In vitro evaluation of the sealing ability of three newly developed root canal sealers: A bacterial microleakage study

Abstract

BackgroundThe purpose of this study was to compare the sealing ability of MTA Fillapex, Apatite Root Canal Sealer and AH26 sealers.Material and MethodsThe present in vitro study was carried out on 142 extracted single-rooted human mature teeth. The teeth were randomly divided into three experimental groups (n=44) and two control groups (n=5). Three root canal sealers were MTA Fillapex, Apatite Root Canal Sealer and AH26. The teeth in the control groups were either filled with no sealer or made completely impermeable. The root canals were prepared and obturated with gutta-percha and one of the sealers. The teeth were sterilized with ethylene oxide gas prior to the bacterial leakage assessment using Enterococcus faecalis. Leakage was evaluated every 24 hours for 90 days. Data were analyzed with descriptive statistical methods and chi-squared test. If the data were significant, a proper post hoc test was used. Statistical significance was set at P<0.05.ResultsThe positive control specimens exhibited total bacterial penetration whilst the negative control specimens showed no evidence of bacterial penetration. At the end of the study, the analysis of microleakage with chi-squared test showed no significant differences between the experimental groups (P<0.05). The results of chi-squared test analyzing the pair-wise differences between the groups considering the numerical values for leakage day indicated the lowest leakage with AH26 and the highest with Apatite root sealer.ConclusionsAccording to the results of the present study, sealing ability of AH26 was significantly higher than that of MTA Fillapex and Apatite Root Canal Sealer. Key words:Mineral Trioxide aggregate, root canal obturation, dental seal.

Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

“…Jafari et al, examined the sealing ability of MTA Fillapex, Apatite and AH 26 sealers by bacterial microleakage technique [20]. In this in vitro study, the lowest microleakage was related to AH 26 , MTA Fillapex and Apatite, respectively.…”

“…Some of these methods, such as dye penetration, are simple and some other, such as bacterial penetration, are more complex. However, in general, a standard technique has not been introduced yet, and even in the use of a particular method, differences in detail may be seen, which lead to contradictory results [20]. Among the available methods, the most commonly used method is dye penetration, which does not require sophisticated and advanced facilities [21,22].…”

Improved sealing ability promoted by calcium silicate-based root canal sealers

“…Endosequence BC Sealer presented a superior sealing ability than AH Plus in a dye penetration evaluation 14 , consisting with the present results. In contrast, MTA Fillapex has demonstrated controversial sealing performance in endodontic literature 13,[15][16][17][18] , what may result from its physicochemical properties 19 and differences in experimental designs such as the evaluation technique.…”

Successful Use of MTA Fillapex as a Sealant for Feline Root Canal Therapy of 50 Canines in 37 Cats

“…Most endodontic failures are due to bacterial or irritant microleakage from the root canal into the surrounding periapical tissues due to an inadequate apical seal. [25][26][27][28][29] The combination of a gutta percha point and sealant cement should ideally create a tridimensional, hermetic, fluid-tight, bacterial-impervious seal, 12,14,18,20,[30][31][32] preventing recontamination of the canal and the apical migration of bacteria and irritants. 1,[14][15][16] An ideal root canal sealant should have certain characteristics.…”

“…15,30 Among the currently available endodontic sealants, MTA Fillapex, a mineral trioxide aggregate (MTA) based root canal sealant cement, developed by Angelus, 36 possesses many of these characteristics. 30 An MTA Fillapex contains salicylate resin, diluent resin, natural resin, bismuth oxide, nanoparticulated silica, titanium dioxide, and MTA, 19,20,26,36 which is the main constituent and the important material in this composition.…”