In vitro evaluation of modified surface microhardness measurement, focus variation 3D microscopy and contact stylus profilometry to assess enamel surface loss after erosive–abrasive challenges

Gyurkovics, Milán; Baumann, Tommy; Carvalho, Thiago Saads; Assunção, Cristiane Meira; Lussi, Adrian

doi:10.1371/journal.pone.0175027

Cited by 13 publications

(12 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another common method used to measure surface loss is the profilometer 5 , 25 , 26 . However, a recent study showed that, although the different methods usually present different numerical values of surface loss measurements, both the indentation method and the profilometry have shown good correlation and either can be used in surface loss studies 27 .…”

Section: Discussionmentioning

confidence: 99%

Chemical and physical factors of desensitizing and/or anti-erosive toothpastes associated with lower erosive tooth wear

João-Souza

Lussi

Baumann

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Toothpastes have a complex formulation and their different chemical and physical factors will influence their effectiveness against erosive tooth wear (ETW). We, therefore, investigated the effect of different desensitizing and/or anti-erosive toothpastes on initial enamel erosion and abrasion, and analysed how the interplay of their chemical and physical factors influences ETW. Human enamel specimens were submitted to 5 erosion-abrasion cycles using 9 different toothpastes and an artificial saliva group, and enamel surface loss (SL) was calculated. Chemical and physical factors (pH; presence of tin; calcium, phosphate and fluoride concentrations; % weight of solid particles; wettability; and particle size) of the toothpaste slurries were then analysed and associated with the amount of SL in a multivariate model. We observed that all desensitizing and/or anti-erosive toothpastes presented different degrees of SL. Besides pH and fluoride, all other chemical and physical factors were associated with SL. The results of this experiment indicate that enamel SL occurs independent of whether the toothpastes have a desensitizing or anti-erosive claim, and that lower SL is associated with the presence of tin, higher concentration of calcium and phosphate, higher % weight of solid particles, smaller particle size, and lower wettability.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemical and physical factors of desensitizing and/or anti-erosive toothpastes associated with lower erosive tooth wear

João-Souza

Lussi

Baumann

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reproducibility of repeated measurements was also very high with low variations in the range of 0 ± 0.031 μm [Attin et al, 2009]. For the indentation analysis, an accuracy value of 0.18 µm and precision values of 0.01 and 0.08 µm have been reported for repeated measurements (10 measurements) [Gyurkovics et al, 2017]. The procedures of both profilometry as well as indentation analysis as performed in this study were mutually validated by experienced investigators from both university centers involved in order to minimize any methodological bias.…”

Section: Discussionmentioning

confidence: 81%

Comparison of Profilometric and Microindentation Analyses for Determining the Impact of Saliva on the Abrasion of Initially Eroded Enamel

et al. 2018

Self Cite

View full text Add to dashboard Cite

The aim of this in vitro study was to investigate the impact of saliva on the abrasion of eroded enamel using two measuring methods. A total of 80 bovine enamel specimens from 20 bovine incisors were allocated to four experimental groups (n = 20 specimens per group). After baseline surface microhardness (SMH) measurements and profilometry all specimens were subjected to erosion (2 min, 1% citric acid, pH: 3.6, 37°C). SMH was determined again, and the depths of the Knoop indentations were calculated. Thereafter, specimens were incubated in human saliva (group 1 – no incubation/control, group 2 – 0.5 h, group 3 – 1 h, group 4 – 2 h) before toothbrush abrasion was performed. After final SMH measurements and profilometry, indentations were remeasured, and surface loss was calculated. SMH did not return to baseline values regardless of the length of saliva incubation. Further, an irreversible substance loss was observed for all specimens. With the indentation method, significantly (p < 0.05) more substance loss was found for controls (least square means ± standard error of 198 ± 19 nm) than for groups 2–4 (110 ± 10, 114 ± 11, and 105 ± 14 nm). Profilometric assessment showed significantly more substance loss for controls (122 ± 8 nm) than for group 4 (106 ± 5 nm). Intraclass correlation for interrater reliability between measurement methods was low (0.21, CI: 0.1–0.3), indicating poor agreement. Exposure of eroded enamel to saliva for up to 2 h could not re-establish the original SMH. The amount of measured substance loss depended on the measurement method applied.

