IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

Dwiyani, Rindang; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, Ketut; Mayadewi, Ni Nyoman Ari

doi:10.17503/agrivita-2015-37-2-p144-150

Cited by 11 publications

(16 citation statements)

References 7 publications

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“…var. suavis from Bali obtained the highest number of plantlets by using NP (New Phalaenopsis) medium supplemented with 100 g L -1 tomato juice (Dwiyani, Yuswanti, Darmawati, Suada, & Mayadewi, 2015). It is promising that the in vitro cultivation (micropropagation) utilizing somatic embryogenesis process can produce plantlets that morphologically and genetically uniform.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4

et al. 2018

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To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation of the Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing orchid embryo). The three-week-old protocorms of D. phalaenopsis were genetically transformed with T-DNA carrying 35S :: GAL4 :: AtRKD4 :: GR through A. tumefaciens strain EHA 105. The cultures were maintained in VW medium with 10 mg L-1 Hygromycin. Due to the existence of glucocorticoid response element (GR) in the T-DNA construct, the transformed protocorms were transferred into VW medium with the addition of 15 µM Dexamethasone in 6 weeks after transformation to activate the transgene. A total of 12% protocorms has been confirmed for Hyg + by using PCR. The expression of embryo gene AtRKD4 was confirmed by cDNA analysis using AtRKD4 specific primers and Actin primers as a positive control experiment. The expression level of AtRKD4 in 2.5-month-old D. phalaenopsis transformant shoots was 7 times higher than nontransformant plants, and increased to 86 times higher in 8-months, that much higher than that of non-transformant. These results provide an improved method for genetic transformation of D. Phalaenopsis and will (eventually) increase production efficiency in the future.

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Section: Introductionmentioning

confidence: 99%

Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4

et al. 2018

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“…Coconut water contains various nutrients and phytohormones, especially cytokinin that make it an ideal additive supplement for plant tissue culture (Yong et al 2009). On the other hand, tomato extract is known to possess various vitamins and antioxidant lycopene (Semiarti et al 2010;Dwiyani et al 2015). Tomato extract is also known as a good buffer to stabilize pH value and has the ability to prevent iron precipitation in in vitro culture medium (Vacin and Went 1949).…”

Section: Discussionmentioning

confidence: 99%

“…The medium performance could be improved through addition of complex organic substance such as coconut water or tomato extract. Using said substances have successfully enhanced various orchid species growth (Zhang et al 2015;Dwiyani et al 2015;Setiari et al 2016;Utami et al 2017;Utami and Hariyanto 2019).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Germination and Flowering of Dendrobium capra J.J. Smith, An Endemic Orchid of Java

et al. 2021

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Dendrobium capra is an Indonesian endemic orchid species that live in Java. It grows on low altitude with warm climate. D. capra has beautiful small yellow greenish flower that grow in raceme inflorescence. This orchid faces a threat in its natural habitat due to having a long life cycle and a forestry main commodity as a main host thus categorized as Appendix II on CITES list. To address that problem, ex situ conservation approach using in vitro culture method is necessary. Germination enhancement effort using complex organic substances found that 200 ml/l tomato extract gave best germination result. Analysis on D. capra plantlet growth also showed that MS medium produced better plantlet size than NP, VW and KC medium. Supplementing medium with a combination of NAA and TDZ has also successfully induced early flowering within 11 month of culture period. This information is important to achieve successful in vitro culture of D. capra for various purposes.

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“…Genetic engineering is widely done by scientist lately. Despite its low success rate (Pardal, 2002, Utomo, 2004, genetic engineering is relatively fast (Dwiyani et al, 2016a), can be done with standard laboratory types of equipment (Rahmawati, 2006) and does not require spacious place compared to conventional cross-breeding (Azrai, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…and Agrobacterium tumefaciens-mediated transformation (Dwiyani et al, 2016a (Aldemita and Hodges, 1996).…”

Section: Introductionmentioning

confidence: 99%

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.)

Sanjaya

Dwiyani

Wirawan

et al. 2018

Int. J. Biosci. Biotech.

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One of the modern plant breedings through genetic engineering is Agrobacterium tumefaciens-mediated transformation. Agrobacterium tumefaciens-mediated transformation can be performed in vitro or in planta. In planta transformation arises from the weaknesses of the in vitro method such as need high hygiene standard, professional tissue culture experts, and more time to prepare explants and somaclonal variation. In planta transformation is a method to transfer the gene to the plant genome without any tissue culture stages. The aims of this research were to know the possibility of the prick and soak in planta method with the target of tomato seeds and to know the most suitable inoculation time for tomato seeds transformation by prick and soak method the transformation is done by pricking the seeds and soaking them in the A. tumefaciens suspension. The treatments in this study were 1 and 2 days inoculation time to test the efficacy of prick and soak in planta transformation method. Tomato seeds were pricked with a needle on the center once, and then soaked in A. tumefaciens strain LB4404 suspension carrying pKYS-SoSPS1 plasmid with Neomycin Phosphotransferase (NPTII) and Saccharum officinarum Sucrose Phosphate synthase (SoSPS1) genes. Visualization of tomato’s DNA samples after PCR showed that 1-day inoculation sample was positively integrated with NPTII gene and negative in the 2 days inoculation treatment.

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IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

Abstract: SVanda tricolor Lindl. var. suavis is an Indonesian wild orchid that has been rare in nature, so it needs attention to take care and conserve them.

Cited by 11 publications

References 7 publications

Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4

Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4

In Vitro Germination and Flowering of Dendrobium capra J.J. Smith, An Endemic Orchid of Java

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.)

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