In vitro hemocompatibility of albumin–heparin multilayer coatings on polyethersulfone prepared by the layer‐by‐layer technique

Abstract

Polyethersulfone foils (PES)--a unique material for blood purification membranes--were coated with a multilayer assembly of heparin (unfractionated or high anticoagulant activity fraction heparin) and albumin (albumin-heparin coatings), or with a multilayer of albumin (albumin coating), using the layer-by-layer technique. The coatings combine advantages of albumin (reduction of nonspecific interactions) and heparin (specific interactions with blood coagulation proteins). The differences between the two heparins, while significant for their biological activity, had only a minor effect on the multilayer assembly with albumin monitored in situ by reflection infrared spectroscopy (FTIR MIRS). Uncoated as well as modified PES surfaces were evaluated using an in vitro assay with freshly drawn, slightly heparinized (1.5 IU heparin/mL) human whole blood. The blood was circulated with a roller pump over the sample surfaces in shear flow across rectangular slit channels ( app. 6 mL/min and 120 s(-1)) for 1.5 h at 37 degrees C. All coatings effectively reduced platelet adhesion and activation according to the PF4 release. The activation of coagulation evaluated as TAT generation was significantly lowered for the coating composed of albumin and high activity heparin. A further beneficial effect of the heparin containing coatings was reduced complement activation as determined by different complement fragments.

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

“…[ 104,105 ] PEMS can rapidly and effi ciently coat surfaces of any shape and size and any charged species including natural proteins and peptides, synthetic polymers, polypeptides, DNA and growth factors, can be used. [104][105][106] PEMs are [168,169] is the use of the hydroquinone/quinone redox pair. In the oxidized form, the quinone can react further with dienes (e.g., cyclopentadiene) in a Diels-Alder reaction to couple other moieties such as RGD to the quinine.…”

Surface immobilization of chondroitin 6-sulfate/heparin multilayer on stainless steel for developing drug-eluting coronary stents

“…Immobilized HEP can also activate AT III, and hence in turn prevent prothrombin to become thrombin. Both mechanisms can hinder the coagulation cascade to improve hemocompatibility, as reported in the literature [13][14][15][16][17][18][19][20]. Furthermore, the releasing rate of loaded sirolimus can be controlled by the number of layers to inhibit SMCs growth.…”

“…Polymers grafted with heparin exhibits improved hemocompatibility [13][14][15][16][17][18][19]. To produce a more durable anticoagulant surface, modification of materials by the incorporation of sulfonate groups, so called heparin-like materials (heparinoid), has also been attempted by many investigators [13][14][15][16][17][18][19].…”

Surface modification of polysulfone based hemodialysis membranes with layer by layer self assembly of polyethyleneimine/alginate-heparin: a simple polyelectrolyte blend approach for heparin immobilization

“…The proposed strategy can be an economical alternative for the modification of hemodialysis membranes since HEP is an expensive compound compared to ALG. In previous studies, the LBL deposition of HEP with different polyelectrolytes such as chitosan, collagen, dextrin sulfate and albumin on different blood-contacting surfaces has been carried out [18,[25][26][27][28][29]. In these studies, HEP was used alone not only in the last layer of the assembly but also in each alternating layer to provide charge reversal of the polycation layer.…”

“…The method was used to improve biocompatibility of blood-contacting materials. For example, platelet adhesion/activation and leukocyte adhesion on polyethersulfone hemodialysis membrane were significantly reduced with the LBL deposition of heparin and albumin on the surface of this membrane [18].…”