In vitro Evaluation of the Cytotoxicity of Different Root Canal Filling

Abstract

Objective:Aim of the present study was to evaluate the cytotoxicity of Real Seal 1 compared to other commercially available endodontic filling materials: Real Seal (SybronEndo, Orange, CA, USA) and Thermafil (Tulsa Dental, Tulsa, OK, USA).Material and Methods:Periodontal ligament cells from healthy patients were cultured. The eluate of Real Seal 1TM (RS1), Real Seal (RS) and Thermafil (TF) samples was used for the cells viability tests, both diluted (50%) or undiluted (100%). Incubation of the specimens was performed in culture medium for 24 h, 48 h and 72 h at 37 °C under sterile conditions. The cellular mortality was evaluated by MTT test. Results were statistically analysed and the statistical significance was set at p< 0.05.Results:None of the studied materials showed toxic effects during the period of observation (0 -72 h) when compared to the control group. Only RS induced a very modest increase in cell mortality (about 3% at both concentrations used, during the first 24 hours), when increasing the incubation time, however, only the lower concentration continued to show modest toxicity.Conclusions:Results of the present study showed that all tested materials did not exhibit cytotoxic effects when compared to the control group.

Differentiation of Dental Pulp Stem Cells on Gutta-Percha Scaffolds

“…Leakage of ZnO nanoparticles was suspected as an early source of cytotoxicity which became apparent after 24 h incubation of fourteen Gutta-percha materials commercially available prior to 1990 [ 28 ]. More recently though, Gambarini et al showed no cytotoxicity up to 72 h to periodontal ligament cells of newer root canal filling materials [ 29 ]. No study has addressed as yet the influence of Gutta-percha on stem cells differentiation.…”

Carrier-based Root Canal Filling Materials: A Literature Review

“…Both materials, RealSeal 1 and Thermafil, were shown to be nontoxic. 60 Different studies compared the sealing ability of these two different carrier-based filling materials with different methods. With the use of fluid filtration system device, it has been shown that RealSeal 1 has significantly better sealing ability at 24 hours than Thermafil.…”