In vitro flowering and propagation ofLeptinella nanaL., an endangered plant

Abstract

Leptinella nana L., an endangered New Zealand plant, was successfully micropropagated in a half strength Murashige and Skoog (MS) medium containing 3% sucrose and no exogenous plant growth regulators. An in vitro multiplication rate of four to five plants per shoot subcultured every 3 weeks has been achieved. The plant cultures can be easily reintroduced to soil and grown under glasshouse conditions.Flowering occurred following subculture of the L. nana plants. This in vitro flowering capacity showed no sign of decline during 16 months of successive 3-weekly subcultures. Photoperiods, sucrose levels, and different MS salts do not appear to be the key controlling factors of the in vitro flowering process.

The Effects of Gamma Irradiation on the Growth and Propagation of <em>In-Vitro</em> Chrysanthemum Shoot Explants (cv. <em>Yellow Puma</em>)

“…The percentage of surviving explants both in the second and the third subcultures decreased significantly along with the increase of irradiation dose (see Table 3). The multiplication rate of control and explants exposed by 10 Gy was the same as results reported by Carson and Leung [14] in experiment on Leptinella nana L. in-vitro culture, i.e. four to five explants per shoot multiplication rate, which was sub-cultured every 3 weeks on MS medium.…”

“…The number of shoots in untreated explants higher than that of multiplication rate on the first subculture of Leptinella nana L. (Compositae family), which is five to six shoots every 3 weeks [14]. The difference is unusual because different species has different capability to produce shoots.…”

“…It was shown by stressed growth during the first cultures, both in number of leaves and number of branches produced. According to Carson and Leung [14], in average, number of leaves on the first shoot explants increased to 20 leaves after 17 days. The decrease of number of leaves and branches on chrysanthemum irradiated stem cuttings were also reported by Datta and Gupta [15], and Banerji and Datta [16].…”

In vitro flowering in Oldenlandia umbellata L.

“…In vitro flowering depends on various abiotic and genetic factors [27] . Insights on flower physiology and development can be easily accessed by in vitro flowering study [30] , [31] and premature seed setting and self incompatibility can also be studied. An ideal system to develop in vitro flowering if established can lead to rapid breeding of new varieties [32] , [33] and it has been reported in a number of plant species, for example in Withania somnifera [32] , Kinnow mandarin [33] , Streptocarpus nobilis [34] , Pharbitis nil [35] , Ammi majus [36] , Hypericum brasiliense [37] , Bambusa edulis [38] , [39] and Psygmorchis pusilla [40] ; however, there is no report to date on any species of Oldenlandia .…”