In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

Haryanto, Aris; Wihadmadyatami, Hevi; Wijayanti, Nastiti

doi:10.22146/ijbiotech.54703

Cited by 2 publications

(2 citation statements)

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“…In this work, the recombinant F protein of NDV had successfully in-vivo expressed into E. coli BL21(DE3) as a protein fragment with a molecular weight of 25.6 kDa under an optimal condition by 1.0 mM IPTG induction when the OD600 of the cell was 0.6 and an induction duration of 8 h. The results of this study were in line with Putri and Haryanto (2019), who carried out the in-vitro expression of NDV recombinant F protein from E. coli C-1a clone and Haryanto et al (2020), who successfully conducted in-vitro expression of the recombinant F protein of NDV from local Indonesian isolates by using a cell-free protein expression system. This finding also was concurrent with another researcher group Astuti et al (2020), who have successfully expressed and purify the recombinant F protein of local isolate NDV and studied the antibody response to recombinant F protein in broiler chicken post-vaccination.…”

Section: Production and Purification Of Recombinant F Protein In E Coli Bl21(de3)supporting

confidence: 76%

Recombinant fusion protein expression of Indonesian isolate Newcastle disease virus in Escherichia coli BL21(DE3)

et al. 2021

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Abstract. Wulanjati MP, Witasari LD, Wijayanti N, Haryanto A. 2021. Recombinant fusion protein expression of Indonesian isolate Newcastle disease virus in Escherichia coli BL21(DE3). Biodiversitas 22: 3249-3255. Newcastle disease is a major problem in poultry industry due to high mortality of susceptible chicken. New vaccine agents are important to be developed to eliminate the disease threat. This study aimed to examine the expression of fusion (F) protein of Newcastle Disease Virus (NDV) from Indonesian isolates in E. coli BL21(DE3) by IPTG induction. The sample was a part of F gene of NDV from Galur, Kulon Progo, Yogyakarta, Indonesia (0663/04/2013) with a molecular size of 600 bp that was synthesized and inserted into pBT7-N-His expression vector. The recombinant F protein with molecular weight of 25.6 kDa was successfully expressed in E. coli BL21(DE3), purified using Ni-NTA magnetic silica beads, and confirmed by western blotting. Optimization of expression showed that recombinant F protein was optimally expressed by induction of 1.0 mM IPTG when the cells reached OD600 = 0.6. The induction duration was 8 h. B-cell epitopes prediction showed that F protein possessed four epitopes that possibly recognized by B-cell. Since recombinant F protein was considered to possess immunogenicity, its potency as a candidate of NDV vaccine agent should be investigated in the future.

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Section: Production and Purification Of Recombinant F Protein In E Coli Bl21(de3)supporting

confidence: 76%

Recombinant fusion protein expression of Indonesian isolate Newcastle disease virus in Escherichia coli BL21(DE3)

et al. 2021

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“…It is regarded as an important reportable poultry disease and cause of economic loss (through morbidity, mortality, and production decline) in the poultry industry [9][10][11]. It hinders the global commercial poultry industry by causing significant illness and death in poultry [12,13].…”

Section: Introductionmentioning

confidence: 99%

Study on Seroprevalence and Associated Risk Factors of Newcastle Disease in Smallholder Poultry Farms in Sodo Zuria District, Wolaita Zone, Southern Ethiopia

Aliye

Endale

Mathewos

et al. 2022

Advances in Virology

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Newcastle disease (NCD) is a highly contagious viral disease of poultry and remains a constant threat in poultry farms that causes huge economic losses. The objective of this study was to estimate and assess the seroprevalence and associated risk factors of Newcastle disease in the Sodo Zuria district, southern Ethiopia. A cross-sectional survey followed by a simple random sampling technique was conducted from May to July 2021 on 384 apparently healthy nonvaccinated chickens on 30 smallholder poultry farms using commercial indirect ELISA kits and a questionnaire survey. The data were analyzed by using STATA for windows version 20 and a logistic regression reporting odds ratiowas applied to describe the seroprevalence of Newcastle disease with associated risk factors. The result of the study demonstrates that there was a high seroprevalence 48.7% (n= 187/384) of Newcastle disease in the study district. Information on associated risk factors were assessed using a semistructured questionnaire. The sex of the chicken showed a statistically significant difference (x2 = 4.842; p = 0.028) with the seroprevalence of the disease. The difference in seroprevalence among intensive, semi-intensive, and extensive management system was statistically significant (x2 = 3.84; p = 0.0001). There was also a statistical significant difference (x2 = 2.3854; p = 0.496) in the absence and presence of safe disposal of a dead chicken with the occurence of Newcastle disease. However, no statistically significant difference was observed among age groups (x2 = 4.335; p = 0.114), disinfection of poultry house (x2 = 0.0; p = 0.998), presence and absence of footbath (x2 = 2.969; p = 0.085), the breeds (x2 = 4.490; p = 0.106), type of chicken (x2 = 0.302; p = 0.583), and housing system (x2 = 1.926; p = 0.588). A high seroprevalence without vaccination history showed that the virus was circulating within the poultry. Therefore, further molecular study has to be conducted to identify circulating strains and develop an evidence-based control program.

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In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

Cited by 2 publications

References 29 publications

Recombinant fusion protein expression of Indonesian isolate Newcastle disease virus in Escherichia coli BL21(DE3)

Recombinant fusion protein expression of Indonesian isolate Newcastle disease virus in Escherichia coli BL21(DE3)

Study on Seroprevalence and Associated Risk Factors of Newcastle Disease in Smallholder Poultry Farms in Sodo Zuria District, Wolaita Zone, Southern Ethiopia

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