show abstract

“…Mean enamel loss per tooth from toothbrush abrasion was 0.20 μm (range 0.01–0.31 μm measured from the difference in indentation depths at baseline 1.83 μm (0.07 μm) and after abrasion 1.66 μm (0.06 μm), as described in [20].…”

Section: Resultsmentioning

confidence: 99%

“…4). Studies have reported that the loss of dental hard tissue during the initial stage of erosion ranges from a few nanometers to a few micrometers [20, 23, 24]. When the erosion process reaches advanced stages where the enamel crystals dissolve and surface loss occurs, the reflection measurements (SRI) are less sensitive [14].…”

Section: Discussionmentioning

confidence: 99%

“…Changes in reflection and hardness after abrasion was measured by SRI (TableTop and OptiPen) and by SH. Enamel loss from toothbrushing was measured using the indentation method [20], where six Knoop indentations were made on the enamel surface before the abrasive challenge and their lengths were measured before and after the abrasion. From the difference in length, we were able to calculate the difference in indentation depth.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Analytical strategies for clinical studies on dental erosive wear

Stenhagen

Holme

Tveit³

et al. 2019

BMC Oral Health

View full text Add to dashboard Cite

Background There is a need for analytical techniques for measuring Erosive Tooth Wear (ETW) on natural surfaces in clinical studies. The purpose was to investigate the use of two instruments aimed to assess initial to more advanced stages of ETW. Methods Human premolar enamel samples (2x3mm) ( n = 24), were polished flat and mounted in resin cylinders (4 cylinders, 6 samples in each). Part 1: Baseline analyses by White Light Interferometer (WLI), Surface Reflection Intensity (SRI: TableTop and OptiPen) and Surface Hardness (SH). Erosion (1% citric acid (pH 3.6) for 1, 2, 4, 6, 8, 10 min. SRI and SH analyses after every erosion episode and by WLI after 10 min. New indentations were made and enamel loss; was measured by change in indentation depths from toothbrush abrasion (200 g, 60 strokes, 30 s). Another series of 2 × 5 min erosion (totally15 min and 20 min) was analysed with SH and SRI after each erosion, and by WLI (on samples and impressions of samples) after 20 min. Part 2 investigated WLI performance in the interface where initial erosion increases in severity and substance loss occurs. The samples were repolished. Baseline analyses by WLI, SRI (TableTop and OptiPen) and SH. Four cylinders were etched for 1, 2, 4, 8 min respectively and analysed by SRI, SH on samples, and WLI on samples and impressions). Results Part1: SRI decreased from baseline to ~ 6 min etch and increased slightly after abrasion, the two devices correlated well (ICC 0.98 p < 0.001, Spearmans rs 0.91 p < 0.001). SH decreased nearly linearly to 10 min etch, but increased distinctly after abrasion. Mean enamel loss from abrasion alone was 0.2 μm (change in indentation depths). After 10 min etch, it was 0.27 μm (WLI) and after 20 min etch, it was 2.2 μm measured on samples vs 2.4 μm on impressions of samples (7% higher). Part 2: From baseline to 8 min etch; SRI and SH decreased whereas WLI presented increasing etch depths. Conclusions With some adjustments, the use of SRI and WLI in combination seems to be a promising strategy for monitoring ETW in clinical studies.

show abstract

In vitro evaluation of modified surface microhardness measurement, focus variation 3D microscopy and contact stylus profilometry to assess enamel surface loss after erosive–abrasive challenges

Cited by 13 publications

References 38 publications

Chemical and physical factors of desensitizing and/or anti-erosive toothpastes associated with lower erosive tooth wear

Chemical and physical factors of desensitizing and/or anti-erosive toothpastes associated with lower erosive tooth wear

Comparison of Profilometric and Microindentation Analyses for Determining the Impact of Saliva on the Abrasion of Initially Eroded Enamel

Analytical strategies for clinical studies on dental erosive wear

Contact Info

Product

Resources

About