In vitro evaluation of the effects of human umbilical cord extracts on human fibroblasts, keratinocytes, and melanocytes

Abstract

Skin aging is the result of internal and external factors. So-called photoaging has been identified as the major factor in skin aging. Effects of photoaging include inhibition of fibroblast and keratinocyte proliferation as well as collagen and fibronectin expression, while activating expression of collagenases such as matrix metalloproteinase-1. Previous studies have shown that extracts or products from human placenta significantly improve skin aging and chronic wound healing. However, there are few studies of umbilical cord extracts. Therefore, this study aimed to evaluate the effects of umbilical cord extract-derived formulae on three kinds of skin cells including fibroblasts, keratinocytes, and melanocytes. We prepared 20 formulae from intracellular umbilical cord extracts, extracellular umbilical cord extracts, and umbilical cord-derived stem cell extracts, as well as five control formulae. We evaluated the effects of the 25 formulae on fibroblast and keratinocyte proliferation, and expression of collagen I, fibronectin, and matrix metalloproteinase-1 in fibroblasts and tyrosinase in melanocytes. The results showed that 7.5% formula 35 was the most effective formula for promotion of fibroblast and keratinocyte proliferation. At this concentration, formula 35 also induced collagen expression and inhibited matrix metalloproteinase-1 expression at the transcriptional level. However, this formula had no effect on tyrosinase expression in melanocytes. These results demonstrate that umbilical cord extracts can serve as an attractive source of proteins for skincare and chronic wound healing products.

Elastin structure and its involvement in skin photoageing

“…Moreover, it is a visible organ that allows assessment of the health and well-being of an individual and reflects many aesthetic parameters [1][2][3]. Similar to other biological components of human body, the skin is subjected to a complex and cumulative ageing process throughout life, which occurs due to structural and physiological changes caused by factors that are intrinsic and extrinsic to the body [4][5][6][7][8][9].…”

Use of Human Umbilical Cord and Its Byproducts in Tissue Regeneration

“…Human umbilical cord can be mixed, filtrated or pelleted, the extract increases the in vitro proliferation and migration of fibroblasts, as described in the literature (Van Pham et al, 2014;Bakhtyar et al, 2017;Nagaishi et al, 2017;Saito et al, 2018). Original data from our laboratory have shown that increasing protein concentrations of HUC extract enhance fibroblast proliferation (after 48 h) and migration (16 h) (Figure 2).…”

Influence of cord blood serum and actovegin on the reproductive function of cows in the comparative aspect

“…In fact, CB is an integral part of fetal blood [5,6]. Doctors call umbilical cord blood liquid gold, scientists -the greatest success story in the field of cell technology [4,7].…”

“…CB also differs from adult blood in rheological and immunological characteristics [6,9], coagulation parameters [7], and oxygen transfer, which allows the use of fetal erythrocytes in severe forms of hemorrhagic shock accompanied by secondary tissue hypoxia [3].…”

“…CB erythrocytes have increased transport and immune properties and contain a large amount of fetal hemoglobin enriched with oxygen. Because of this, erythrocytes of the CB are able to function in the bloodstream for a long time after transfusion [7,10].…”