In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery

Yang, Chao; Sodian, Ralf; Fu, Ping; Lüders, Christine; Lemke, Thees; Du, Jing; Hübler, Michael; Weng, Y; Meyer, R.; Hetzer, Roland

doi:10.1016/j.athoracsur.2005.07.013

Cited by 34 publications

(32 citation statements)

References 16 publications

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“…Native porcine aortic valves cultured in an ex vivo pulsatile organ culture system maintained the native extracellular matrix composition of the leaflets while preserving the morphology and cell phenotype. In human, a pulsatile flow system in vitro using biodegradable patch scaffold was developed for cardiovascular surgery that provides biochemical and biomechanical signals in order to regulate autologous, human-tissue development in vitro (18). The application of patches or grafts in cardiovascular surgery, and particularly in pediatric cardiac surgery, is a widely accepted surgical technique for repair or reconstruction of cardiovascular structures (19,20).…”

Section: Discussionmentioning

confidence: 99%

The Porcine Aortic Tissue Culture System in vitro for Stem Cell Research

Kim

Yang

et al. 2011

Int J Stem Cells

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Background and Objectives: Due to the shortage of human donors for transplantation, the use of animal organs for xenotransplantation has come into great interest. Xeno-derived vessels and cardiac valves would be possible alternatives for the patient suffering from cardiovascular diseases. Therefore, we established in vitro culture system of a porcine vessel that could be helpful for the research of xenograft and stem cell research. Methods and Results: We primarily isolated porcine thoracic aorta, cultured square-shaped pieces up to 17 days and analyzed its morphology and characters. The endothelial cells were primarily isolated from cultured porcine aortic pieces and their morphology, function and character were analyzed in order to confirm them as endothelial cells at day 3, 4, 8, 10 and 17. Even at day 17, the morphology exhibited the intact endothelial layer as well as specifically expressed CD31 and von Willebrand factor. The morphology of primarily isolated cells from cultured tissues was identical as an endothelial cell. By flow cytometry analysis, more than 80% of the isolated cells expressed CD31 and up to 80% took up acetyl low density lipoprotein (ac-LDL) until day 10 of tissue culture period even though it decreased to about 50% at day 17 that means they not only showed typical endothelial cell characters but also functioned properly. Conclusions: We successfully established and optimized a porcine vascular tissue in vitro culture system that could be a valuable model for in vitro study of xenotransplantation and stem cell research.

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Section: Discussionmentioning

confidence: 99%

The Porcine Aortic Tissue Culture System in vitro for Stem Cell Research

Kim

Yang

et al. 2011

Int J Stem Cells

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“…Many groups are utilizing bioreactors to stimulate matrix production in engineered tissues before implantation. [61][62][63][64][65][66][67][68][69][70] It would be useful to be able to characterize tissues that are formed in a bioreactor and directly implanted rather than sacrificing a representative construct for histologic analysis. A further addition to such an approach could be the utilization of fluorescent reporter elements associated with the maturation of certain cell types.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Optical Signals Reveal Changes of Elastin and Collagen Organization During Differentiation of Mouse Embryonic Stem Cells

Thimm

Squirrell

Liu

et al. 2015

Tissue Engineering Part C: Methods

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“…The first clinical application of a tissue-engineered vascular patch with pretreatment of human bone marrow cells, was reported by Shin'oka (26). Likewise, tissue-engineered biodegradable materials with autologous cell seeding before patch repair, where a bioreactor culture system was used, have been well documented as potential cardiovascular patches (27)(28)(29)(30)(31)(32)(33). Oh and Lee reviewed hydrophilization of synthetic biodegradable polymeric scaffolds for improving cell/tissue compatibility (34).…”

Section: Cell-seeded Scaffoldsmentioning

confidence: 99%

Bioengineered Vascular Scaffolds: The State of the Art

et al. 2014

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To date, there is increasing clinical need for vascular substitutes due to accidents, malformations, and ischemic diseases. Over the years, many approaches have been developed to solve this problem, starting from autologous native vessels to artificial vascular grafts; unfortunately, none of these have provided the perfect vascular substitute. All have been burdened by various complications, including infection, thrombogenicity, calcification, foreign body reaction, lack of growth potential, late stenosis and occlusion from intimal hyperplasia, and pseudoaneurysm formation. In the last few years, vascular tissue engineering has emerged as one of the most promising approaches for producing mechanically competent vascular substitutes. Nanotechnologies have contributed their part, allowing extraordinarily biostable and biocompatible materials to be developed. Specifically, the use of electrospinning to manufacture conduits able to guarantee a stable flow of biological fluids and guide the formation of a new vessel has revolutionized the concept of the vascular substitute. The electrospinning technique allows extracellular matrix (ECM) to be mimicked with high fidelity, reproducing its porosity and complexity, and providing an environment suitable for cell growth. In the future, a better knowledge of ECM and the manufacture of new materials will allow us to “create” functional biological vessels - the base required to develop organ substitutes and eventually solve the problem of organ failure.

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In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery

Cited by 34 publications

References 16 publications

The Porcine Aortic Tissue Culture System in vitro for Stem Cell Research

The Porcine Aortic Tissue Culture System in vitro for Stem Cell Research

Endogenous Optical Signals Reveal Changes of Elastin and Collagen Organization During Differentiation of Mouse Embryonic Stem Cells

Bioengineered Vascular Scaffolds: The State of the Art

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