In Vitro Flowering and Fruiting of Capsicum fruitescens L.

Abstract

<jats:p>Flowering and fruit production were obtained from cultured shoot tips of `California Wonder', `Super Cayenne', and `Zippy' peppers grown in a liquid Murashige and Skoog (MS) medium without growth regulators. Thirty-day-old pepper shoot tips, derived from seedlings germinated sterilely, were cultured in a 6-liter polycarbonate container coupled to an automated plant culture system (APCS). Plants were given ten daily, 30-minute, compressed-air applications at 300 ml/minute. These shoots began flowering after an additional 60 to 90 days in culture and flowered continuously thereafter. About 5% to 10% of the flowers set fruit. Maximum fruit size obtained was ≈25% to 75% of the size of fruit produced on plants grown in vivo. In contrast, no flowering occurred from shoot tips grown in 25 × 150-mm culture tubes containing agar medium and subcultured every 8 weeks to fresh medium. Immature fruit excised from plantlets grown in the APCS were cultured separately on agar medium containing 0.0, 0.1, 0.3, or 1.0 mg BA/liter with and without 0.1 mg NAA/liter. Isolated fruit grew best on MS medium with BA only and poorest on medium without growth regulators. Chemical names used: <jats:italic>N</jats:italic>-(phenylmethyl)-1<jats:italic>H</jats:italic>-purine-6-amine (BA); 1-naphthaleneacetic acid (NAA).</jats:p>

In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (sweet basil) by axillary shoot proliferation

“…The floral morphology of the in vitro plants was normal and identical to the donor plants. In vitro flowering has been reported in a number of plant species such as Passiflora suberosa (Scorza and Janick, 1980), and Capsicumfruitescens (Tisserat and Galletta, 1995).…”

Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum

“…The in vitro flower induction and production of viable seeds from regenerated spinach shoots was reported (Al-Khayri et al 1991). Fruit production under in vitro conditions was obtained from cultured shoot tips of Capsicum fruitescens L. (Tisserat and Galletta 1995).…”

New approaches towards the shortening of generation cycles for faster breeding of protein legumeshh

“…In plant breeding, it is of value to accelerate generations by shortening each cycle (Roumet and Morin 1997), and also to induce flowering and seed set in vitro, particularly for rare and valuable genotypes where initial seeds are in very short supply (Al-Wareh et al 1989, Dickens and Van-Staden 1988, Tisserat and Galletta 1988. This would favour faster fixation of new genetic traits when regenerated shoots are difficult to root (Bean et al 1997) or when establishing regenerants is difficult, as seeds are harvested in vitro.…”