efficient tool to achieve bioelectromagnetic experiments at high frequencies.

show abstract

First‐trimester use of paroxetine and congenital heart defects: A population‐based case‐control study

Bakker

Kerstjens-Frederikse

Buys

et al. 2009

Birth Defects Research

104

View full text Add to dashboard Cite

We exposed normal human epidermal keratinocytes to short duration, high frequency, and low amplitude electromagnetic fields, similar to that used by mobile phone technologies. We paid particular attention to the control of the characteristics of the electromagnetic environment generated within a mode stirred reverberation chamber (statistical homogeneity and isotropy of the field and SAR distribution). Two non‐thermal exposure conditions were tested on the epidermal cells: 10‐min exposure with a field amplitude of 8 V/m, and 30 min with 41 V/m. Corresponding specific absorption rates ranged from 2.6 to 73 mW/kg (continuous wave, 900 MHz carrier frequency). We collected RNA from cells subjected to these conditions and used it for a large‐scale microarray screening of over 47000 human genes. Under these conditions, exposure of keratinocytes to the electromagnetic field had little effect; only 20 genes displayed significant modulation. The expression ratios were very small (close to 1.5‐fold change), and none of them were shared by the two tested conditions. Furthermore, those assayed using polymerase chain reaction did not display significant expression modulation (overall mean of the exposed samples: 1.20 ± 0.18). In conclusion, the data presented here show that cultured keratinocytes are not significantly affected by EMF exposure. Bioelectromagnetics 32:302–311, 2011. © 2010 Wiley‐Liss, Inc.

show abstract

In Vitro Evaluation of Biological Effects on Human Keratinocytes Exposed to 900 MHz Electromagnetic Field

Cited by 5 publications

References 21 publications

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber

Mode Stirred Reverberation Chamber (Msrc): A Large and Efficient Tool to Lead High Frequency Bioelectromagnetic in Vitro Experimentation

First‐trimester use of paroxetine and congenital heart defects: A population‐based case‐control study

Contact Info

Product

Resources

About