In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines

Doak, Shareen H.; Manshian, Bella B.; Jenkins, Gareth; Singh, Neenu

doi:10.1016/j.mrgentox.2011.09.013

Cited by 202 publications

(203 citation statements)

References 81 publications

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“…However, more in vivo studies are required to fully understand the mechanism of TiO 2 nanoparticles toxicity. Likewise, more caution should be considered for toxicity assessment of nanoparticles because of the limitations and potential artifacts of the results (Doak, et al, 2012, Landsiedel et al, 2009. This study provides a better insight into the potential toxicity of nanoparticles with respect to their physiochemical characteristics.…”

Section: Most Of the Toxicological Results On Nps Have Been Generatedmentioning

confidence: 92%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

124

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/npsi/ctrl?lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?lang=fr Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://dx.doi.org/10. 1016/j.tiv.2012.12.018 Toxicology in Vitro, 27, 2, pp. 864-873, 2012-12-28 In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells Hamzeh, Mahsa; Sunahara, Geoffrey I. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO 2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO 2 samples decrease cell viability in a mass-based concentration-and size-dependent manner, the polyacrylate-coated nano-TiO 2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO 2 model. Given the increasing production of TiO 2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.

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Section: Most Of the Toxicological Results On Nps Have Been Generatedmentioning

confidence: 92%

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Hamzeh

Sunahara

2013

Toxicology in Vitro

124

View full text Add to dashboard Cite

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“…Expressing the concentration in terms of mass/volume is the easiest option, but is however not always the most relevant 105 . Which was pointed out in a review by Johnston et Subsequently attention should go to standardisation of nanotoxicity methods in terms of incubation conditions like NP concentration and incubation time as overexposure conditions should be avoided 113 . The importance of avoiding overexposure conditions becomes clear when evaluating genotoxicity for example, as acute toxicity at overexposure levels can mistakenly be interpreted for genotoxicity since apoptosis itself induces DNA fragmentation 114,115 .…”

Section: Issues With Routine In Vitro Methodsmentioning

confidence: 99%

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

et al. 2013

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“…Relatively low toxicity has been found even when nanoparticle concentrations as high as 100 μg/mL were used, yet some studies have reported some level of damage to cells. 82,83 Basically, in vitro experiments have demonstrated the toxicity of SPIONs, causing cellular stress, [84][85][86][87][88] alterations in gene expression due to chromatin changes and genotoxicity, 82,89,90 decrease in cell proliferation, 83,85,87,88 and promotion of a proinflammatory environment. [91][92][93] In most studies, SPIONs do not exert any reported effects on adipogenic 94,95 and neurogenic differentiation.…”

mentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

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In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines

Cited by 202 publications

References 81 publications

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

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