In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: Cyto-, genotoxicity and oxidative stress

Barillet, Sabrina; Jugan, M.-L.; Laye, Matthew J.; Leconte, Y.; Herlin‐Boime, Nathalie; Reynaud, C.; Carrière, Marie

doi:10.1016/j.toxlet.2010.07.009

Cited by 116 publications

(60 citation statements)

References 37 publications

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“…SiC nanocrystals (NCs) are proven to be favorable biological labels due to their good biocompatibility 8,9 , hemocompatibility 10 and excellent solubility in polar solvents 11 . Moreover, they contain many surface groups that are suitable for further chemical modifications for targeted biomolecules 12 .…”

Section: Introductionmentioning

confidence: 99%

Identification of Luminescence Centers in Molecular-Sized Silicon Carbide Nanocrystals

et al. 2015

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Understanding the fluorescence of complex systems such as small nanocrystals with various surface terminations in solution is still a scientific challenge. Here we show that the combination of advanced time-resolved spectroscopy and ab initio simulations, aided by surface engineering, is able to 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 identify the luminescence centers of such complex systems. Fluorescent water soluble silicon carbide (SiC) nanocrystals have been previously identified as complex molecular systems of silicon, carbon, oxygen and hydrogen held together by covalent bonds that made the identification of their luminescence centers unambiguous. The aqueous solutions of molecular-sized SiC nanocrystals are exceedingly promising candidates to realize bioinert non-perturbative fluorescent nanoparticles for in vivo bioimaging, thus the identification of their luminescent centers is of immediate interest. Here we present identification of two emission centers of this complex system: surface groups involving carbon -oxygen bonds and a defect consisting of silicon -oxygen bonds which becomes the dominant pathway for radiative decay after total reduction of the surface. The identification of these luminescent centers reconciles previous experimental results on the surface and pH dependent emission of SiC nanocrystals and helps design optimized fluorophores and nanosensors for in vivo bioimaging.

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Section: Introductionmentioning

confidence: 99%

Identification of Luminescence Centers in Molecular-Sized Silicon Carbide Nanocrystals

et al. 2015

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“…As NPs can be translocated from the lung into the blood, they can move to other organs and tissues (Donaldson et al, 2001;Nemmar et al, 2001), and may generate ROS at these sites. The ROS are highly reactive molecules that can disturb the homeostasis of the intracellular milieu and cause breakdown of membrane lipids, and cause DNA damage (Barillet et al, 2010;Oberdörster et al, 2005;Oberdörster et al, 2007;Papageorgiou et al, 2007). Similarly, recent in vitro studies demonstrated that NPs induce oxidative stress and inflammatory responses leading to genotoxicity and cytotoxicity in cells Asare et al, 2012;Kumar et al, 2011;Patil et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

124

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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“…Silicon carbide (SiC)-nanoparticle (NP) exposure cause reactive oxygen species production, glutathione depletion and inactivation of some antioxidant enzymes: glutathione reductase, superoxide dismutase, but not catalase [14]. Active and native bentonite particles (BPs) could induce significantly the oxidative stress on human B lymphoblast cells in vitro.…”

Section: Recent In Vitro Assays For Oxidative Dna Damages With Industmentioning

confidence: 99%

Oxidative DNA damages by chemical exposures at work

Rim¹

2012

ABB

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ABSTRACTdiseases. These include cancer, Parkinson's disease, Alzheimer's disease, atherosclerosis, heart failure, myocardial infarction, Schizophrenia, bipolar disorder, fragile X syndrome, sickle cell disease and chronic fatigue syndrome. Oxidative stress is likely to be involved in agerelated development of cancer. The reactive species produced in oxidative stress can cause direct damage to the DNA and are therefore mutagenic, and it may also suppress apoptosis and promote proliferation, invasiveness and metastasis [1].Oxidative DNA damage is an inevitable consequence of cellular metabolism, with a propensity for increased levels following toxic insult. Of the molecules subject to oxidative modification, DNA has received the greatest attention, with biomarkers of exposure and effect closest to validation. There are many chemicals in workplaces that could cause oxidative DNA damages such as carcinogens. This review concentrated on studies published between the years 2000 and 2012 that used to detect 8-oxodG in humans (workers), laboratory animals and in cell lines. Given the recent toxicological results from oxidative stress, it is important to review these studies to improve the current understanding of the oxidative DNA damages by chemical exposures at work. It also suggests that biomarkers may be responsible for understanding the role of oxidative DNA damage in disease, highlighting the need for further studies.

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In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: Cyto-, genotoxicity and oxidative stress

Cited by 116 publications

References 37 publications

Identification of Luminescence Centers in Molecular-Sized Silicon Carbide Nanocrystals

Identification of Luminescence Centers in Molecular-Sized Silicon Carbide Nanocrystals

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Oxidative DNA damages by chemical exposures at work

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