SVanda tricolor Lindl. var. suavis is an Indonesian wild orchid that has been rare in nature, so it needs attention to take care and conserve them.

Darmawati IAP, Rai IN, Dwiyani R, Astarini IA. 2018. The diversity of wild Dendrobium (Orchidaceae) in Central Bali,Indonesia. Biodiversitas 19: 1110-1116. Dendrobium is the largest orchid genus of the Orchidaceae family. The research aimed toexplore the species diversity of Dendrobium, dominance, and species similarity in some forest of Bali. The explored locations weregrouped to lowland about 0-700 m asl. (Tejakula, Bukit Silangjana, Sepang Kelod, Mekori, and Busungbiu), transition land about 700-1100 m asl. (Sepang Kaje and Jatiluwih) and lower montane 1100-1500 m asl. (Natural Reserves of Lake Buyan-Tamblingan, PuncakLandep, and Lemukih). There were 24 species of Dendrobium in Central Bali, 2 spesies of which were not identified. The dominantspecies were D. acuminatissimum, D. crumenatum and D. linearifolium. The highest species diversity index of wild Dendrobium waspresent in lowland (16 species, 66.66%) followed by those in transition land and lower montane (12 species, 50.00% and 6 species,20.83%, respectively). The pattern is also similar either for the species prosperity (3.875; 3.094, and 0.699, respectively). Uniformity ofwild Dendrobium on transition land was 0.922, followed by those in lowland and lower montane (0.864 and 0.794). There were 6similar species of Dendrobium found in lowlands and lower montane (42.857% equality index) and 2 similar species in lowlands andlower montane (19.047% equality index) and also 2 similar species in transition land and lower montane (23.359% equality index). D.macrophylum, D. heterocarpum and D.secundum, their presence are pronely threatened with extinction so conservation is necessary.

Abstract. Darmawati IAP, Astarini IA, Yuswanti H, Fitriani Y. 2021. Conservation of Dendrobium spp. Bali orchids (Indonesia) through in vitro seed culture. Biodiversitas 22: 2554-2559. Orchid species, including Dendrobium spp., originated from Bali are threatened to extinction due to habitat destruction, illegal hunting, small population size, and the effects of global environmental change. Conservation strategies are therefore required to preserve the remaining germplasm, one of which is through in vitro seed propagation. This research aimed to investigate the pollination compatibility (i.e., pollination that produce fruits) of Dendrobium orchids originated from Bali, and to determine the effects of adding organic matters (i.e., coconut water, tomato extract, and peptone) to the base media on orchid seed germination under in vitro culture condition. Self-pollination and sibling pollination were implemented to seven orchid species, namely Dendrobium macrophyllum, D. heterocarpum, D. fimbriatum, D. linearifolium, D. spathilingue, D. secundum, and D. plicatile. The results showed that only four species, i.e., D. macrophyllum, D. heterocarpum, D. fimbriatum, and D. linearifolium, that produced fruits after being pollinated. The subsequent in vitro propagation of seeds produced by the four species showed that the addition of organic matters of tomato extract, coconut water, and peptone significantly affected the germination of Dendrobium forma Bali orchid seeds, indicated by different germination rates. The addition of 20% coconut water to Vacin and Went (VW) media gave the highest percentages of seed germination and protocorm formation for D. macrophyllum, D. heterocarpum, and D. fimbriatum at 98.33% and 95.00%; 91.67% and 91.67%; and 95% and 98.33%, respectively. Our findings can serve as baseline information when developing conservation strategies of Dendrobium orchids from Bali, particularly from the aspect of propagation

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.

The studies of the Bulung Boni and Bulung Anggur (Caulerpa spp.) species and secondary metabolites are still very limited. Proper identification will support various aspects, such as cultivation, utilization, and economic interests. Moreover, understanding the secondary metabolites will assist in developing algae-based products. This study aimed to identify these indigenous Caulerpa algae and analyze their bioactive components. The tufA sequence was employed as a molecular marker in DNA barcoding, and its bioactive components were identified using the GC-MS method. The phylogenetic tree was generated in MEGA 11 using the maximum likelihood method, and the robustness of the tree was evaluated using bootstrapping with 1000 replicates. This study revealed that Bulung Boni is strongly connected to Caulerpa cylindracea. However, Bulung Anggur shows no close relationship to other Caulerpa species. GC-MS analysis of ethanolic extracts of Bulung Boni and Bulung Anggur showed the presence of 11 and 13 compounds, respectively. The majority of the compounds found in these algae have been shown to possess biological properties, such as antioxidant, antibacterial, anticancer, anti-inflammation, and antidiabetic. Further study is necessary to compare the data obtained using different molecular markers in DNA barcoding, and to elucidate other undisclosed compounds in these Caulerpa algae.